LOCUS BC028058 1849 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens UDP-GlcNAc:betaGal
beta-1,3-N-acetylglucosaminyltransferase 5, mRNA (cDNA clone
MGC:39847 IMAGE:5175670), complete cds.
ACCESSION BC028058
VERSION BC028058.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1849)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1849)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (08-APR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 62 Row: e Column: 2
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 44680131
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..1849
/db_xref="H-InvDB:HIT000050934"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:39847 IMAGE:5175670"
/tissue_type="Brain, Lung, Testis, adult, pooled whole"
/clone_lib="NIH_MGC_115"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1849
/gene="B3GNT5"
/gene_synonym="B3GN-T5"
/gene_synonym="beta3Gn-T5"
/db_xref="GeneID:84002"
/db_xref="HGNC:HGNC:15684"
CDS 524..1660
/gene="B3GNT5"
/gene_synonym="B3GN-T5"
/gene_synonym="beta3Gn-T5"
/codon_start=1
/product="B3GNT5 protein"
/protein_id="AAH28058.1"
/db_xref="GeneID:84002"
/db_xref="HGNC:HGNC:15684"
/translation="MRMLVSGRRVKKWQLIIQLFATCFLASLMFFWEPIDNHIVSHMK
SYSYRYLINSYDFVNDTLSLKHTSAGPRYQYLINHKEKCQAQDVLLLLFVKTAPENYD
RRSGIRRTWGNENYVRSQLNANIKTLFALGTPNPLEGEELQRKLAWEDQRYNDIIQQD
FVDSFYNLTLKLLMQFSWANTYCPHAKFLMTADDDIFIHMPNLIEYLQSLEQIGVQDF
WIGRVHRGAPPIRDKSSKYYVSYEMYQWPAYPDYTAGAAYVISGDVAAKVYEASQTLN
SSLYIDDVFMGLCANKIGIVPQDHVFFSGEGKTPYHPCIYEKMMTSHGHLEDLQDLWK
NATDPKVKTISKGFFGQIYCRLMKIILLCKISYVDTYPCRAAFI"
BASE COUNT 606 a 366 c 370 g 507 t
ORIGIN
1 tgcacccaca gcagtctgtt gtggatggtt gctgagctgc gcattcggca ttgggcttgc
61 tttgtttcct gccaggccca gcattttctt ctaccagatc ggcaggcttg tgggcttctt
121 cctaggtccc tcccctgcac tctgaatagg aaagctggaa gctgtgcttt agagaagctt
181 taagacgccg aaagaaacca gaagagtgag cgccagttgt atgtggcatg gaatattcac
241 atgggagagc cgcatgaggc cgcccaccac gcttcctgaa ggatgcccgt gtggaagaat
301 tttgacgtgc cagtgtcctc gttctacagg gtgttccatt cttccgcaat ctcagaaaaa
361 tgggactaaa agaaactatt ttgtaaaata agaagacttc catttttaat gaccaacatg
421 tattaagatg gacacctact ctacgaaaca caaagttcta tggtctcgaa gaagcccgtg
481 cctgtttaaa actgatccta actaaaaaca gacttgagtg gatatgagaa tgttggttag
541 tggcagaaga gtcaaaaaat ggcagttaat tattcagtta tttgctactt gttttttagc
601 gagcctcatg tttttttggg aaccaatcga taatcacatt gtgagccata tgaagtcata
661 ttcttacaga tacctcataa atagctatga ctttgtgaat gataccctgt ctcttaagca
721 cacctcagcg gggcctcgct accaatactt gattaaccac aaggaaaagt gtcaagctca
781 agacgtcctc cttttactgt ttgtaaaaac tgctcctgaa aactatgatc gacgttccgg
841 aattagaagg acgtggggca atgaaaatta tgttcggtct cagctgaatg ccaacatcaa
901 aactctgttt gccttaggaa ctcctaatcc actggaggga gaagaactac aaagaaaact
961 ggcttgggaa gatcaaaggt acaatgatat aattcagcaa gactttgttg attctttcta
1021 caatcttact ctgaaattac ttatgcagtt cagttgggca aatacctatt gtccacatgc
1081 caaatttctt atgactgctg atgatgacat atttattcac atgccaaatc tgattgagta
1141 ccttcaaagt ttagaacaaa ttggtgttca agacttttgg attggtcgtg ttcatcgtgg
1201 tgcccctccc attagagata aaagcagcaa atactacgtg tcctatgaaa tgtaccagtg
1261 gccagcttac cctgactaca cagccggagc tgcctatgta atctccggtg atgtagctgc
1321 caaagtctat gaggcatcac agacactaaa ttcaagtctt tacatagacg atgtgttcat
1381 gggcctctgt gccaataaaa tagggatagt accgcaggac catgtgtttt tttctggaga
1441 gggtaaaact ccttatcatc cctgcatcta tgaaaaaatg atgacatctc atggacactt
1501 agaagatctc caggaccttt ggaagaatgc tacagatcct aaagtaaaaa ccatttccaa
1561 aggttttttt ggtcaaatat actgcagatt aatgaagata attctccttt gtaaaattag
1621 ctatgtggac acataccctt gtagggctgc gtttatctaa tagtacttga atgttgtatg
1681 ttttcactgt cactgagtca aacctggatg aaaaaaacct ttaaatgttc gtctataccc
1741 taagtaaaat gaggacgaaa gacaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1801 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
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