LOCUS BC025700 1753 bp mRNA linear HUM 02-JUL-2005
DEFINITION Homo sapiens AF4/FMR2 family, member 4, mRNA (cDNA clone
IMAGE:5221893), complete cds.
ACCESSION BC025700
VERSION BC025700.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1753)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1753)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-MAR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 49 Row: k Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7656878
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..1753
/db_xref="H-InvDB:HIT000039968"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5221893"
/tissue_type="Pancreas, Spleen, adult pooled"
/clone_lib="NIH_MGC_120"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1753
/gene="AFF4"
/gene_synonym="AF5Q31"
/gene_synonym="MCEF"
/db_xref="GeneID:27125"
/db_xref="MIM:604417"
CDS 261..1358
/gene="AFF4"
/gene_synonym="AF5Q31"
/gene_synonym="MCEF"
/codon_start=1
/product="AFF4 protein"
/protein_id="AAH25700.1"
/db_xref="GeneID:27125"
/db_xref="MIM:604417"
/translation="MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSK
EDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGS
HQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSG
SSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDA
NWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYS
SQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAI
HTPCKTEPSKFPFPTKESQQSNFGTGEQRLK"
BASE COUNT 531 a 458 c 398 g 366 t
ORIGIN
1 cccgggcccg accgtcatgg agcggtgcgg ggcctacgcc tgttccgtgt gaggcgcatc
61 gctgccgccg ccgccgacgc cgccttcgtt tgtcgaggcc tccggaaccc ccgccgcctc
121 ccggagcccc tcgcccttgg cagcctgtca ccgccccctg ggcgggctcg aatgcgcgtc
181 cggtgaaggt gcaggcccgg cgccgccact gccgcagcca ggagatggtt cgggcctagc
241 ggagccggga ctggagcaac atgaaccgtg aagaccggaa tgtgctgcgt atgaaagaac
301 gggaaaggcg gaatcaggaa attcagcagg gcgaagacgc cttcccacct agctctcctc
361 tctttgcaga gccatacaaa gttactagca aagaagataa gttatcaagt cgtattcaga
421 gtatgcttgg aaactacgat gaaatgaagg atttcatagg agacagatct ataccaaagc
481 ttgttgcaat tcccaagcct acagtaccac catcagcaga tgaaaaatct aacccaaatt
541 tctttgaaca gagacatgga ggctctcatc agagtagcaa atggactcca gtaggacccg
601 cacccagcac ttctcagtct cagaaacggt cctcaggctt acagagtgga catagtagcc
661 agcggaccag cgcaggtagc agtagtggca ctaacagtag tggtcagagg cacgaccgtg
721 agtcatataa caatagtggg agcagtagcc ggaaaaaagg ccagcatgga tcagaacact
781 ccaaatcacg ttcttccagc cctggaaaac cccaggctgt ttcttcatta aactctagtc
841 attccaggtc tcatgggaat gatcaccata gcaaggaaca tcaacgctcc aaatcacctc
901 gggaccctga tgcaaactgg gattctcctt cccgtgtacc tttttcaagt gggcagcact
961 caactcaatc tttcccaccc tcattgatgt caaagtccaa ttcaatgtta cagaaaccca
1021 ctgcctatgt gcggcccatg gacggacagg agtccatgga accaaagctg tcctctgagc
1081 actacagcag ccaatcccat ggcaacagca tgactgagct gaagcccagc agcaaagcac
1141 atctcaccaa gctgaaaata ccttcccaac cactggatgc atcagcttct ggtgatgtga
1201 gctgtgtgga tgaaatccta aaagagatga cgcattcatg gcctccccct ctaacggcta
1261 ttcatacacc atgcaaaaca gaaccttcca aatttccttt tccaactaag gagtctcagc
1321 agtccaattt tggcactgga gaacaaaggc tcaagtgatc ctcctgcctc agcctactga
1381 gtagctggga ctgtgggcac atgccactat gcctggctga ttttttaaat ttttagtaga
1441 gatagggtct cactatgttg cacaggctgg tctcgaactc ctgagctcaa gtgatccacc
1501 tgcctcgcct cccaaagtgt tgggattact ggtgtgagcc accatgccct cgtatcctaa
1561 tttttatgat ctaatgggta gtagtgggtt gagctgaaaa attgttatgc tttaaaaaat
1621 tcaagaactg taagtcattg gagatcaaaa tacactactt ttattaaaaa aaaaaaaaaa
1681 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1741 aaaaaaaaaa aaa
//