LOCUS BC032580 1880 bp mRNA linear HUM 26-JUN-2007
DEFINITION Homo sapiens nestin, mRNA (cDNA clone IMAGE:5493839), partial cds.
ACCESSION BC032580
VERSION BC032580.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1880)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1880)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 69 Row: e Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 38176299.
FEATURES Location/Qualifiers
source 1..1880
/db_xref="H-InvDB:HIT000092716"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5493839"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..>1880
/gene="NES"
/gene_synonym="Nbla00170"
/db_xref="GeneID:10763"
/db_xref="HGNC:HGNC:7756"
/db_xref="MIM:600915"
CDS 109..>1880
/gene="NES"
/gene_synonym="Nbla00170"
/codon_start=1
/product="NES protein"
/protein_id="AAH32580.1"
/db_xref="GeneID:10763"
/db_xref="HGNC:HGNC:7756"
/db_xref="MIM:600915"
/translation="MEGCMGEESFQMWELNRRLEAYLARVKALEEQNELLSAELGGLR
AQSADTSWRAHADDELAALRALVDQRWREKHAAEVARDNLAEELEGVAGRCQQLRLAR
ERTTEEVARNRRAVEAEKCARAWLSSQAAELERELEALRVAHEEERVGLNAQAACAPR
CPAPPRGPPAPAPEVEELARRLGEAWRGAVRGYQERVAHMETSLGQARERLGRAVQGA
REGRLELQQLQAERGGLLERRAALEQRLEGRWQERLRATEKFQLAVEALEQEKQGLQS
QIAQVLEGRQQLAHLKMSLSLEVATYRTLLEAENSRLQTPGGGSKTSLSFQDPKLELQ
FPRTPEGRRLGSLLPVLSPTSLPSPLPATLETPVPAFLKNQEFLQARTPTLASTPIPP
TPQAPSPAVDAEIRAQDAPLSLLQTQGGRKQAPEPLRAEARVAIPASVLPGPEEPGGQ
RQEASTGQSPEDHASLAPPLSPDHSSLEAKDGESGGSRVFSICRGEGEGQIWGLVEKE
TAIEGKVVSSLQQEIWEEEDLNRKEIQDSQVPLEKETLKSLGEEIQESLKTLENQSHE
TLERENQECPRSLEEDLETLKKKK"
BASE COUNT 395 a 569 c 636 g 280 t
ORIGIN
1 ccacgcgtcc ggtcggtctc ttttctcttc cgtcctaaaa gctctgcgag ccgctccctt
61 ctcccggtgc cccgcgtctg tccatcctca gtgggtcaga cgagcaggat ggagggctgc
121 atgggggagg agtcgtttca gatgtgggag ctcaatcggc gcctggaggc ctacctggcc
181 cgggtcaagg cgctggagga gcagaatgag ctgctcagcg cggagctcgg ggggctccgg
241 gcacaatccg cggacacctc ctggcgggcg catgccgacg acgagctggc ggccctgcgg
301 gccctcgttg accaacgctg gcgggagaag cacgcggccg aggtggcgcg cgacaacctg
361 gctgaagagc tggagggcgt ggcaggccga tgccagcagc tgcggctggc ccgggagcgg
421 acgacggagg aggtagcccg caaccggcgc gccgtcgagg cagagaaatg cgcccgggcc
481 tggctgagta gccaggcggc agagctggag cgcgagctag aggctctacg cgtggcgcac
541 gaggaggagc gcgtcggcct gaacgcgcag gctgcctgtg ccccccgctg ccccgcgccg
601 ccccgcgggc ctcccgcgcc ggccccggag gtagaggagc tggcaaggcg actgggcgag
661 gcgtggcgcg gggcagtgcg cggctaccag gagcgcgtgg cacacatgga gacgtcgctg
721 ggccaggccc gcgagcggct gggccgggcg gtgcagggtg cccgcgaggg ccgcctggag
781 ctgcagcagc tccaggctga gcgcggaggc ctcctggagc gcagggcagc gttggaacag
841 aggttggagg gccgctggca ggagcggctg cgggctactg aaaagttcca gctggctgtg
901 gaggccctgg agcaggagaa acagggccta cagagccaga tcgctcaggt cctggaaggt
961 cggcagcagc tggcgcacct caagatgtcc ctcagcctgg aggtggccac gtacaggacc
1021 ctcctggagg ctgagaactc ccggctgcaa acacctggcg gtggctccaa gacttccctc
1081 agctttcagg accccaagct ggagctgcaa ttccctagga ccccagaggg ccggcgtctt
1141 ggatctttgc tcccagtcct gagcccaact tccctcccct cacccttgcc tgctaccctt
1201 gagacacctg tgccagcctt tcttaagaac caagaattcc tccaggcccg tacccctacc
1261 ttggccagca cccccatccc ccccacacct caggcaccct ctcctgctgt agatgcagag
1321 atcagagccc aggatgctcc tctctctctg ctccagacac agggtgggag gaaacaggct
1381 ccagagcccc tgcgggctga agccagggtg gccattcctg ccagcgtcct gcctggacca
1441 gaggagcctg ggggccagcg gcaagaggcc agtacaggcc agtccccaga ggaccatgcc
1501 tccttggcac cacccctcag ccctgaccac tccagtttag aggctaagga tggagaatcc
1561 ggtgggtcta gagtgttcag catatgccga ggggaaggtg aagggcaaat ctgggggttg
1621 gtagagaaag aaacagccat agagggcaaa gtggtaagca gcttgcagca ggaaatatgg
1681 gaagaagagg atctaaacag gaaggaaatc caggactccc aggttccttt ggaaaaagaa
1741 accctgaagt ctctgggaga ggagattcaa gagtcactga agactctgga aaaccagagc
1801 catgagacac tagaaaggga gaatcaagaa tgtccgaggt ctttagaaga agacttagaa
1861 acactaaaaa aaaaaaaaaa
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