LOCUS BC032100 2692 bp mRNA linear HUM 23-DEC-2003
DEFINITION Homo sapiens DnaJ (Hsp40) homolog, subfamily A, member 3, mRNA
(cDNA clone MGC:20666 IMAGE:4660602), complete cds.
ACCESSION BC032100
VERSION BC032100.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2692)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2692)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 19, 2003 this sequence version replaced BC032100.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 30 Row: o Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4827025.
FEATURES Location/Qualifiers
source 1..2692
/db_xref="H-InvDB:HIT000041419_03"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20666 IMAGE:4660602"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_15"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2692
/gene="DNAJA3"
/gene_synonym="hTid-1"
/gene_synonym="TID1"
/db_xref="GeneID:9093"
CDS 16..1377
/gene="DNAJA3"
/gene_synonym="hTid-1"
/gene_synonym="TID1"
/codon_start=1
/product="DNAJA3 protein"
/protein_id="AAH32100.1"
/db_xref="GeneID:9093"
/translation="MAARCSTRWLLVVVGTPRLPAISGRGARPPREGVVGAWLSRKLS
VPAFASSLTSCGPRALLTLRPGVSLTGTKHYPFICTASFHTSAPLAKEDYYQILGVPR
NASQKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLSDEVKRKQYDAYGSA
GFDPGASGSQHSYWKGGPTVDPEELFRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFN
QAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCR
RCGGRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQ
KSPVFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKG
IPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGKRS
TGN"
misc_feature 292..489
/gene="DNAJA3"
/gene_synonym="hTid-1"
/gene_synonym="TID1"
/note="DnaJ; Region: DnaJ domain. DnaJ domains (J-domains)
are associated with hsp70 heat-shock system and it is
thought that this domain mediates the interaction.
DnaJ-domain is therefore part of a chaperone (protein
folding) system. The T-antigens are confirmed as DnaJ
containing domains from literature"
/db_xref="CDD:pfam00226"
misc_feature 685..918
/gene="DNAJA3"
/gene_synonym="hTid-1"
/gene_synonym="TID1"
/note="DnaJ_CXXCXGXG; Region: DnaJ central domain (4
repeats). The central cysteine-rich (CR) domain of DnaJ
proteins contains four repeats of the motif CXXCXGXG where
X is any amino acid. The isolated cysteine rich domain
folds in zinc dependent fashion. Each set of two repeats
binds one unit of zinc. Although this domain has been
implicated in substrate binding, no evidence of specific
interaction between the isolated DNAJ cysteine rich domain
and various hydrophobic peptides has been found"
/db_xref="CDD:pfam00684"
misc_feature 934..1299
/gene="DNAJA3"
/gene_synonym="hTid-1"
/gene_synonym="TID1"
/note="DnaJ_C; Region: DnaJ C terminal region. This family
consists of the C terminal region form the DnaJ protein.
Although the function of this region is unknown, it is
always found associated with pfam00226 and pfam00684. DnaJ
is a chaperone associated with the Hsp70 heat-shock system
involved in protein folding and renaturation after stress"
/db_xref="CDD:pfam01556"
BASE COUNT 648 a 693 c 748 g 603 t
ORIGIN
1 tgtccccggg ccaagatggc tgcgcggtgc tccacacgct ggttgctggt ggttgtgggg
61 accccgcggc tgccggctat atcgggtaga ggggcccggc cgcccaggga gggcgtggtg
121 ggggcatggc tgagccgcaa gctgagcgtc cccgcctttg cgtcttccct gacctcttgc
181 ggcccccgag cgctgctgac attgagacct ggtgtcagcc tcacaggaac aaaacattac
241 cctttcattt gtactgcctc cttccacacg agtgcccctt tggccaaaga agattattat
301 cagatattag gagtgcctcg aaatgccagc cagaaagaga tcaagaaagc ctattatcag
361 cttgccaaga agtatcaccc tgacacaaat aaggatgatc ccaaagccaa ggagaagttc
421 tcccagctgg cagaagccta tgaggttttg agtgatgagg tgaagaggaa gcagtacgat
481 gcctacggct ctgcaggctt cgatcctggg gccagcggct cccagcatag ctactggaag
541 ggaggcccca ctgtggaccc cgaggagctg ttcaggaaga tctttggcga gttctcatcc
601 tcttcatttg gagatttcca gaccgtgttt gatcagcctc aggaatactt catggagttg
661 acattcaatc aagctgcaaa gggggtcaac aaggagttca ccgtgaacat catggacacg
721 tgtgagcgct gcaacggcaa ggggaacgag cccggcacca aggtgcagca ttgccactac
781 tgtggcggct ccggcatgga aaccatcaac acaggccctt ttgtgatgcg ttccacgtgt
841 aggagatgtg gtggccgcgg ctccatcatc atatcgccct gtgtggtctg caggggagca
901 ggacaagcca agcagaaaaa gcgagtgatg atccctgtgc ctgcaggagt cgaggatggc
961 cagaccgtga ggatgcctgt gggaaaaagg gaaattttca ttacgttcag ggtgcagaaa
1021 agccctgtgt tccggaggga cggcgcagac atccactccg acctctttat ttctatagct
1081 caggctcttc ttgggggaac agccagagcc cagggcctgt acgagacgat caacgtgacg
1141 atcccccctg ggactcagac agaccagaag attcggatgg gtgggaaagg catcccccgg
1201 attaacagct acggctacgg agaccactac atccacatca agatacgagt tccaaagagg
1261 ctaacgagcc ggcagcagag cctgatcctg agctacgccg aggacgagac agatgtggag
1321 gggacggtga acggcgtcac cctcaccagc tctggaaaaa gatccactgg aaactaggcc
1381 gggaagcagc agcccctcca agggccaggg cacctgggag acgggaggat tccagaacag
1441 cagcactgag ctcccacccg cagagcctct ggacggcctt ggcaacagca aaatcatggg
1501 acaacacctc tctccacgga aaggtcacag tggacagccc gggcagtagg atgcagcccc
1561 agaggctggt ggcagtttcc tgtccattgg taggtgacgg cccctggctc aggcagaggg
1621 agatggttag actcttgcag ggctaaaact ctaatttgga attgaatatt gtggatatct
1681 tagttaaagg ccatgcttac agcttagaaa tgaagcctta agctgcatca agttacgaag
1741 tgattaattt ccttctcagc aaacctccgg gaggttccag aatgagttct tcctgacagg
1801 ttgtcttcac tgggagcgtg gggcccccag gccccaccag caccgtcctc ccctaatgag
1861 gggccctgcc gaggcatcag ctgctctgct cagttagttt ttattcccgg ggtaccaagc
1921 agctgcacag tcggtgcctg ggaggcacgt agaggcccag agagtccctg ggggttctgc
1981 tctgaccgtg tgggtggtga tccttgtcag gatgtacagt cctgcctggg aggcacgtag
2041 aggcccagag agtccctggg ggttctgctc tgaccgtgtg ggtggtgatc cttgtcagga
2101 tgtacagtcc ttgctcccac cccatccagg atggccgcct gtccctgact attgagtcct
2161 gttgttgtaa gccaggcatg gagggctcct gcccttctgc tgagccacag cccattgcag
2221 cactgtgctg gccagacttc agctgccttg ggaactgaag ccctgccact gttgctagtc
2281 aggggcttgg ttctcccact tacactgttg acatctattt tctgaagtgt gtttaaatta
2341 ttcagtgcta atcattgttt tttcctttgt aaatgttgat tcagaaaagg aaagcacagg
2401 ctaagcagtt gaaggttccc caccattcag tgagagcaga acccccattc cccagcctct
2461 gctggtagca tgtcgcagtt tccatgtgtt tcaggatctt cgggctgtcg ttagacaggt
2521 taatgaagaa cacttctcaa cagtttcctt tttgttttcc tttataattc actaaaataa
2581 agcatctatt agtgtctgat ttaggaatgt aaaatgattc tgtattaatg taaataagat
2641 tatctattgc aaaaagatat ttcaaaccta aaaaaaaaaa aaaaaaaaaa aa
//