LOCUS BC030697 1398 bp mRNA linear HUM 19-MAR-2007
DEFINITION Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 2, mRNA (cDNA
clone MGC:26632 IMAGE:4824350), complete cds.
ACCESSION BC030697
VERSION BC030697.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1398)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1398)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (24-MAY-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC030697.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 33 Row: i Column: 2
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 38492354.
FEATURES Location/Qualifiers
source 1..1398
/db_xref="H-InvDB:HIT000041106"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:26632 IMAGE:4824350"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..1398
/gene="PDHA2"
/db_xref="GeneID:5161"
/db_xref="HGNC:HGNC:8807"
/db_xref="MIM:179061"
CDS 60..1226
/gene="PDHA2"
/codon_start=1
/product="pyruvate dehydrogenase (lipoamide) alpha 2"
/protein_id="AAH30697.3"
/db_xref="GeneID:5161"
/db_xref="HGNC:HGNC:8807"
/db_xref="MIM:179061"
/translation="MLAAFISRVLRRVAQKSARRVLVASRNSSNDATFEIKKCDLYLL
EEGPPVTTVLTRAEGLKYYRMMLTVRRMELKADQLYKQKFIRGFCHLCDGQEACCVGL
EAGINPSDHVITSYRAHGVCYTRGLSVRSILAELTGRRGGCAKGKGGSMHMYTKNFYG
GNGIVGAQGPLGAGIALACKYKGNDEICLTLYGDGAANQGQIAEAFNMAALWKLPCVF
ICENNLYGMGTSTERAAASPDYYKRGNFIPGLKVDGMDVLCVREATKFAANYCRSGKG
PILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKRDPIIILQDRMVNSKLATVEELK
EIGAEVRKEIDDAAQFATTDPEPHLEELGHHIYSSDSSFEVRGANPWIKFKSVS"
BASE COUNT 406 a 278 c 365 g 349 t
ORIGIN
1 caggccttcc ggcggtcgtt acgggacgcc gctgccatct acagcactcc gtgaagaata
61 tgctggccgc cttcatctcc cgcgtgttga ggcgagttgc ccagaaatca gctcgcagag
121 tgctggtggc atcccgtaac tcctcaaatg acgctacatt tgaaattaag aaatgtgatc
181 tttatctgtt ggaagagggt ccccctgtca ctacagtgct cactagggcg gaggggctta
241 aatactacag gatgatgctg actgttcgcc gcatggaatt gaaggcagat cagctgtaca
301 aacagaaatt cattcgcggt ttctgtcacc tgtgcgatgg tcaggaagct tgttgcgtgg
361 gccttgaggc cggcataaac ccctcggatc acgtcattac atcctatagg gctcatggtg
421 tgtgctatac tcggggactt tctgtccgat ccattctcgc agagctgacg ggaagaagag
481 gaggttgtgc taaaggaaaa ggaggatcga tgcatatgta taccaagaac ttctatgggg
541 gcaatggcat cgtcggtgca cagggccccc tgggcgctgg cattgctctg gcctgtaaat
601 ataaaggaaa cgatgagatc tgtttgactt tatatgggga tggcgctgcg aatcaggggc
661 agatagccga agctttcaat atggcagctt tatggaaatt accttgtgtt ttcatctgtg
721 agaataacct atatggaatg ggaacatcta ctgagagagc agcagccagc cctgattact
781 acaagagggg caattttatc cctgggctaa aggtcgatgg aatggatgtt ctgtgtgttc
841 gtgaggcaac aaaatttgca gctaactact gtagatctgg aaaggggccc atactgatgg
901 agctgcaaac ctaccgttat catggacaca gtatgagtga tcctggagtc agttatcgta
961 cacgagaaga aattcaggaa gtaagaagta agagggatcc tataataatt ctccaagata
1021 gaatggtaaa cagcaagctc gccactgtgg aagaattaaa ggaaattggg gctgaggtga
1081 ggaaagaaat tgatgatgct gcccagtttg ctaccactga tcctgagcca catttggaag
1141 aattaggcca tcacatctac agcagtgatt catcttttga agttcgtggt gcaaatccat
1201 ggatcaagtt taagtccgtc agttaaaggg aggctacgtg tgaatttatc atcagtctct
1261 caatggaatg ttcatggtca aatttaagaa actgtgttct caactcaagg aggaataaaa
1321 ctcataaaac aaaagccttg taagcattta ttaaaagaga ttattaaaag agattgaagg
1381 acaaaaaaaa aaaaaaaa
//