LOCUS BC030613 3926 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens N-ethylmaleimide-sensitive factor, mRNA (cDNA clone
MGC:32977 IMAGE:4812117), complete cds.
ACCESSION BC030613
VERSION BC030613.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3926)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3926)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Sep 16, 2003 this sequence version replaced BC030613.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 46 Row: d Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 11079227.
FEATURES Location/Qualifiers
source 1..3926
/db_xref="H-InvDB:HIT000041070"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:32977 IMAGE:4812117"
/tissue_type="Brain, hippocampus"
/clone_lib="NIH_MGC_95"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3926
/gene="NSF"
/gene_synonym="SKD2"
/db_xref="GeneID:4905"
/db_xref="HGNC:HGNC:8016"
/db_xref="MIM:601633"
CDS 39..2273
/gene="NSF"
/gene_synonym="SKD2"
/codon_start=1
/product="N-ethylmaleimide-sensitive factor"
/protein_id="AAH30613.1"
/db_xref="GeneID:4905"
/db_xref="HGNC:HGNC:8016"
/db_xref="MIM:601633"
/translation="MAGRSMQAARCPTDELSLTNCAVVNEKDFQSGQHVIVRTSPNHR
YTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFL
QKKSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVKDIEAMDPSIL
KGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKM
GIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKM
LNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAIC
KQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVK
MEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQS
TAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGII
KWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIK
ICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQ
ALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALEL
LGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALLREEGASP
LDFD"
BASE COUNT 1150 a 814 c 881 g 1081 t
ORIGIN
1 gcagagctgc agccgccgag ccggacgtgt ccgcgaagat ggcgggccgg agcatgcaag
61 cggcaagatg tcctacagat gaattatctt taaccaattg tgcagttgtg aatgaaaagg
121 atttccagtc tggccagcat gtgattgtga ggacctctcc caatcacagg tacacattta
181 cactgaagac acatccatcg gtggttccag ggagcattgc attcagttta cctcagagaa
241 aatgggctgg gctttctatt gggcaagaaa tagaagtctc cttatataca tttgacaaag
301 ccaaacagtg tattggcaca atgaccatcg agattgattt cctgcagaaa aaaagcattg
361 actccaaccc ttatgacacc gacaagatgg cagcagaatt tattcagcaa ttcaacaacc
421 aggccttctc agtgggacaa cagcttgtct ttagcttcaa tgaaaagctt tttggcttac
481 tggtgaagga cattgaagcc atggatccta gcatcctgaa gggagagcct gcgacaggga
541 aaaggcagaa gattgaagta ggactggttg ttggaaacag tcaagttgca tttgaaaaag
601 cagaaaattc gtcacttaat cttattggca aagctaaaac caaggaaaat cgccaatcaa
661 ttatcaatcc tgactggaac tttgaaaaaa tgggaatagg aggtctagac aaggaatttt
721 cagatatttt ccgacgagca tttgcttccc gagtatttcc tccagagatt gtggagcaga
781 tgggttgtaa acatgttaaa ggcatcctgt tatatggacc cccaggttgt ggtaagactc
841 tcttggctcg acagattggc aagatgttga atgcaagaga gcccaaagtg gtcaatgggc
901 cagaaatcct taacaaatat gtgggagaat cagaggctaa cattcgcaaa ctttttgctg
961 atgctgaaga ggagcaaagg aggcttggtg ctaacagtgg tttgcacatc atcatctttg
1021 atgaaattga tgccatctgc aagcagagag ggagcatggc tggtagcacg ggagttcatg
1081 acactgttgt caaccagttg ctgtccaaaa ttgatggcgt ggagcagcta aacaacatcc
1141 tagtcattgg aatgaccaat agaccagatc tgatagatga ggctcttctt agacctggaa
1201 gactggaagt taaaatggag ataggcttgc cagatgagaa aggccgacta cagattcttc
1261 acatccacac agcaagaatg agagggcatc agttactctc tgctgatgta gacattaaag
1321 aactggccgt ggagaccaag aatttcagtg gtgctgaatt ggagggtcta gtgcgagcag
1381 cccagtccac tgctatgaat agacacataa aggccagtac taaagtggaa gtggacatgg
1441 agaaagcaga aagcctgcaa gtgacgagag gagacttcct tgcttctttg gagaatgata
1501 tcaaaccagc ctttggcaca aaccaagaag attatgcaag ttacattatg aacggtatca
1561 tcaaatgggg tgacccagtt actcgagttc tagatgatgg ggagctgctg gtgcagcaga
1621 ctaagaacag tgaccgcaca ccattggtca gcgtgcttct ggaaggccct cctcacagtg
1681 ggaagactgc tttagctgca aaaattgcag aggaatccaa cttcccattc atcaagatct
1741 gttctcctga taaaatgatt ggcttttctg aaacagccaa atgtcaggcc atgaagaaga
1801 tctttgatga tgcgtacaaa tcccagctca gttgtgtggt tgtggatgac attgagagat
1861 tgcttgatta cgtccctatt ggccctcgat tttcaaatct tgtattacag gctcttctcg
1921 ttttactgaa aaaggcacct cctcagggcc gcaagcttct tatcattggg accactagcc
1981 gcaaagatgt ccttcaggag atggaaatgc ttaacgcttt cagcaccacc atccacgtgc
2041 ccaacattgc cacaggagag cagctgttgg aagctttgga gcttttgggc aacttcaagg
2101 ataaggaacg caccacaatt gcacagcaag tcaaagggaa gaaggtctgg ataggaatca
2161 agaagttact aatgctgatc gagatgtccc tacagatgga tcctgaatac cgtgtgagaa
2221 aattcttggc cctcttaaga gaagaaggag ctagccccct tgattttgat tgaaaatgaa
2281 ctatttgaaa cacacagtga acaagggaag tgaccaaggt gaagatggcc taggatcttc
2341 actgtcttac tcaagatact ggactaagtg gaacgttctc taccttcaac atgtgctcgc
2401 tctgcatgat tagtgcaata aaactccctt ccttatgcat actgagatag cttagtgtct
2461 cgtggaaggt gtcaatttgg tttagaatgc tgcgcttacc ttcccatgca ggctaaagtg
2521 attccttctt gctcagtccc tctgggtggg aaccatccag tacttgtgga cactacacgt
2581 ttcaacctct ctactagcac catcaccctt gaaaactctc agtcagtgtc atgaatgttg
2641 catgacaaca gttggccgat tagaaggcag actttctaca tgcaaatctg gcttagtaaa
2701 tcgaggtgtg ggccagagat cctctgacag ctgtcctgag ctaacactaa aagtcactgg
2761 gtatttggtt aaaggtctcc cacaagactg gtattctctt tgcctgaaga aacaaggcat
2821 tgaatctcta aaatgctgtt ctcaatcatt gtcagagatg ttttcaagtt gcagtcagaa
2881 gatctttctt aatagaaagt cagatgacta ccgtgttggt tgtgacttcc ccttaagtat
2941 aactaatttg ctctgtggta agagatatgc tcattattac cacttagaag atgttgttaa
3001 aaacatgtga aagataggta tggaaaaagc atacaccccc aaacagaaag gagttattaa
3061 agtaatttac aaacctctca gcactaatta gtgtccaact ccaagtgggt caattcctta
3121 gtataatatt aaggcttact agtatcactg ctttttcctt agcttaatga cttacttaga
3181 atttatcctt tattttaaat gatctgtact atctagtgtc taaaacacta ttctccagaa
3241 aaatcaatca ttttctagcc ctctccctca gtcctttatt gtccattcca atacattgaa
3301 cacatttcct ttaccctcca cacacttctt ccaaaaggaa gcacccgttg agtccttttg
3361 agggtgattt gtcttacaac tgactgactt agcaggaatt taattaggtc atatttggtg
3421 atgagactta tggagtgtgc ctctctctcc caactgctgc ttaaaatgca aggacaagca
3481 attagaagcc atcctaaggt gcttacctca cacgccaccc atgaggcttg tggccacagt
3541 ggcacttggg tgtggctcct ctgttatttg tcctcatgtg agaaagcaga tcatctccaa
3601 atcttgccat ttgtatactt ttggtggaga cttggatgtc atatcttctt tgttttgggt
3661 tttcttccct agcttatttt gtggctttta aagaagtgga ttgtattgtg agatcctgtg
3721 attcctggtg gccagtatcc tggattcctc taagatcttg cctctttcct cctcatgaaa
3781 gcagcacaca ttgtgttaac ttatgtctct tgttaaatga gcttaatgtc tttgtgtttt
3841 gtccaaaact gtattgaaaa aatattgttt aatgcaaatg aaggaatgca ataaagagta
3901 aatatacttg aaaaaaaaaa aaaaaa
//