LOCUS BC031061 2851 bp mRNA linear HUM 11-APR-2007
DEFINITION Homo sapiens minichromosome maintenance complex component 4, mRNA
(cDNA clone MGC:33310 IMAGE:5273457), complete cds.
ACCESSION BC031061
VERSION BC031061.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2851)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2851)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 48 Row: f Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 33469916.
FEATURES Location/Qualifiers
source 1..2851
/db_xref="H-InvDB:HIT000041228"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:33310 IMAGE:5273457"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..2851
/gene="MCM4"
/gene_synonym="CDC54"
/gene_synonym="hCdc21"
/gene_synonym="P1-CDC21"
/db_xref="GeneID:4173"
/db_xref="HGNC:HGNC:6947"
/db_xref="MIM:602638"
CDS 100..2691
/gene="MCM4"
/gene_synonym="CDC54"
/gene_synonym="hCdc21"
/gene_synonym="P1-CDC21"
/codon_start=1
/product="minichromosome maintenance complex component 4"
/protein_id="AAH31061.1"
/db_xref="GeneID:4173"
/db_xref="HGNC:HGNC:6947"
/db_xref="MIM:602638"
/translation="MSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTS
TGELQPMPTSPGVDLQSPAAQDVLFSSPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGT
PRSGVRGTPVRQRPDLGSAQKGLQVDLQSDGAAAEDIVASEQSLGQKLVIWGTDVNVA
ACKENFQRFLQRFIDPLAKEEENVGIDITEPLYMQRLGEINVIGEPFLNVNCEHIKSF
DKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNP
EDIDQLITISGMVIRTSQLIPEMQEAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTT
HSMALIHNRSLFSDKQMIKLQESPEDMPAGQTPHTVILFAHNDLVDKVQPGDRVNVTG
IYRAVPIRVNPRVSNVKSVYKTHIDVIHYRKTDAKRLHGLDEEAEQKLFSEKRVELLK
ELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEINILL
CGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLS
DNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQW
NPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELL
DMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRL
AEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRK
EELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVT
GKTVRLL"
BASE COUNT 770 a 708 c 735 g 638 t
ORIGIN
1 gggagccgac gggaacgtcc gcgctgcgga gcagggcagg gaagccggga ggcgggcccg
61 gcccgagctt gtccttgtcg cgcaggtact ccgagcacta tgtcgtcccc ggcgtcgacc
121 ccgagccgcc gcggcagccg gcgtggaagg gccacccccg cccagacgcc tcggagtgag
181 gatgccaggt catctccctc tcagagacgt agaggcgagg attccacctc cacgggggag
241 ttgcagccga tgccaacctc gcctggagtg gacctgcaga gccctgctgc gcaggacgtg
301 ctgttttcca gccctcccca aatgcattct tcagctatcc ctcttgactt tgatgttagt
361 tcaccactga catacggcac tcccagctct cgggtagagg gaaccccaag aagtggtgtt
421 aggggcacac ctgtgagaca gaggcctgac ctgggctctg cacagaaggg cctgcaagtg
481 gatctgcagt ctgacggggc agcagcagaa gatatagtgg caagtgagca gtctctaggc
541 caaaaacttg tgatctgggg aacagatgta aatgtggcag catgcaaaga aaactttcag
601 agatttcttc agcgttttat tgaccctctg gctaaagaag aagaaaatgt tggcatagat
661 attactgaac ctctatacat gcaacgactt ggggagatta atgttattgg tgagccattt
721 ttaaatgtga actgtgaaca catcaaatca tttgacaaaa atttgtacag acaactcatc
781 tcttacccac aggaagttat tccaactttt gacatggctg tcaatgaaat cttctttgac
841 cgttaccctg actcaatctt agaacatcag attcaagtaa gaccattcaa cgcattgaag
901 actaagaata tgagaaacct gaatccagaa gacattgacc agctcatcac catcagcggc
961 atggtgatca ggacatccca gctgattccc gagatgcagg aggccttctt ccagtgccaa
1021 gtgtgtgccc acacgacccg ggtggagatg gaccgcggcc gcattgcaga gcccagtgtg
1081 tgcgggcgct gccacaccac ccacagcatg gcactcatcc acaaccgctc cctcttctct
1141 gacaagcaga tgatcaagct tcaggagtct ccggaagaca tgcctgcagg gcagacacca
1201 cacacagtta tcctgtttgc tcacaatgat ctcgttgaca aggtccagcc tggggacaga
1261 gtgaatgtta caggcatcta tcgagctgtg cctattcgag tcaatccaag agtgagtaat
1321 gtgaagtctg tctacaaaac ccacattgat gtcattcatt atcggaaaac ggatgcaaaa
1381 cgtctgcatg gccttgatga agaagcagaa cagaaacttt tttcagagaa acgtgtggaa
1441 ttgcttaagg aactttccag gaaaccagac atttatgaga ggcttgcttc agccttggct
1501 ccaagcattt atgaacatga agatataaag aagggaattt tgcttcagct ctttggcggg
1561 acaaggaagg attttagtca cactggaagg ggcaaatttc gggctgagat caacatcttg
1621 ctgtgtggcg accctggtac cagcaagtcc cagctgctgc agtacgtgta caacctcgtc
1681 cccaggggcc agtacacgtc tgggaagggc tccagtgcag ttggcctcac tgcgtacgta
1741 atgaaagacc ctgagacaag gcagctggtc ctgcagacag gtgctcttgt cctgagtgac
1801 aacggcatct gctgtatcga tgagttcgac aagatgaatg aaagtacaag atcggtattg
1861 catgaagtca tggaacagca gactctgtcc attgcaaagg ctgggatcat ctgtcagctc
1921 aatgcgcgca cctctgtcct ggcagcagca aatcccattg agtctcagtg gaatcctaaa
1981 aaaacaacca ttgaaaacat ccagctgcct catactttat tatcaaggtt tgatttgatc
2041 ttcctcatgc tggaccctca ggacgaagcc tatgacaggc gtctggctca ccacctggtc
2101 gcactgtact accagagcga ggagcaggca gaggaggagc tcctggacat ggcggtgcta
2161 aaggactaca ttgcctacgc gcacagcacc atcatgccgc ggctaagtga ggaagccagc
2221 caggctctca tcgaggctta tgtagacatg aggaagattg gcagtagccg gggaatggtt
2281 tctgcatacc ctcgacagct agagtcatta atccgcttag cagaagccca tgctaaagta
2341 agattgtcta acaaagttga agccattgat gtggaagagg ccaaacgcct ccatcgggaa
2401 gctctgaagc agtctgcaac tgatccccgg actggcatcg tggacatatc tattcttact
2461 acggggatga gtgccacctc tcgtaaacgg aaagaagaat tagctgaagc attgaaaaag
2521 cttattttat ctaagggcaa aacaccagct ctaaaatacc agcaactttt tgaagatatt
2581 cggggacaat ctgacatagc aattactaaa gatatgtttg aagaagcact gcgtgccctg
2641 gcagatgatg atttcctgac agtgactggg aagaccgtgc gcttgctctg aagccttgtg
2701 agcaaggaag gctccctgca tgtcctgctt gctgcacgcc acatgggtgt ggtctgcatc
2761 tcagttggcc gccatcagtg taaatagagc ttaaagtcat ggtttggctg cataaaaatt
2821 ttctaacttg ggttcaaaaa aaaaaaaaaa a
//