LOCUS BC032597 3368 bp mRNA linear HUM 18-JAN-2018
DEFINITION Homo sapiens neural precursor cell expressed, developmentally
down-regulated 4-like, mRNA (cDNA clone MGC:44873 IMAGE:5528964),
complete cds.
ACCESSION BC032597
VERSION BC032597.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3368)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3368)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
REFERENCE 3 (bases 1 to 3368)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (18-JAN-2018) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK Sequence update by database staff to remove vector contamination
COMMENT On Jan 18, 2018 this sequence version replaced BC032597.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 69 Row: i Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361471.
FEATURES Location/Qualifiers
source 1..3368
/db_xref="H-InvDB:HIT000041625"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:44873 IMAGE:5528964"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3368
/gene="NEDD4L"
/gene_synonym="hNedd4-2"
/gene_synonym="KIAA0439"
/gene_synonym="RSP5"
/db_xref="GeneID:23327"
/db_xref="MIM:606384"
CDS 276..3011
/gene="NEDD4L"
/gene_synonym="hNedd4-2"
/gene_synonym="KIAA0439"
/gene_synonym="RSP5"
/codon_start=1
/product="NEDD4L protein"
/protein_id="AAH32597.1"
/db_xref="GeneID:23327"
/db_xref="MIM:606384"
/translation="MATGLGEPVYGLSEDEGESRILRVKVVSGIDLAKKDIFGASDPY
VKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRD
DFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQD
EENSDQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRT
TQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETI
SEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELSRRLQITPDSNGEQFSSLIQREP
SSRLRSCSVTDAVAEQGHLPPLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSA
PLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPF
FIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFYIDHNSK
ITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEE
SYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSAT
DNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITL
NDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENK
REYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVND
WRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSN
GPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD"
misc_feature 339..599
/gene="NEDD4L"
/gene_synonym="hNedd4-2"
/gene_synonym="KIAA0439"
/gene_synonym="RSP5"
/note="C2; Region: C2 domain"
/db_xref="CDD:pfam00168"
misc_feature 867..947
/gene="NEDD4L"
/gene_synonym="hNedd4-2"
/gene_synonym="KIAA0439"
/gene_synonym="RSP5"
/note="WW; Region: WW domain. The WW domain is a protein
module with two highly conserved tryptophans that binds
proline-rich peptide motifs in vitro"
/db_xref="CDD:pfam00397"
misc_feature 1578..2996
/gene="NEDD4L"
/gene_synonym="hNedd4-2"
/gene_synonym="KIAA0439"
/gene_synonym="RSP5"
/note="HUL4; Region: COG5021, HUL4, Ubiquitin-protein
ligase [Posttranslational modification, protein turnover,
chaperones]"
/db_xref="CDD:COG5021"
BASE COUNT 943 a 843 c 830 g 752 t
ORIGIN
1 ggagaggcgc cggggctgcc gcccggtgct cggcgcgctc tcgggagccg cccgcccgct
61 ggtcccgcag ccttccggga ggaagcggtg ccggcagcgt ccagggcgcg ctctcgggca
121 ccctcacctg ccggcgcccg gccgcttacc cggcagggcg tgcgcagggt agggtgcggg
181 accgggggga cctggaggca gaggggagaa ccggccgtcc gcgccgcagc acagccgctg
241 ggagcgcctc agaccccgcg cggggcgccg gctccatggc gaccgggctc ggggagccgg
301 tctatggact ttccgaagac gagggagagt cccgtattct cagagtaaaa gttgtttctg
361 gaattgatct cgccaaaaag gacatctttg gagccagtga tccgtatgtg aaactttcat
421 tgtacgtagc ggatgagaat agagaacttg ctttggtcca gacaaaaaca attaaaaaga
481 cactgaaccc aaaatggaat gaagaatttt atttcagggt aaacccatct aatcacagac
541 tcctatttga agtatttgac gaaaatagac tgacacgaga cgacttcctg ggccaggtgg
601 acgtgcccct tagtcacctt ccgacagaag atccaaccat ggagcgaccc tatacattta
661 aggactttct cctcagacca agaagtcata agtctcgagt taagggattt ttgcgattga
721 aaatggccta tatgccaaaa aatggaggtc aagatgaaga aaacagtgac cagagggatg
781 acatggagca tggatgggaa gttgttgact caaatgactc ggcttctcag caccaagagg
841 aacttcctcc tcctcctctg cctcccgggt gggaagaaaa agtggacaat ttaggccgaa
901 cttactatgt caaccacaac aaccggacca ctcagtggca cagaccaagc ctgatggacg
961 tgtcctcgga gtcggacaat aacatcagac agatcaacca ggaggcagca caccggcgct
1021 tccgctcccg caggcacatc agcgaagact tggagcccga gccctcggag ggcggggatg
1081 tccccgagcc ttgggagacc atttcagagg aagtgaatat cgctggagac tctctcggtc
1141 tggctctgcc cccaccaccg gcctccccag gatctcggac cagccctcag gagctgtcag
1201 aggaactaag cagaaggctt cagatcactc cagactccaa tggggaacag ttcagctctt
1261 tgattcaaag agaaccctcc tcaaggttga ggtcatgcag tgtcaccgac gcagttgcag
1321 aacagggcca tctaccaccg cttgcagaag atggtgcgtc cggatcagcc acaaacagta
1381 acaaccatct aatcgagcct cagatccgcc ggcctcgtag cctcagctcg ccaacagtaa
1441 ctttatctgc cccgctggag ggtgccaagg actcacccgt acgtcgggct gtgaaagaca
1501 ccctttccaa cccacagtcc ccacagccat caccttacaa ctcccccaaa ccacaacaca
1561 aagtcacaca gagcttcttg ccacccggct gggaaatgag gatagcgcca aacggccggc
1621 ccttcttcat tgatcataac acaaagacta caacctggga agatccacgt ttgaaatttc
1681 cagtacatat gcggtcaaag acatctttaa accccaatga ccttggcccc cttcctcctg
1741 gctgggaaga aagaattcac ttggatggcc gaacgtttta tattgatcat aatagcaaaa
1801 ttactcagtg ggaagaccca agactgcaga acccagctat tactggtccg gctgtccctt
1861 actccagaga atttaagcag aaatatgact acttcaggaa gaaattaaag aaacctgctg
1921 atatccccaa taggtttgaa atgaaacttc acagaaataa catatttgaa gagtcctatc
1981 ggagaattat gtccgtgaaa agaccagatg tcctaaaagc tagactgtgg attgagtttg
2041 aatcagagaa aggtcttgac tatgggggtg tggccagaga atggttcttc ttactgtcca
2101 aagagatgtt caacccctac tacggcctct ttgagtactc tgccacggac aactacaccc
2161 ttcagatcaa ccctaattca ggcctctgta atgaggatca tttgtcctac ttcactttta
2221 ttggaagagt tgctggtctg gccgtatttc atgggaagct cttagatggt ttcttcatta
2281 gaccatttta caagatgatg ttgggaaagc agataaccct gaatgacatg gaatctgtgg
2341 atagtgaata ttacaactct ttgaaatgga tcctggagaa tgaccctact gagctggacc
2401 tcatgttctg catagacgaa gaaaactttg gacagacata tcaagtggat ttgaagccca
2461 atgggtcaga aataatggtc acaaatgaaa acaaaaggga atatatcgac ttagtcatcc
2521 agtggagatt tgtgaacagg gtccagaagc agatgaacgc cttcttggag ggattcacag
2581 aactacttcc tattgatttg attaaaattt ttgatgaaaa tgagctggag ttgctcatgt
2641 gcggcctcgg tgatgtggat gtgaatgact ggagacagca ttctatttac aagaacggct
2701 actgcccaaa ccaccccgtc attcagtggt tctggaaggc tgtgctactc atggacgccg
2761 aaaagcgtat ccggttactg cagtttgtca cagggacatc gcgagtacct atgaatggat
2821 ttgccgaact ttatggttcc aatggtcctc agctgtttac aatagagcaa tggggcagtc
2881 ctgagaaact gcccagagct cacacatgct ttaatcgcct tgacttacct ccatatgaaa
2941 cctttgaaga tttacgagag aaacttctca tggccgtgga aaatgctcaa ggatttgaag
3001 gggtggatta agcaccctgt gcctcggggg tggttgttct tcaagcaagt tctgcttgca
3061 cttttgcatt tgcctaacag acttttgcag aggcgatggc agagagcagc tgcaggcatg
3121 gtccctggag ccgagccttc accacgcact cgtccaagtt cggatgcggg aacctggtcc
3181 cagcttgagt tcctgccttt cccaccacaa attatcaact ggttgatgtg tacactaatt
3241 acatttcagg aggacttaat gctatttatg ttgtgcctct gcaggcaaag cccttaataa
3301 atattttaca tcctttaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3361 aaaaaaaa
//