LOCUS BC026163 2361 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens sperm adhesion molecule 1 (PH-20 hyaluronidase, zona
pellucida binding), mRNA (cDNA clone MGC:26532 IMAGE:4838230),
complete cds.
ACCESSION BC026163
VERSION BC026163.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2361)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2361)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-APR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 34 Row: n Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 23510416.
FEATURES Location/Qualifiers
source 1..2361
/db_xref="H-InvDB:HIT000040121"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:26532 IMAGE:4838230"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..2361
/gene="SPAM1"
/gene_synonym="HYA1"
/gene_synonym="HYAL1"
/gene_synonym="HYAL3"
/gene_synonym="MGC26532"
/gene_synonym="PH-20"
/gene_synonym="PH20"
/gene_synonym="SPAG15"
/db_xref="GeneID:6677"
/db_xref="HGNC:HGNC:11217"
/db_xref="MIM:600930"
CDS 363..1898
/gene="SPAM1"
/gene_synonym="HYA1"
/gene_synonym="HYAL1"
/gene_synonym="HYAL3"
/gene_synonym="MGC26532"
/gene_synonym="PH-20"
/gene_synonym="PH20"
/gene_synonym="SPAG15"
/codon_start=1
/product="sperm adhesion molecule 1 (PH-20 hyaluronidase,
zona pellucida binding)"
/protein_id="AAH26163.1"
/db_xref="GeneID:6677"
/db_xref="HGNC:HGNC:11217"
/db_xref="MIM:600930"
/translation="MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVI
PNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYI
DSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKP
KDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGY
YLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATL
YVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGAS
GIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNW
NSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVK
DTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIWRLEVWDQGISRI
GFF"
BASE COUNT 756 a 441 c 467 g 697 t
ORIGIN
1 agcggaggtg gttagcagca cctcataagg tccttcctag caagggatgc taatgactag
61 ccaatgctct aggaagacat tgagaccagc caacttcttg ccttgataac tactgaagag
121 acattgggtg gctggatttt gaaagcagac ttctggttat aggtgatgca acttgaaaaa
181 caatcctgaa acatgaaaca agaataataa tatttaaatg taacttaatc attatacctc
241 tttatccatc aaagtgaatt cattccattc cctttcatct gtgctcatac tttgcatcag
301 atattgggta aaccaaagtg tgtaggaaga aataaatgtt ttcatagtca ttactcttta
361 caatgggagt gctaaaattc aagcacatct ttttcagaag ctttgttaaa tcaagtggag
421 tatcccagat agttttcacc ttccttctga ttccatgttg cttgactctg aatttcagag
481 cacctcctgt tattccaaat gtgcctttcc tctgggcctg gaatgcccca agtgaatttt
541 gtcttggaaa atttgatgag ccactagata tgagcctctt ctctttcata ggaagccccc
601 gaataaacgc caccgggcaa ggtgttacaa tattttatgt tgatagactt ggctactatc
661 cttacataga ttcaatcaca ggagtaactg tgaatggagg aatcccccag aagatttcct
721 tacaagacca tctggacaaa gctaagaaag acattacatt ttatatgcca gtagacaatt
781 tgggaatggc tgttattgac tgggaagaat ggagacccac ttgggcaaga aactggaaac
841 ctaaagatgt ttacaagaat aggtctattg aattggttca gcaacaaaat gtacaactta
901 gtctcacaga ggccactgag aaagcaaaac aagaatttga aaaagcaggg aaggatttcc
961 tggtagagac tataaaattg ggaaaattac ttcggccaaa tcacttgtgg ggttattatc
1021 tttttccgga ttgttacaac catcactata agaaacccgg ttacaatgga agttgcttca
1081 atgtagaaat aaaaagaaat gatgatctca gctggttgtg gaatgaaagc actgctcttt
1141 acccatccat ttatttgaac actcagcagt ctcctgtagc tgctacactc tatgtgcgca
1201 atcgagttcg ggaagccatc agagtttcca aaatacctga tgcaaaaagt ccacttccgg
1261 tttttgcata tacccgcata gtttttactg atcaagtttt gaaattcctt tctcaagatg
1321 aacttgtgta tacatttggc gaaactgttg ctctgggtgc ttctggaatt gtaatatggg
1381 gaaccctcag tataatgcga agtatgaaat cttgcttgct cctagacaat tacatggaga
1441 ctatactgaa tccttacata atcaacgtca cactagcagc caaaatgtgt agccaagtgc
1501 tttgccagga gcaaggagtg tgtataagga aaaactggaa ttcaagtgac tatcttcacc
1561 tcaacccaga taattttgct attcaacttg agaaaggtgg aaagttcaca gtacgtggaa
1621 aaccgacact tgaagacctg gagcaatttt ctgaaaaatt ttattgcagc tgttatagca
1681 ccttgagttg taaggagaaa gctgatgtaa aagacactga tgctgttgat gtgtgtattg
1741 ctgatggtgt ctgtatagat gcttttctaa aacctcccat ggagacagaa gaacctcaaa
1801 ttttctacaa tgcttcaccc tccacactat ctgccacaat gttcatttgg aggctggaag
1861 tctgggatca aggtattagc agaattggtt tcttctgaga gtcatgaggg aaaaatgtgt
1921 ttcaggcctc ttcccttggc ttacaggaaa tgaaaaaacc atgactatca tcaccaacat
1981 ccttgggtat taagtgcagt cactctccta gatgctgtgg ggagaaggca agttacaaag
2041 atagaccttc cctcaagata atcagatttt catggtatta tccttaacct ttttgacatc
2101 atggaggctt tgggaatctg atgaagccta tcaattttct tccagaagat atttatataa
2161 gattataaga aaaattatgt acacagctta ttttattgca ttggatcaaa atgccattta
2221 taaagaatta tgccttttcc atcaatttta gcatggaaaa ataatttcag gcaatatgct
2281 taaaaattgg gggaagacaa aagaaatcca tatcgtgtaa ataaaaataa attttggttt
2341 tgctcaaaaa aaaaaaaaaa a
//