LOCUS BC028235 2453 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens carbohydrate (keratan sulfate Gal-6) sulfotransferase
1, mRNA (cDNA clone MGC:40025 IMAGE:5245338), complete cds.
ACCESSION BC028235
VERSION BC028235.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2453)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2453)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (08-APR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 62 Row: n Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 31542307.
FEATURES Location/Qualifiers
source 1..2453
/db_xref="H-InvDB:HIT000040504"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:40025 IMAGE:5245338"
/tissue_type="Brain, fetal, whole pooled"
/clone_lib="NIH_MGC_121"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2453
/gene="CHST1"
/gene_synonym="C6ST"
/gene_synonym="KS6ST"
/gene_synonym="KSGal6ST"
/db_xref="GeneID:8534"
/db_xref="HGNC:HGNC:1969"
/db_xref="MIM:603797"
CDS 340..1575
/gene="CHST1"
/gene_synonym="C6ST"
/gene_synonym="KS6ST"
/gene_synonym="KSGal6ST"
/codon_start=1
/product="carbohydrate (keratan sulfate Gal-6)
sulfotransferase 1"
/protein_id="AAH28235.1"
/db_xref="GeneID:8534"
/db_xref="HGNC:HGNC:1969"
/db_xref="MIM:603797"
/translation="MQCSWKAVLLLALASIAIQYTAIRTFTAKSFHTCPGLAEAGLAE
RLCEESPTFAYNLSRKTHILILATTRSGSSFVGQLFNQHLDVFYLFEPLYHVQNTLIP
RFTQGKSPADRRVMLGASRDLLRSLYDCDLYFLENYIKPPPVNHTTDRIFRRGASRVL
CSRPVCDPPGPADLVLEEGDCVRKCGLLNLTVAAEACRERSHVAIKTVRVPEVNDLRA
LVEDPRLNLKVIQLVRDPRGILASRSETFRDTYRLWRLWYGTGRKPYNLDVTQLTTVC
EDFSNSVSTGLMRPPWLKGKYMLVRYEDLARNPMKKTEEIYGFLGIPLDSHVARWIQN
NTRGDPTLGKHKYGTVRNSAATAEKWRFRLSYDIVAFAQNACQQVLAQLGYKIAASEE
ELKNPSVSLVEERDFRPFS"
BASE COUNT 500 a 799 c 670 g 484 t
ORIGIN
1 gccgcggctg ctgccgccgc cgccacccgc gggtccccgg cgaccctgac tccagacccg
61 aggatggagc cggcgctggg cgctgcagct gctcccggcg cgtccccgac caggtagctg
121 gtgtcacttc ggtgtggttg gaagaagact ttctccccag ctgcattccc ggaggcgccc
181 tttcgacctg gaggccgggt ctgctggcca cagggctgcc gcactggctg ggactgccag
241 ctgggcctgg agacgctggt ggctgtggac tccccagctt ggagcagtcc ctctttgacc
301 tcaccccttg gagaagcagc cccatgaagg tgcccagcca tgcaatgttc ctggaaggcc
361 gtcctcctcc ttgccctggc ctccattgcc atccagtaca cggccatccg caccttcacc
421 gccaagtcct ttcacacctg ccccgggctg gcagaggccg ggctggccga gcgactgtgc
481 gaggagagcc ccaccttcgc ctacaacctc tcccgcaaga cccacatcct catcctggcc
541 accacgcgca gcggctcctc cttcgtgggc cagctcttca accagcacct ggacgtcttc
601 tacctgtttg agcccctcta ccacgtccag aacacgctca tcccccgctt cacccagggc
661 aagagcccgg ccgaccggcg ggtcatgcta ggcgccagcc gcgacctcct gcggagcctc
721 tacgactgcg acctctactt cctggagaac tacatcaagc cgccgccggt caaccacacc
781 accgacagga tcttccgccg cggggccagc cgggtcctct gctcccggcc tgtgtgcgac
841 cctccggggc cagccgacct ggtcctggag gagggggact gtgtgcgcaa gtgcgggcta
901 ctcaacctga ccgtggcggc cgaggcgtgc cgcgagcgca gccacgtggc catcaagacg
961 gtgcgcgtgc ccgaggtgaa cgacctgcgc gccctggtgg aagacccgcg attaaacctc
1021 aaggtcatcc agctggtccg agacccccgc ggcattctgg cttcgcgcag cgagaccttc
1081 cgcgacacgt accggctctg gcggctctgg tacggcaccg ggaggaaacc ctacaacctg
1141 gacgtgacgc agctgaccac ggtgtgcgag gacttctcca actccgtgtc caccggcctc
1201 atgcggcccc cgtggctcaa gggcaagtac atgttggtgc gctacgagga cctggctcgg
1261 aaccctatga agaagaccga ggagatctac gggttcctgg gcatcccgct ggacagccac
1321 gtggcccgct ggatccagaa caacacgcgg ggcgacccca ccctgggcaa gcacaaatac
1381 ggcaccgtgc gaaactcggc ggccacggcc gagaagtggc gcttccgcct ctcctacgac
1441 atcgtggcct ttgcccagaa cgcctgccag caggtgctgg cccagctggg ctacaagatc
1501 gccgcctcgg aggaggagct gaagaacccc tcggtcagcc tggtggagga gcgggacttc
1561 cgccccttct cgtgacccgg gcggtgcggg tgggggcggg aggcgcaagg tgtcggtttt
1621 gataaaatgg accgttttta actgttgcct tattaacccc tccctctccc acctcatctt
1681 cgtgtccttc ctgcccccag ctcaccccac tcccttctgc cccttttttg tctctgaaat
1741 ttgcactacg tcttggacgg gaatcactgg ggcagagggc gcctgaagta gggtcccgcc
1801 ccccccaccc cattcagaca catggatgtt gggtctctgt gcggacggtg acaatgttta
1861 caagcaccac atttacacat ccacacacgc acacgggcac tcgcgaggcg acttctcaag
1921 cttttgaatg ggtgagtggt cgggtatcta gtttttgcac tgtcttacta ttcaaggtaa
1981 gaggatacaa acaagaggac cacttgtctc taatttatga atggtgtcca tcctttcccc
2041 atccctgcct cctgcccctg acgcccattt ccccccttag agcagcgaaa ctgccccctc
2101 ctgcccgccc ttgcctgtcg gtgaggcagg tttttactgt gaggtgaacg tggacctgtt
2161 tctgtttcca gtctgtggtg atgctgtctg tctgtctgag tctcgtggcc gcccctggac
2221 cagtgatgac tgatgaatct tatgagcctc tgattgatct cggggtccat ctgtgatatt
2281 tctttgtgcc aaaaagaaaa aaaaagagtg gatcagtttg ctaaatgaac attgaaattg
2341 aaatgcttta tctgtgtttt ctgtaaataa aagagtgcaa taatcaaaaa aaaaaaaaaa
2401 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
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