LOCUS BC032639 4149 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens eukaryotic translation initiation factor 5B, mRNA
(cDNA clone MGC:44860 IMAGE:5496504), complete cds.
ACCESSION BC032639
VERSION BC032639.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4149)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4149)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Lou Staudt
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 69 Row: f Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 15451891.
FEATURES Location/Qualifiers
source 1..4149
/db_xref="H-InvDB:HIT000041647"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:44860 IMAGE:5496504"
/tissue_type="Lymph, lymphoma"
/clone_lib="NIH_MGC_85"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4149
/gene="EIF5B"
/gene_synonym="DKFZp434I036"
/gene_synonym="FLJ10524"
/gene_synonym="IF2"
/gene_synonym="KIAA0741"
/db_xref="GeneID:9669"
/db_xref="HGNC:HGNC:30793"
/db_xref="MIM:606086"
CDS 107..3769
/gene="EIF5B"
/gene_synonym="DKFZp434I036"
/gene_synonym="FLJ10524"
/gene_synonym="IF2"
/gene_synonym="KIAA0741"
/codon_start=1
/product="eukaryotic translation initiation factor 5B"
/protein_id="AAH32639.1"
/db_xref="GeneID:9669"
/db_xref="HGNC:HGNC:30793"
/db_xref="MIM:606086"
/translation="MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKK
KKEKKKQDFDEDDILKELEELSLEAQGIKADRETVAVKPTENNEEEFTSKDKKKKGQK
GKKQSFDDNDSEELEDKDSKSKKTAKPKVEMYSGSDDDDDFNKLPKKAKGKAQKSNKK
WDGSEEDEDNSKKIKERSRINSSGESGDESDEFLQSRKGQKKNQKNKPGPNIESGNED
DDASFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQKESQRKF
EEETVKSKVTVDTGVIPASEEKAETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKK
GPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQ
KEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKK
RKKIPQQLESKEVSESMELCAAVEVMEQGVPEKEETPPPVEPEEEEDTEDAGLDDWEA
MASDEETEKVEGNTVHIEVKENPEEEEEEEEEEEEDEESEEEEEEEGESEGSEGDEED
EKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNV
NTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQT
KMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIES
INLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEF
AQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEEL
RAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELR
VKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLN
AIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQ
YAVNLAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIA
VFPCKIKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHK
QVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQK
SDWQLIVELKKVFEII"
BASE COUNT 1611 a 638 c 1009 g 891 t
ORIGIN
1 ccacgcgtcc gaaaagagct gagcggagac caaagtcagc cgggagacag tgggtctgtg
61 agagaccgaa tagaggggct ggggccacga gcgccattga caagcaatgg ggaagaaaca
121 gaaaaacaag agcgaagaca gcaccaagga tgacattgat cttgatgcct tggctgcaga
181 aatagaagga gctggtgctg ccaaagaaca ggagcctcaa aagtcaaaag ggaaaaagaa
241 aaaagagaaa aaaaagcagg actttgatga agatgatatc ctgaaagaac tggaagaatt
301 gtctttggaa gctcaaggca tcaaagctga cagagaaact gttgcagtga agccaacaga
361 aaacaatgaa gaggaattca cctcaaaaga taaaaaaaag aaaggacaga agggcaaaaa
421 acagagtttt gatgataatg atagcgaaga attggaagat aaagattcaa aatcaaaaaa
481 gactgcaaaa ccgaaagtgg aaatgtactc tgggagtgat gatgatgatg attttaacaa
541 acttcctaaa aaagctaaag ggaaagctca aaaatcaaat aagaagtggg atgggtcaga
601 ggaggatgag gataacagta aaaaaattaa agagcgttca agaataaatt cttctggtga
661 aagtggtgat gaatcagatg aatttttgca atctagaaaa ggacagaaaa aaaatcagaa
721 aaacaagcca ggtcctaaca tagaaagtgg gaatgaagat gatgacgcct ccttcaaaat
781 taagacagtg gcccaaaaga aggcagaaaa gaaggagcgc gagagaaaaa agcgagatga
841 agaaaaagcg aaactgcgga agctgaaaga aaaagaagag ttagaaacag gtaaaaagga
901 tcagagtaaa caaaaggaat ctcaaaggaa atttgaagaa gaaactgtaa aatccaaagt
961 gactgttgat actggagtaa ttcctgcctc tgaagagaaa gcagagactc ccacagctgc
1021 agaagatgac aatgaaggag acaaaaagaa gaaagataag aagaaaaaga aaggagaaaa
1081 ggaagaaaaa gagaaagaga agaaaaaagg acctagcaaa gccactgtta aagctatgca
1141 agaagctctg gctaagctta aagaggaaga agaaagacag aagagagaag aggaagaacg
1201 tataaaacgg cttgaagaat tagaagccaa gcgtaaagaa gaggaacgat tggaacaaga
1261 aaaaagagaa aggaaaaagc aaaaagaaaa agaaagaaaa gaacgcttga aaaaagaagg
1321 gaaactttta actaaatccc agagagaagc cagagccaga gccgaagcta ctcttaaact
1381 gctacaagct cagggtgttg aagtgccatc aaaagactct ttgccaaaga agaggccaat
1441 ttatgaagat aaaaagagga aaaaaatacc acagcagcta gaaagtaaag aagtgtctga
1501 atcaatggaa ttatgtgctg ctgtagaagt tatggaacaa ggagtaccag aaaaggaaga
1561 gacaccacct cctgttgaac cagaagaaga agaagatact gaggatgctg gattggatga
1621 ttgggaagct atggccagtg atgaggagac agaaaaagta gaaggaaaca cagttcatat
1681 agaagtaaaa gaaaaccctg aagaggagga ggaggaggaa gaagaggaag aagaagatga
1741 agaaagtgaa gaagaggagg aagaggaggg agaaagtgaa ggcagtgaag gtgatgagga
1801 agatgaaaag gtgtcagatg agaaggattc agggaagaca ttagataaaa agccaagtaa
1861 agaaatgagc tcagattctg aatatgactc tgatgatgat cggactaaag aagaaagggc
1921 ttatgacaaa gcaaaacgga ggattgagaa acggcgactt gaacatagta aaaatgtaaa
1981 caccgaaaag ctaagagccc ctattatctg cgtacttggg catgtggaca cagggaagac
2041 aaaaattcta gataagctcc gtcacacaca tgtacaagac ggtgaagcag gtggtatcac
2101 acaacaaatt ggggccacca atgttcctct tgaagctatt aatgaacaga ctaagatgat
2161 taaaaatttt gatagagaga atgtacggat tccaggaatg ctaattattg atactcctgg
2221 gcatgaatct ttcagtaatc tgagaaatag aggaagctct ctttgtgaca ttgccatttt
2281 agttgttgat attatgcatg gtttggagcc ccagacaatt gagtctatca accttctcaa
2341 atctaaaaaa tgtcccttca ttgttgcact caataagatt gataggttat atgattggaa
2401 aaagagtcct gactctgatg tggctgctac tttaaagaag cagaaaaaga atacaaaaga
2461 tgaatttgag gagcgagcaa aggctattat tgtagaattt gcacagcagg gtttgaatgc
2521 tgctttgttt tatgagaata aagatccccg cacttttgtg tctttggtac ctacctctgc
2581 acatactggt gatggcatgg gaagtctgat ctaccttctt gtagagttaa ctcagaccat
2641 gttgagcaag agacttgcac actgtgaaga gctgagagca caggtgatgg aggttaaagc
2701 tctcccgggg atgggcacca ctatagatgt catcttgatc aatgggcgtt tgaaggaagg
2761 agatacaatc attgttcctg gagtagaagg gcccattgta actcagattc gaggcctcct
2821 gttacctcct cctatgaagg aattacgagt gaagaaccag tatgaaaagc ataaagaagt
2881 agaagcagct cagggggtaa agattcttgg aaaagacctg gagaaaacat tggctggttt
2941 acccctcctt gtggcttata aagaagatga aatccctgtt cttaaagatg aattgatcca
3001 tgagttaaag cagacactaa atgctatcaa attagaagaa aaaggagtct atgtccaggc
3061 atctacactg ggttctttgg aagctctact ggaatttctg aaaacatcag aagtgcccta
3121 tgcaggaatt aacattggcc cagtgcataa aaaagatgtt atgaaggctt cagtgatgtt
3181 ggaacatgac cctcagtatg cagtaaattt ggccttcgat gtgagaattg aacgagatgc
3241 acaagaaatg gctgatagtt taggagttag aatttttagt gcagaaatta tttatcattt
3301 atttgatgcc tttacaaaat atagacaaga ctacaagaaa cagaaacaag aagaatttaa
3361 gcacatagca gtatttccct gcaagataaa aatcctccct cagtacattt ttaattctcg
3421 agatccgata gtgatggggg tgacggtgga agcaggtcag gtgaaacagg ggacacccat
3481 gtgtgtccca agcaaaaatt ttgttgacat cggaatagta acaagtattg aaataaacca
3541 taaacaagtg gatgttgcaa aaaaaggaca agaagtttgt gtaaaaatag aacctatccc
3601 tggtgagtca cccaaaatgt ttggaagaca ttttgaagct acagatattc ttgttagtaa
3661 gatcagccgg cagtccattg atgcactcaa agactggttc agagatgaaa tgcagaagag
3721 tgactggcag cttattgtgg agctgaagaa agtatttgaa atcatctaat tttttcacat
3781 ggagcaggaa ctggagtaaa tgcaatactg tgttgtaata tcccaacaaa aatcagacaa
3841 aaaatggaac agacgtattt ggacactgat ggacttaagt atggaaggaa gaaaaatagg
3901 tgtataaaat gttttccatg agaaaccaag aaacttacac tggtttgaca gtggtcagtt
3961 acatgtcccc acagttccaa tgtgcctgtt cactcacctc tcccttcccc aacccttctc
4021 tacttggctg ctgttttaaa gtttgccctt ccccaaattt ggatttttat tacagatcta
4081 aagctctttc gattttatac tgattaaatc agtactgcag tatttgatta accaaaaaaa
4141 aaaaaaaaa
//