LOCUS BC032603 2516 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens chromosome 5 open reading frame 54, mRNA (cDNA clone
MGC:44989 IMAGE:5535434), complete cds.
ACCESSION BC032603
VERSION BC032603.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2516)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2516)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 69 Row: k Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, GenomeScan gene prediction.
FEATURES Location/Qualifiers
source 1..2516
/db_xref="H-InvDB:HIT000051060"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:44989 IMAGE:5535434"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2516
/gene="C5orf54"
/gene_synonym="Buster3"
/db_xref="GeneID:63920"
/db_xref="HGNC:HGNC:30804"
CDS 530..2314
/gene="C5orf54"
/gene_synonym="Buster3"
/codon_start=1
/product="C5orf54 protein"
/protein_id="AAH32603.1"
/db_xref="GeneID:63920"
/db_xref="HGNC:HGNC:30804"
/translation="MSKKRKWDDDYVRYWFTCTTEVDGTQRPQCVLCNSVFSNADLRP
SKLSDHFNRQHGGVAGHDLNSLKHMPAPSDQSETLKAFGVASHEDTLLQASYQFAYLC
AKEKNPHTVAEKLVKPCALEIAQIVLGPDAQKKLQQVPLSDDVIHSRIDEMSQDILQQ
VLEDIKASPLKVGIQLAETTDMDDCSQLMAFVRYIKEREIVEEFLFCEPLQLSMKGID
VFNLFRDFFLKHKIALDVCGSVCTDGASSMLGENSEFVAYVKKEIPHIVVTHCLLNPH
ALVIKTLPTKLRDALFTVVRVINFIKGRAPNHRLFQAFFEEIGIEYSVLLFHTEMRWL
SRGQILTHIFEMYEEINQFLHHKSSNLVDGFENKEFKIHLAYLADLFKHLNELSASMQ
RTGMNTVSAREKLSAFVRKFPFWQKRIEKRNFTNFPFLEEIIVSDNEGIFIAAEITLH
LQQLSNFFHGYFSIGDLNEASKWILDPFLFNIDFVDDSYLMKNDLAELRASGQILMEF
ETMKLEDFWCAQFTAFPNLAKTALEILMPFATTYLCELGFSSLLHFKTKSRSCFNLSD
DIRVAISKKVPRFSDIIEQKLQLQQKSL"
BASE COUNT 766 a 484 c 527 g 739 t
ORIGIN
1 ccacgcgtcc gccgggcctg gctgaggcgg aggttgttgc ggatgcttct ctctccaggc
61 gcaccgcaga ttcctgcccg ggggttggac ccgaggaacc gccgtctcta ctggttccga
121 cggccagagg ctgggcccta ggctgagcgg agagtgggac tggagcctga aacgggcgcc
181 taaaagaagc ccgtgcatcc cataggcctg cgcctcaggc ttggagacgg gcgggcggag
241 accgcgctga gtcttttttt ttttcctagc agctggcgtt cttatctcac ccgggaggct
301 gtaaaaatct ccgtccttaa cagtaacacc atcaggtcct atcagttaag agctgcttgt
361 tatttgcatg cattcttgta attaaatctc ctaacaaccc tgtaagccac attctctggc
421 acagaacttt ggaaactttg gggatcactt acttatcttg gagacagaat gttttctaag
481 caaaattctg tggcatctct ggaatactac ctgagaattt ttgtgtgcca tgtcgaagaa
541 acgcaaatgg gatgatgact atgttcgcta ctggttcacc tgtacaacgg aagtagatgg
601 aactcagcgt ccacagtgtg tgttgtgtaa ctcagtattt tcaaatgctg acctcagacc
661 atcaaaactg tcagaccatt ttaacagaca gcatggtggt gtagctgggc atgatctcaa
721 tagcctgaag catatgccag caccatctga tcagagtgaa accttgaaag catttggagt
781 tgcatctcat gaggatactt tattacaagc atcgtatcaa tttgcgtatt tatgtgccaa
841 ggagaagaat cctcatacag tagctgaaaa gttagtgaaa ccttgtgcac tggaaatagc
901 acaaatagtt ttgggaccag atgcacaaaa aaagcttcag caggtaccct tatcagatga
961 tgtgatccat tctagaattg atgaaatgag ccaggatatc ttacagcaag ttctagaaga
1021 tatcaaagcc agtcctctta aagtgggtat tcagcttgct gagacaactg acatggatga
1081 ctgcagtcag ctaatggcat ttgtgcgcta tataaaagaa agagagatcg tagaagaatt
1141 tctcttctgt gaaccattgc agctatccat gaaaggaata gatgtgttca atctcttcag
1201 agacttcttt ctgaagcata agatagcact tgatgtatgt ggctctgttt gtactgatgg
1261 tgcctcctct atgctaggag aaaattccga gtttgttgcc tacgtgaaaa aagagatacc
1321 tcatatcgta gttacacatt gtttattgaa tcctcatgca cttgtcataa agacattgcc
1381 tacaaaactg agggatgctc tgtttactgt ggtgagggta ataaatttca tcaaagggag
1441 agctccaaat catcgcctat ttcaggcttt ctttgaagaa attggcatag aatatagtgt
1501 cctccttttc catactgaaa tgaggtggct ttcccgaggc caaatactta ctcacatttt
1561 tgaaatgtat gaagaaataa atcagtttct tcaccacaag agcagtaatt tagttgatgg
1621 ctttgaaaat aaagagttta aaattcacct agcatacctt gcagatttat tcaaacactt
1681 aaatgaactc agtgcatcta tgcagaggac tgggatgaac acagtatcag ctagagagaa
1741 gttatctgct tttgttagga agtttccatt ttggcaaaaa cgaattgaga aaagaaattt
1801 taccaatttt ccttttcttg aagaaataat tgtttcagat aatgaaggca tattcattgc
1861 agctgaaata acactgcatc tgcaacaatt gagcaacttc ttccatggat atttttccat
1921 tggagatctt aatgaggcaa gtaaatggat attggatcca tttcttttta atatcgattt
1981 tgttgatgat agttatttaa tgaaaaatga tcttgctgaa ttacgagcta gtggccaaat
2041 cctaatggaa tttgagacaa tgaagcttga ggatttctgg tgtgcccaat tcacagcatt
2101 tccaaacctg gcaaagacag ctctagaaat ccttatgcca tttgcaacta catacctttg
2161 tgagttggga ttttcatcac ttttacattt caaaacaaag tccagaagct gctttaatct
2221 gagtgatgat atccgtgtgg ctatttcaaa aaaagttcct cgtttctcgg acatcattga
2281 acaaaagcta cagctacagc agaagtcact gtaagctgat atactttttt aatgaataat
2341 ttcaatctta atatcattgt aaaattaagc tgtaattctg tctctttcat atttatgata
2401 tactgaattc tatgtctaaa aaacttaaga actttaattt tgaatgaggc atgtgaaaat
2461 atgttttgag aataaaaaca cagcagaaaa gaaaaaaaaa aaaaaaaaaa aaaaaa
//