LOCUS BC027991 1469 bp mRNA linear HUM 27-OCT-2006
DEFINITION Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate
methyltransferase, mRNA (cDNA clone MGC:39926 IMAGE:5000797),
complete cds.
ACCESSION BC027991
VERSION BC027991.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1469)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1469)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (08-APR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 62 Row: c Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 56699398.
FEATURES Location/Qualifiers
source 1..1469
/db_xref="H-InvDB:HIT000091433"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:39926 IMAGE:5000797"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_10"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1469
/gene="TRMU"
/gene_synonym="MTO2"
/gene_synonym="MTU1"
/gene_synonym="TRMT1"
/gene_synonym="TRNT1"
/db_xref="GeneID:55687"
/db_xref="HGNC:HGNC:25481"
/db_xref="MIM:610230"
CDS 319..1122
/gene="TRMU"
/gene_synonym="MTO2"
/gene_synonym="MTU1"
/gene_synonym="TRMT1"
/gene_synonym="TRNT1"
/codon_start=1
/product="TRMU protein"
/protein_id="AAH27991.1"
/db_xref="GeneID:55687"
/db_xref="HGNC:HGNC:25481"
/db_xref="MIM:610230"
/translation="MKKSLSRSTLRSPKGFSEIGLKLEMVSQDALRRTIFPLGGLTKE
FVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTH
KGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIA
EEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFY
KGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL"
BASE COUNT 343 a 366 c 469 g 291 t
ORIGIN
1 ccacgcgtcc ggcgactggc ggatgcaggc cttgcggcac gtcgtgtgcg ccctgtccgg
61 cggcgtggac agcgccgtgg ccgcgctgct gctgaggcgg agaggttacc aggtgacagg
121 ggtgtttatg aagaactggg actcactgga tgaacatggg gtctgtactg ccgacaaaga
181 ctgtgaagat gcttacagag tttgccagat cttagacatc cctttccatc aagtgtccta
241 cgtaaaggag tattggaatg atgtgttcag gggcagatgc cattgccaca ggtcactatg
301 caagaacttc cctggaagat gaagaagtct ttgagcagaa gcacgttaag aagcccgaag
361 ggcttttcag aaatcggttt gaagttagaa atggtttccc aggatgccct gaggagaacc
421 atcttccctc tggggggatt aacgaaagag tttgtaaaga aaatcgctgc tgagaataga
481 cttcatcatg tgcttcagaa gaaagagagc atgggcatgt gtttcatcgg gaagaggaat
541 tttgaacatt tccttcttca gtatctgcag cctcgacctg gtcactttat ttccatagaa
601 gacaataagg ttctgggaac acataaaggt tggttcctgt ataccttggg ccagagagca
661 aacataggtg gcctgagaga gccctggtac gtggtggaga aggacagcgt caagggtgac
721 gtgtttgtgg ccccccggac agaccaccca gccctgtaca gggacctgct gaggaccagc
781 cgcgtgcact ggattgcgga ggagcctccc gcagcactgg tccgggacaa gatgatggag
841 tgccacttcc gattccgcca ccagatggca ctagtgccct gtgtgctgac cctcaatcaa
901 gatggcaccg tgtgggtgac agctgtgcag gctgtgcgtg cccttgccac aggacagttt
961 gctgtgttct acaaggggga cgagtgcctg ggcagcggga agatcctgcg gctggggccg
1021 tctgcctaca cgctccagaa gggccagcgc agagctggga tggccactga gagccccagt
1081 gacagcccag aagatggtcc aggcctgagt cccttgctct gacagagatg gatctgctag
1141 aaggaacctg gagagcagga cccatggctg ggcggctggt gagcagtcca ggtgcccaag
1201 ggccagcttg ctgctgccca aagcagagga agccgggctg gctgagggtc cgaaaagcct
1261 gcaggggccc ggcgagcccc aggaagagcc tcagctccag gctggggctc tggctgctgg
1321 agcatctgct ggctggtggg gtggcccgag ttccccttca ccgcccccag ggagggtttc
1381 ccacctcaga gtacaccaag gggacctgca gagggggctg tcgggacagc gtggaataaa
1441 cattatttca aggaaaaaaa aaaaaaaaa
//