LOCUS BC032740 3274 bp mRNA linear HUM 24-MAR-2004
DEFINITION Homo sapiens RAB5B, member RAS oncogene family, mRNA (cDNA clone
MGC:45320 IMAGE:5538490), complete cds.
ACCESSION BC032740
VERSION BC032740.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3274)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3274)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 69 Row: l Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4506370.
FEATURES Location/Qualifiers
source 1..3274
/db_xref="H-InvDB:HIT000259047"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:45320 IMAGE:5538490"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3274
/gene="RAB5B"
/db_xref="GeneID:5869"
/db_xref="MIM:179514"
CDS 129..776
/gene="RAB5B"
/codon_start=1
/product="RAB5B protein"
/protein_id="AAH32740.1"
/db_xref="GeneID:5869"
/db_xref="MIM:179514"
/translation="MTSRSTARPNGQPQASKICQFKLVLLGESAVGKSSLVLRFVKGQ
FHEYQESTIGAAFLTQSVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDI
TNQETFARAKTWVKELQRQASPSIVIALAGNKADLANKRMVEYEEAQAYADDNSLLFM
ETSAKTAMNVNDLFLAIAKKLPKSEPQNLGGAAGRSRGVDLHEQSQQNKSQCCSN"
misc_feature 192..677
/gene="RAB5B"
/note="Ras; Region: Ras family. Includes sub-families Ras,
Rab, Rac, Ral, Ran, Rap Ypt1 and more. Shares P-loop motif
with GTP_EFTU, arf and myosin_head. See pfam00009
pfam00025, pfam00063. The high cutoff is so high to avoid
overlaps with related families"
/db_xref="CDD:pfam00071"
BASE COUNT 778 a 853 c 726 g 917 t
ORIGIN
1 ccacgcgtcc ggtgcacata tagcccaccc tcgaaggagt gttgaagcct ggaaatcccc
61 tccccttccc cctcccccct ttacagtatc cccctccctc caccctttcc cattctgata
121 atctggccat gactagcaga agcacagcta ggcccaatgg gcaaccccag gccagcaaaa
181 tttgccagtt caaattggtc ctgctgggag aatctgcagt gggaaagtca agcctggtat
241 tacgttttgt caaagggcag ttccatgagt accaggagag caccattgga gcggccttcc
301 tcacccagtc cgtttgtcta gatgacacaa cagtgaagtt tgagatctgg gacacagctg
361 ggcaggagcg atatcacagc ttagccccca tgtactacag gggtgcccaa gctgcaatcg
421 tggtttacga cattactaat caggaaacct ttgcccgagc aaagacatgg gtgaaggaac
481 tacagcgaca ggccagtcct agcatcgtta ttgccctggc agggaacaaa gctgacctgg
541 ccaacaaacg tatggtggag tatgaagagg cccaggcata tgcagatgac aacagcttat
601 tgttcatgga gacttcagcc aagacagcta tgaacgtgaa tgatctcttc ctggcaatag
661 ctaagaagtt gccaaagagt gaaccccaga atctgggagg tgcagcaggc cgaagccggg
721 gtgtggatct ccatgaacag tcccagcaga acaagagcca gtgttgtagc aactgagggg
781 gtggctagca gcaaacaagt atggagctag cacaagagct aagaaataac ctccatccct
841 acccctcagc acacaacccc tacggtaaca gcacactgag ccctggctcc caagggctgc
901 ctcctgacag ctccgtcatg gcacttttta acgcttcagc aacaaacacc aggcagctgt
961 tgccactggc ctcctacccc ctactctggg gcttgggggt caactccccc caggacttac
1021 cttccaaaac aaactttctt cactttgtat tataggtaca agacagcgac ttacgtatct
1081 tttctcctcc tccctagtgt tcctccccat tttttcagaa aacacttctg actcctgtcc
1141 cttccccttc tgcttttggt cagtccctgt tcttgagcct cttttctcct ctccccagga
1201 tgcagaaagt ggtgaaccca ggaactgagg aaggaggttt ccagttcatt tacattaagg
1261 gccctggggg agaataaagc tcagagcagg agggagtaag gaaacatttc ctttttgttt
1321 ttatttggtt ggagtttctc atatttgaaa acattgcggt atccatgatt tggccttgtg
1381 gagggtgttc ctaggtagag gtgagaatgg ggaggcaaga tctcaggcac caggcaggag
1441 gtgccttgta agctaactgg gcggaggtgg aggtgcagtg tcaactgtgg ctctgtaact
1501 cttcaaaggc ccagtttccc ctcacgcagc ctcttaggta gcgtttcccc taatcgtggg
1561 ggttggaccc cagagtcttc caaagaattt tcactggttg cctgcatctt tggctctgct
1621 gtgatctgat tggaggaggg acagtttctg gtacccatcc tctgatttat acatatgcat
1681 tttttcccct ctggccttta gatggcctca gccccagcca ccatataccc ctgcagtttg
1741 cactttaatt gatggtagtt cagttggggt acttgtttta tggaagtttt gattgattta
1801 cttgccctcc caccttcttt ttaattcaat gaaatctgag gttaatgcga ggttcgagga
1861 gaggttatag ataaaactac cagtggcagc tactcaagtc ctatctccac tgttagcttc
1921 ctccaactct aattattaac ctatattctt gccaagctaa ctattgacta taggtttgcc
1981 tttcctggag aattaattga gcaattgagg agtgtctcag gatagcacag gccaaggtag
2041 gggagtaaaa aggaggtcag gcaaaaggga ggagttttct gtcctttccc aggtttcaca
2101 ctcaatttga tatccattac catgtctttt ctacttcctt gtaaataggt atgatcttta
2161 ttcccactgt acagtctgtt ctatcctctg cctcccatca ggccctgttt ctttgttcct
2221 ttgttaatat cttgaattta gtccctccat ccttaatccc cccatccctc cccatcatgc
2281 aaccagtggt ttaatccatg taccaatagg ggctagtacc acagaggcct cctgtggtgc
2341 cctcgtatca taccacctgt tcctgtggag agggaatgac cggcactgaa ggtaccttac
2401 aactggctca tattatcaga ggaccttggt cctttctaaa tctctagtct ctcttcatat
2461 ccttcatcag gtgttttaag atgtctctga gaagccatca aggcaaaaga gaactttaag
2521 ttccttgttc cagcccggag ttttgggaaa gaaagaaagg aaaggtcaca gtgacctagg
2581 attggaacct tcctgccctt ttggcttgca gactgccttc tatcccagaa cagctgagaa
2641 atctatgaag ctgagattct gaaggaccca gcttaggttc ttccacttag gcctcaattc
2701 ccttcctttt ccaggggcag ccttagttcc catggccctg aaacacacac atttccccct
2761 tcctttccca gaagccactg gccccccata gcacccagtg catccttttt acaagtggaa
2821 gaactaggat ggctttccaa agtcttctag aaatgaagtt ctttctctgt gcagctttcc
2881 cccttggagc aggagtgaag atgtttcatt atcttgggcc tgggaaacca cttccccagg
2941 cttctccctc cccccacccc cataggaaca ggatttggcc ttagcttctg ggcctatcgg
3001 ctgccttccc tctacttcct accacctctt ctgccttcct ttgagctctg ttgggcttgg
3061 ggatcttagt tttcttttgt ttatttccca gctcattttt ttcttctggt cagttttttt
3121 aagggggggt gttgtggttt tttgtttttg ttttgcttct gagaaagcat ttgcctttct
3181 tcctctccca acataacaat cgtggtaaca gaatgcgact gctgatttac cgatgtattt
3241 aatgtaagta aaaaaaggaa aaaaaaaaaa aaaa
//