LOCUS BC030539 1530 bp mRNA linear HUM 22-FEB-2007
DEFINITION Homo sapiens chromosome 21 open reading frame 66, mRNA (cDNA clone
IMAGE:4397726), complete cds.
ACCESSION BC030539
VERSION BC030539.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1530)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1530)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (07-MAY-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 64 Row: a Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 22035568
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..1530
/db_xref="H-InvDB:HIT000041037"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4397726"
/tissue_type="Duodenum, adenocarcinoma"
/clone_lib="NIH_MGC_88"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1530
/gene="C21orf66"
/gene_synonym="BM-020"
/gene_synonym="GCFC"
/db_xref="GeneID:94104"
/db_xref="HGNC:HGNC:13579"
CDS 136..1332
/gene="C21orf66"
/gene_synonym="BM-020"
/gene_synonym="GCFC"
/codon_start=1
/product="C21orf66 protein"
/protein_id="AAH30539.1"
/db_xref="GeneID:94104"
/db_xref="HGNC:HGNC:13579"
/translation="MFRKARRVNVRKRNDSEEEERERDEEQEPPPLLPPPGTGEEAGP
GGGDRAPGGESLLGPGPSPPSALTPGLGAEAGGGFPGGAEPGNGLKPRKRPRENKEVP
RASLLSFQDEEEENEEVFKVKKSSYSKKIVKLLKKEYKEDLEKSKIKTELNSSAESEQ
PLDKTGHVKDTNQEDGVIISEHGEDEMDMESEKEEEKPKTGGAFSNALSSLNVLRPGE
IPDAAFIHAARKKRQMARELGDFTPHDNEPGKGRLVREDENDASDDEDDDEKRRIVFS
VKEKSQRQKIAEEIGIEGSDDDALVTGEQDEELSRWEQEQIRKGINIPQVQASQPAEV
NMYYQNTYQTMPYGSSYGIPYSYTAYGSSDAKSQKTDNTVPFKTPSNEMTPVTIDLVK
KQLKDR"
BASE COUNT 463 a 338 c 437 g 292 t
ORIGIN
1 ccacgcgtcc ggcggtagca acagaggact cgacccggct ggagctccgg agagcgcgcg
61 tgcgccgtca cgagctcggc gctgccgggg ccgcggtgtg gaagcgagta ttcgaccgcc
121 gtgcgggccg cggggatgtt ccgaaaggcc cggcgggtga acgtgcgcaa gcggaacgac
181 tccgaagagg aagagcggga acgcgatgag gagcaggagc cgccgccgtt gttgccgccg
241 ccgggcacgg gcgaagaggc gggccccggt ggcggcgaca gggcccctgg cggggagtcg
301 ctgctgggcc cggggccgtc gccgccttcc gcgctgaccc cgggcctcgg ggctgaggcc
361 gggggcggct tccccggcgg cgcggagccc ggcaacgggc tgaagccgcg caagaggcct
421 cgcgagaaca aagaggtgcc ccgggccagc ctgctcagct tccaggacga ggaggaagaa
481 aatgaagaag ttttcaaagt gaagaaatca agttatagca aaaagatagt aaaattgctc
541 aagaaggaat ataaagaaga tcttgaaaaa tcgaagatta agacagaact caactcatca
601 gctgaaagtg aacaaccttt ggacaaaaca ggacatgtta aggatacaaa tcaagaagat
661 ggagttatca tcagtgaaca tggtgaagat gaaatggata tggaaagtga aaaagaggaa
721 gaaaagccaa agactggtgg agctttttca aatgctttat cttcattgaa tgttcttcgt
781 ccaggagaaa ttccagatgc agcttttata catgctgcaa ggaaaaagcg ccaaatggcc
841 cgagaattgg gagatttcac tcctcatgat aatgagcctg gtaaaggccg ccttgttaga
901 gaagatgaga atgatgccag tgatgatgaa gatgacgatg agaaacgccg gatagttttt
961 tctgtgaaag aaaagtcaca aagacaaaaa attgctgagg aaataggaat tgaggggagt
1021 gatgatgatg ctttagtaac tggagaacag gatgaagagc tcagccgatg ggaacaggag
1081 cagataagga aaggaattaa tatccctcag gttcaagcca gtcaacccgc agaagtgaat
1141 atgtactacc agaacactta ccagacaatg ccttacggct catcctatgg cattccttat
1201 agttatacgg cctatggatc atcagatgcc aaatctcaaa aaacagataa tacagtccct
1261 ttcaaaactc ccagtaatga gatgactccc gttactattg atttggtaaa gaaacagctt
1321 aaagacaggt agggcagatc aacttcttaa agacctgtcc gctagctttc tgttccctca
1381 ggcaacatgt agtctggggt agcagatgca gaatatcagc aaagaagatt cactggccca
1441 gcttcaaaag ctaaacagaa gatgacttca tactttgttc tgctaccttc ttggattact
1501 tctgtggaag cctcgaaaaa aaaaaaaaaa
//