LOCUS BC031999 1637 bp mRNA linear HUM 28-SEP-2006
DEFINITION Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and
steroid delta-isomerase 1, mRNA (cDNA clone MGC:21365
IMAGE:4755300), complete cds.
ACCESSION BC031999
VERSION BC031999.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1637)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1637)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: James Cleaver, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 28 Row: p Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4504506
This clone grew slowly and was rescued by PCR.
FEATURES Location/Qualifiers
source 1..1637
/db_xref="H-InvDB:HIT000041402"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:21365 IMAGE:4755300"
/tissue_type="Skin, normal"
/clone_lib="NCI_CGAP_Skn3"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6.1"
gene 1..1637
/gene="HSD3B1"
/db_xref="GeneID:3283"
/db_xref="HGNC:HGNC:5217"
/db_xref="MIM:109715"
CDS 88..1209
/gene="HSD3B1"
/codon_start=1
/product="hydroxy-delta-5-steroid dehydrogenase, 3 beta-
and steroid delta-isomerase 1"
/protein_id="AAH31999.1"
/db_xref="GeneID:3283"
/db_xref="HGNC:HGNC:5217"
/db_xref="MIM:109715"
/translation="MTGWSCLVTGAGGFLGQRIIRLLVKEKELKEIRVLDKAFGPELR
EEFSKLQNKTKLTVLEGDILDEPFLKRACQDVSVIIHTACIIDVFGVTHRESIMNVNV
KGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPAPYPHSKK
LAEKAVLAANGWNLKNGGTLYTCALRPMYIYGEGSRFLSASINEALNNNGILSSVGKF
STVNPVYVGNVAWAHILALRALQDPKKAPSIRGQFYYISDDTPHQSYDNLNYTLSKEF
GLRLDSRWSFPLSLMYWIGFLLEIVSFLLRPIYTYRPPFNRHIVTLSNSVFTFSYKKA
QRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKENLKSKTQ"
BASE COUNT 446 a 400 c 383 g 408 t
ORIGIN
1 tgccctctcc agggtcaccc tagaatcaga tctgctcccc agcatcttct gtttcctggt
61 gagtgattcc tgctactttg gatggccatg acgggctgga gctgccttgt gacaggagca
121 ggagggtttc tgggacagag gatcatccgc ctcttggtga aggagaagga gctgaaggag
181 atcagggtct tggacaaggc cttcggacca gaattgagag aggaattttc taaactccag
241 aacaagacca agctgacagt gctggaagga gacattctgg atgagccatt cctgaagaga
301 gcctgccagg acgtctcggt catcatccac accgcctgta tcattgatgt cttcggtgtc
361 actcacagag agtctatcat gaatgtcaat gtgaaaggta cccagctcct gttagaggcc
421 tgtgtccaag ctagtgtgcc agtcttcatc tacaccagta gcatagaggt agccgggccc
481 aactcctaca aggaaatcat ccagaatggc catgaagaag agcctctgga aaacacatgg
541 cccgctccat acccacacag caaaaagctt gctgagaagg ctgtactggc ggctaacggg
601 tggaatctga aaaacggcgg caccctgtac acttgtgcct tacgacccat gtatatctat
661 ggggaaggaa gccgattcct ttctgctagt ataaacgagg ccctgaacaa caatgggatc
721 ctgtcaagtg ttggaaagtt ctccactgtt aacccagtct atgttggcaa tgtggcctgg
781 gcccacattc tggccttgag ggccctgcag gaccccaaga aggccccaag catccgagga
841 cagttctact atatctcaga tgacacgcct caccaaagct atgataacct taattacacc
901 ctgagcaaag agttcggcct ccgccttgat tccagatgga gctttccttt atccctgatg
961 tattggattg gcttcctgct ggaaatagtg agcttcctac tcaggccaat ttacacctat
1021 cgaccgccct tcaaccgcca catagtcaca ttgtcaaata gcgtattcac cttctcttat
1081 aagaaggctc agcgagattt ggcgtataag ccactctaca gctgggagga agccaagcag
1141 aaaacggtgg agtgggttgg ttcccttgtg gaccggcaca aggagaacct gaagtccaag
1201 actcagtgat ttaaggatga cagagatgtg catgtgggta ttgttaggag atgtcatcaa
1261 gctccaccct cctggcctca tacagaaagt gacaagggca caagctcagg tcctgctgcc
1321 tccctttcat acaatggcca acttattgta ttcctcatgt catcaaaacc tgcgcagtca
1381 ttggcccaac aagaaggttt ctgtcctaat catataccag aggaaagacc atgtggtttg
1441 ctgttaccaa atctcagtag ctgattctga acaatttagg gactctttta acttgagggt
1501 cgttttgact actagagctc catttctact cttaaatgag aaaggatttc ctttcttttt
1561 aatcttccat tccttcacat agtttgataa aaagatcaat aaatgtttga atgtttaaaa
1621 aaaaaaaaaa aaaaaaa
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