LOCUS BC029128 1455 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens aspartoacylase (Canavan disease), mRNA (cDNA clone
MGC:34517 IMAGE:5180104), complete cds.
ACCESSION BC029128
VERSION BC029128.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1455)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1455)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-MAY-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 50 Row: a Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4557334.
FEATURES Location/Qualifiers
source 1..1455
/db_xref="H-InvDB:HIT000040686"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:34517 IMAGE:5180104"
/tissue_type="Brain, Lung, Testis, adult, pooled whole"
/clone_lib="NIH_MGC_115"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1455
/gene="ASPA"
/gene_synonym="ACY2"
/gene_synonym="ASP"
/db_xref="GeneID:443"
/db_xref="HGNC:HGNC:756"
/db_xref="MIM:608034"
CDS 136..1077
/gene="ASPA"
/gene_synonym="ACY2"
/gene_synonym="ASP"
/codon_start=1
/product="aspartoacylase (Canavan disease)"
/protein_id="AAH29128.1"
/db_xref="GeneID:443"
/db_xref="HGNC:HGNC:756"
/db_xref="MIM:608034"
/translation="MTSCHIAEEHIQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRT
GLEVKPFITNPRAVKKCTRYIDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGP
KDSEDSYDIIFDLHNTTSNMGCTLILEDSRNNFLIQMFHYIKTSLAPLPCYVYLIEHP
SLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPC
AIEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDC
TVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRCCLH"
BASE COUNT 495 a 268 c 260 g 432 t
ORIGIN
1 ctccactcaa gggaattctg tactttgccc tttgggtaaa gtctcattta catttctaaa
61 cctttcttaa gaaaatcgaa tttcctttga tctctcttct gaattgcaga aatcagataa
121 aaactacttg gtgaaatgac ttcttgtcac attgctgaag aacatataca aaaggttgct
181 atctttggag gaacccatgg gaatgagcta accggagtat ttctggttaa gcattggcta
241 gagaatggcg ctgagattca gagaacaggg ctggaggtaa aaccatttat tactaacccc
301 agagcagtga agaagtgtac cagatatatt gactgtgacc tgaatcgcat ttttgacctt
361 gaaaatcttg gcaaaaaaat gtcagaagat ttgccatatg aagtgagaag ggctcaagaa
421 ataaatcatt tatttggtcc aaaagacagt gaagattcct atgacattat ttttgacctt
481 cacaacacca cctctaacat ggggtgcact cttattcttg aggattccag gaataacttt
541 ttaattcaga tgtttcatta cattaagact tctctggctc cactaccctg ctacgtttat
601 ctgattgagc atccttccct caaatatgcg accactcgtt ccatagccaa gtatcctgtg
661 ggtatagaag ttggtcctca gcctcaaggg gttctgagag ctgatatctt ggatcaaatg
721 agaaaaatga ttaaacatgc tcttgatttt atacatcatt tcaatgaagg aaaagaattt
781 cctccctgcg ccattgaggt ctataaaatt atagagaaag ttgattaccc ccgggatgaa
841 aatggagaaa ttgctgctat catccatcct aatctgcagg atcaagactg gaaaccactg
901 catcctgggg atcccatgtt tttaactctt gatgggaaga cgatcccact gggcggagac
961 tgtaccgtgt accccgtgtt tgtgaatgag gccgcatatt acgaaaagaa agaagctttt
1021 gcaaagacaa ctaaactaac gctcaatgca aaaagtattc gctgctgttt acattagaaa
1081 tcacttccag cttacatctt acacggtgtc ttacaaattc tgctagtctg taagctcctt
1141 aagagtaggg ttgtgcctta ttcaactgca tacatagctc ctagcacagt gccttattcg
1201 gtaggcatct aagcaaattt cttaaattaa ttaatatatc tttaaagata tcatatttta
1261 tgtatgtagc ttattcaaag aagtgtttcc tatttctata tagtttatta tacatgatac
1321 ttgggtagct caacattctt aataaacagc ctttgtattc agaatataaa attgaaatag
1381 atatatataa agttaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1441 aaaaaaaaaa aaaaa
//