LOCUS BC025304 2995 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens prune homolog (Drosophila), mRNA (cDNA clone MGC:39274
IMAGE:5458046), complete cds.
ACCESSION BC025304
VERSION BC025304.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2995)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2995)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-MAR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 42 Row: p Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 24308262.
FEATURES Location/Qualifiers
source 1..2995
/db_xref="H-InvDB:HIT000050892"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:39274 IMAGE:5458046"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_47"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2995
/gene="PRUNE"
/gene_synonym="DRES-17"
/gene_synonym="HTCD37"
/db_xref="GeneID:58497"
/db_xref="HGNC:HGNC:13420"
CDS 137..1498
/gene="PRUNE"
/gene_synonym="DRES-17"
/gene_synonym="HTCD37"
/codon_start=1
/product="prune homolog (Drosophila)"
/protein_id="AAH25304.1"
/db_xref="GeneID:58497"
/db_xref="HGNC:HGNC:13420"
/translation="MEDYLQGCRAALQESRPLHVVLGNEACDLDSTVSALALAFYLAK
TTEAEEVFVPVLNIKRSELPLRGDIVFFLQKVHIPESILIFRDEIDLHALYQAGQLTL
ILVDHHILSKSDTALEEAVAEVLDHRPIEPKHCPPCHVSVELVGSCATLVTERILQGA
PEILDRQTAALLHGTIILDCVNMDLKIGKATPKDSKYVEKLEALFPDLPKRNDIFDSL
QKAKFDVSGLTTEQMLRKDQKTIYRQGVKVAISAIYMDLEAFLQRSNLLADLHAFCQA
HSYDVLVAMTIFFNTHNEPVRQLAIFCPHVALQTTICEVLERSHSPPLKLTPASSTHP
NLHAYLQGNTQVSRKKLLPLLQEALSAYFDSMKIPSGQPETADVSREQVDKELDRASN
SLISGLSQDEEDPPLPPTPMNSLVDECPLDQGLPKLSAEAVFEKCSQISLSQSTTASL
SKK"
BASE COUNT 735 a 738 c 674 g 848 t
ORIGIN
1 gcgggttcga gtcccgcctc ctgactctgg cctctagtcc ctgagtcccg ggcgggctgc
61 attcgtcggg gaaacctctc ctcgaccagg ggcacctcta ctcgaccagg ggcgacggcg
121 tactttgggc ttcatcatgg aggactacct gcagggttgt cgagctgctc tgcaggagtc
181 ccgacctcta catgttgtgc tgggaaatga agcctgtgat ttggactcca cagtgtctgc
241 tcttgccctg gctttttacc tagcaaagac aactgaggct gaggaagtct ttgtgccagt
301 tttaaatata aaacgttctg aactacctct gcgaggtgac attgtcttct ttcttcagaa
361 ggttcatatt ccagagagta tcttgatttt tcgggatgag attgacctcc atgcattata
421 ccaggctggc caactcaccc tcatccttgt cgaccatcat atcttatcca aaagtgacac
481 agccctagag gaggcagtag cagaggtgct agaccatcga cccatcgagc cgaaacactg
541 ccctccctgc catgtttcag ttgagctggt ggggtcctgt gctaccctgg tgaccgagag
601 aatcctgcag ggggcaccag agatcttgga caggcaaact gcagcccttc tgcatggaac
661 catcatcctg gactgtgtca acatggacct taaaattgga aaggcaaccc caaaggacag
721 caaatatgtg gagaaactag aggccctttt cccagaccta cccaagagaa atgatatatt
781 tgattcccta caaaaggcaa agtttgatgt atcaggactg accactgagc agatgctgag
841 aaaagaccag aagactatct atagacaagg cgtcaaggtg gccattagtg caatatatat
901 ggatttggag gcctttctgc agaggtctaa cctccttgca gatctccatg ctttctgcca
961 ggctcacagc tatgatgtcc tggttgccat gactatcttt ttcaacactc acaatgagcc
1021 agtgcggcag ttggctattt tctgtcccca tgtggcactc caaacaacga tctgtgaagt
1081 cctggaacgc tcccactctc cacccctgaa gctgacccct gcctcaagta cccaccctaa
1141 cctccatgcc tatcttcaag gcaacaccca ggtctctcga aagaaacttc tgcccctgct
1201 ccaggaagcc ctgtcagcat attttgactc catgaagatc ccttcaggac agcctgagac
1261 agcagatgtg tccagggagc aagtggacaa ggaattggac agggcaagta actccctgat
1321 ttctggactg agtcaagatg aggaggaccc tccgctgccc ccgacgccca tgaacagctt
1381 ggtggatgag tgccctctag atcaggggct gcctaaactc tctgctgagg ccgtcttcga
1441 gaagtgcagt cagatctcac tgtcacagtc taccacagcc tccctgtcca agaagtgact
1501 gttgagaggc gaggaggtag tgggtgaggc tacctgactc acttcaaatg catgttttga
1561 gatgtttgga gattcagcaa ttctgtcttc attgctccag gatctggtat actgttctca
1621 taaaactgag aggagaaaaa aagtgaaaga aagcagctgc tttaagaatg gttttccacc
1681 ttttccccct aatctctacc aatcagacac attttattat ttaaatctgc acctctctct
1741 attttatttg ccaggggcac gatgtgacat atctgcagtc ccagcacagt gggacaaaaa
1801 gaatttagac cccaaaagtg tcctcggcat ggatcttgaa cagaaccagt atctgtcatg
1861 gaactgaaca ttcatcgatg gtctccatgt attcatttat tcacttgttc attcaagtat
1921 ttattgaata cctgcctcaa gctagagaga aaagagagtg cgctttggaa atttattcca
1981 gttttcagcc tacagcagat tatcagctcg gtgacttttc tttctgccac catttaggtg
2041 atggtgtttg attcagagat ggctgaattt ctattcttag cttattgtga ctgtttcaga
2101 tctagtttgg gaacagatta gaggccattg tcttctgtcc tgatcaggtg gcctggctgt
2161 ttctttggat ccctctgtcc cagagccacc cagaaccctg actcttgaga atcaagaaaa
2221 cacccagaaa ggccttaatg acctcatagg cactcttcca aaaagacaac agaactggaa
2281 tgagaggcct gggtctgtct cctgccttag caggcctatc aatttcttgt caatctcttt
2341 ttttccttgc tcacattaaa aggaagcatg gagttctaat gctcccataa actatgtatt
2401 ttggcaagac acttcactac tccaggtctc actttcccca tctgtaaaac agggtttgga
2461 ctaggtgttc cctggtattc tgtgatctgc ctcttgctgc cattctttct ctcctctgct
2521 tctctgtatt tttcttctgt tatccctggg ggtgctcagg ttcacttgat tgtctgtatt
2581 tctgtgtggt tgtagcaagg actcagcctc atgtagcacg aataggggtg tggttcatgg
2641 cgtgttgacc cagcagagca ctccctccca ctaacttgtt ctgcatgtgt agagtctccc
2701 catttttttt aacgcaaccc tttccccttt ttcctacccc acagctctgt tccatgtaag
2761 ttgccaacag tttcactgaa cagtggggta tgtgatggtt ttggcatgac atcttcagta
2821 tgagggggac agtttgactt cactttgagg gtgtgatgtc tgtagctatg tggaaggtaa
2881 aaatagtggt gtgatcatga accaaaggaa tttatgtttt gtaacttggg tactttattt
2941 tgcattttgt tatactatta aataattttt tcctgttaaa aaaaaaaaaa aaaaa
//