LOCUS BC025347 1430 bp mRNA linear HUM 09-JUN-2008
DEFINITION Homo sapiens ankyrin repeat and LEM domain containing 2, mRNA (cDNA
clone IMAGE:4891015), partial cds.
ACCESSION BC025347
VERSION BC025347.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1430)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1430)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-MAR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 42 Row: i Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1430
/db_xref="H-InvDB:HIT000091052"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4891015"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..1430
/gene="ANKLE2"
/gene_synonym="LEMD7"
/db_xref="GeneID:23141"
/db_xref="HGNC:HGNC:29101"
CDS <1..1326
/gene="ANKLE2"
/gene_synonym="LEMD7"
/codon_start=1
/product="ANKLE2 protein"
/protein_id="AAH25347.1"
/db_xref="GeneID:23141"
/db_xref="HGNC:HGNC:29101"
/translation="DVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFAR
GICDYFPSPSKTSLPLSPVKTAPLFSNDRLKDGLCLSESETVNKERANSYKNPRTQDL
TAKLRKAVEKGEEDTFSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQASIC
QLTLDVLENPDFMRLMYPDDDEAMLQKRIRYVVDLYLNTPDKMGYDTPLHFACKFGNA
DVVNVLSSHHLIVKNSRNKYDKTPEDVICERSKNKSVELKERIREYLKGHYYVPLLRA
EETSSPVIGELWSPDQTAEASHVSRYGGSPRDPVLTLRAFAGPLSPAKAEDFRKLWKT
PPREKAGFLHHVKKSDPERGFERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRL
EEYLTQQEIGKKAQQETGEREASCRDKATTSVEMRFHRIDQAGLELLTS"
BASE COUNT 423 a 327 c 373 g 307 t
ORIGIN
1 gacgtcccag cgagaaatga aaggatctat gtttatgaaa ataaaaagga agcattgcaa
61 gctgtcaaga tgatcaaagg gtcccgattt aaagcttttt ctaccagaga agacgctgag
121 aaatttgcta gaggaatttg tgattatttc ccttctccaa gcaaaacgtc cttaccactg
181 tctcctgtga aaacagctcc actctttagc aatgacaggt tgaaagatgg tttgtgcttg
241 tcggaatcag aaacagtcaa caaagagcga gcgaacagtt acaaaaatcc ccgcacgcag
301 gacctcaccg ccaagcttcg gaaagctgtg gagaagggag aggaggacac cttttctgac
361 cttatctgga gcaacccccg gtatctgata ggctcaggag acaaccccac tatcgtgcag
421 gaagggtgca ggtacaacgt gatgcatgtt gctgccaaag agaaccaggc ttccatctgc
481 cagctgactc tggacgtcct ggagaaccct gacttcatga ggctgatgta ccctgatgac
541 gacgaggcca tgctgcagaa gcgtatccgt tacgtggtgg acctgtacct caacaccccc
601 gacaagatgg gctatgacac accgttgcat tttgcttgta agtttggaaa tgcagatgta
661 gtcaacgtgc tttcgtcaca ccatttgatt gtaaaaaact caaggaataa atatgataaa
721 acacctgaag atgtaatttg tgaaagaagc aaaaataaat ctgtggaact gaaggagcgg
781 atcagagagt atttaaaggg ccactactac gtgcccctcc tgagagcgga agagacttct
841 tctccagtca tcggggagct gtggtcccca gaccagacgg ctgaggcctc tcacgtcagc
901 cgctatggag gcagccccag agacccggta ctgaccctga gagccttcgc agggcccctg
961 agtccagcca aggcagaaga ttttcgcaag ctctggaaaa ctccacctcg agagaaagca
1021 ggcttccttc accacgtcaa gaagtcggac ccggaaagag gctttgagag agtgggaagg
1081 gagctagctc atgagctggg gtatccctgg gttgaatact gggaatttct gggctgtttt
1141 gttgatctgt cttcccagga aggcctgcaa agactagaag aatatctcac acagcaggaa
1201 ataggcaaaa aggctcaaca agaaacagga gaacgggaag cctcctgccg agataaagcc
1261 accacgtctg tggagatgcg gtttcaccgt attgaccagg ctggtctcga actcctgacc
1321 tcgtgatcca ctagagtgct gggaaggtta atgttaaatg ggttgtgtgt cggggagggt
1381 acatgaaaaa taatggcatc gctttgagca gtaaaaaaaa aaaaaaaaaa
//