LOCUS BC026246 2306 bp mRNA linear HUM 06-APR-2004
DEFINITION Homo sapiens alpha-2-macroglobulin, mRNA (cDNA clone
IMAGE:4733707), complete cds.
ACCESSION BC026246
VERSION BC026246.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2306)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2306)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (02-APR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 37 Row: i Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 6226959
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..2306
/db_xref="H-InvDB:HIT000258991"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4733707"
/tissue_type="Liver"
/clone_lib="NIH_MGC_76"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..2306
/gene="A2M"
/db_xref="GeneID:2"
/db_xref="MIM:103950"
CDS 70..1131
/gene="A2M"
/codon_start=1
/product="A2M protein"
/protein_id="AAH26246.1"
/db_xref="GeneID:2"
/db_xref="MIM:103950"
/translation="MGKNKLLHPSLVLLLLVLLPTDASVSGKPQYMVLVPSLLHTETT
EKGCVLLSYLNETVTVSASLESVRGNRSLFTDLEAENDVLHCVAFAVPKSSSNEEVMF
LTVQVKGPTQEFKKRTTVMVKNEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNE
LIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHP
FTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDAS
DCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVE
LTGRQSSEITRTITKACCS"
misc_feature 130..1116
/gene="A2M"
/note="A2M_N; Region: Alpha-2-macroglobulin family
N-terminal region. This family includes the N-terminal
region of the alpha-2-macroglobulin family"
/db_xref="CDD:pfam01835"
BASE COUNT 658 a 547 c 544 g 557 t
ORIGIN
1 ggaccagatg gattgtaggg agtagggtac aatacagtct gttctcctcc agctccttct
61 ttctgcaaca tggggaagaa caaactcctt catccaagtc tggttcttct cctcttggtc
121 ctcctgccca cagacgcctc agtctctgga aaaccgcagt atatggttct ggtcccctcc
181 ctgctccaca ctgagaccac tgagaagggc tgtgtccttc tgagctacct gaatgagaca
241 gtgactgtaa gtgcttcctt ggagtctgtc aggggaaaca ggagcctctt cactgacctg
301 gaggcggaga atgacgtact ccactgtgtc gccttcgctg tcccaaagtc ttcatccaat
361 gaggaggtaa tgttcctcac tgtccaagtg aaaggaccaa cccaagaatt taagaagcgg
421 accacagtga tggttaagaa cgaggacagt ctggtctttg tccagacaga caaatcaatc
481 tacaaaccag ggcagacagt gaaatttcgt gttgtctcca tggatgaaaa ctttcacccc
541 ctgaatgagt tgattccact agtatacatt caggatccca aaggaaatcg catcgcacaa
601 tggcagagtt tccagttaga gggtggcctc aagcaatttt cttttcccct ctcatcagag
661 cccttccagg gctcctacaa ggtggtggta cagaagaaat caggtggaag gacagagcac
721 cctttcaccg tggaggaatt tgttcttccc aagtttgaag tacaagtaac agtgccaaag
781 ataatcacca tcttggaaga agagatgaat gtatcagtgt gtggcctata cacatatggg
841 aagcctgtcc ctggacatgt gactgtgagc atttgcagaa agtatagtga cgcttccgac
901 tgccacggtg aagattcaca ggctttctgt gagaaattca gtggacagct aaacagccat
961 ggctgcttct atcagcaagt aaaaaccaag gtcttccagc tgaagaggaa ggagtatgaa
1021 atgaaacttc acactgaggc ccagatccaa gaagaaggaa cagtggtgga attgactgga
1081 aggcagtcca gtgaaatcac aagaaccata accaaagcgt gctgctcatg aagcctgatg
1141 ctgagctctc ggcgtcctcg gtttacaacc tgctaccaga aaaggacctc actggcttcc
1201 ctgggccttt gaatgaccag gacgatgaag actgcatcaa tcgtcataat gtctcatgaa
1261 ctaccctgtg gccatactca gacagtccag gcacattata ttctgaatgg aggcaccctg
1321 ctggggctga agaagctctc cttctattat ctgataatgg caaagggagg cattgtccga
1381 actgggactc atggactgct tgtgaagcag gaagacatga agggccattt ttccatctca
1441 atccctgtga agtcagacat tgctcctgtc gctcggttgc tcatctatgc tgttttacct
1501 accggggacg tgattgggga ttctgcaaaa tatggagcag ccacatttac caggactggg
1561 aaggctgcac aggtgactat ccagtcttca gggacatttt ccagcaaatt ccaagtggac
1621 aacaacaacc gcctgttact gcagcaggtc tcattgccag agctgcctgg ggaatacagc
1681 atgaaagtga caggagaagg atgtgtctac ctccagacat ccttgaaata caatattctc
1741 ccagaaaagg aagagttccc ctttgcttta ggagtgcaga ctctgcctca aacttgtgat
1801 gaacccaaag cccacaccag cttccaaatc tccctaagtg tcagttacac agggagccgc
1861 tctgcctcca acatggcgat cgttgatgtg aagatggtct ctggcttcat tcccctgaag
1921 ccaacagtga agatgcttga aagatctaac catgtgagcc ggacagaagt cagcagcaac
1981 catgtcttga tttaccttga taaggtgtca aatcagacac tgagcttgtt cttcacggtt
2041 ctgcaagatg tcccagtaag agatctgaaa ccagccatag tgaaagtcta tgattactac
2101 gagacggatg agtttgcaat tgctgagtac aatgctcctt gcagcaaaga tcttggaaat
2161 gcttgaagac cacaaggctg aaaagtgctt tgctggagtc ctgttctcag agctccacag
2221 aagacacgtg tttttgtatc tttaaagact tgatgaataa acactttttc tggtcaaaaa
2281 aaaaaaaaaa aaaaaaaaaa aaaaaa
//