LOCUS BC025282 3054 bp mRNA linear HUM 19-JUN-2008
DEFINITION Homo sapiens p21 protein (Cdc42/Rac)-activated kinase 4, mRNA (cDNA
clone MGC:39196 IMAGE:5421365), complete cds.
ACCESSION BC025282
VERSION BC025282.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3054)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3054)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-MAR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 42 Row: n Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 62422555.
FEATURES Location/Qualifiers
source 1..3054
/db_xref="H-InvDB:HIT000039881"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:39196 IMAGE:5421365"
/tissue_type="Pancreas, ductal carcinoma"
/clone_lib="NIH_MGC_110"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3054
/gene="PAK4"
/db_xref="GeneID:10298"
/db_xref="HGNC:HGNC:16059"
/db_xref="MIM:605451"
CDS 428..2203
/gene="PAK4"
/codon_start=1
/product="p21 protein (Cdc42/Rac)-activated kinase 4"
/protein_id="AAH25282.1"
/db_xref="GeneID:10298"
/db_xref="HGNC:HGNC:16059"
/db_xref="MIM:605451"
/translation="MFGKRKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIE
ESARRPKPLVDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRR
DSPPPPARARQENGMPEEPATTARGGPGKAGSRGRFAGHSEAGGGSGDRRRAGPEKRP
KSSREGSGGPQESSRDKRPLSGPDVGTPQPAGLASGAKLAAGRPFNTYPRADTDHPSR
GAQGEPHDVAPNGPSAGGLAIPQSSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPAC
TPAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGI
VCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELW
VVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTH
DGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEM
VDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAEL
LKHPFLAKAGPPASIVPLMRQNRTR"
BASE COUNT 597 a 1010 c 930 g 517 t
ORIGIN
1 gcgggagtgt ccgcggtggt ggcggtgcaa gagagctgag ggaggcgcga gggcgcggag
61 ttccaggtcg agcagttagg ccgcgagcga ctgcggcgcc gagccgatga gtaacccgaa
121 gcccctagag gagtggtcac ctgcctgagg gcacttctgt cccaccagca tcagaccagg
181 tgtgtgcagg ctctctatgg gatgccgcca tcagaatgtc tcttcctcag cccctttgtt
241 ttttaaagtg gctttggtcg accatttgct ctgtgcttgg tactgtgtca agggcccgat
301 tgaagaccac agatcctaaa gataggggag tgtcgcgaca aggctgcctg gggtgccctc
361 ttaacccagg ggatcagtaa ccctgtgctg agtcaatatc caaaggccgc accgagtccc
421 cggcaccatg tttgggaaga ggaagaagcg ggtggagatc tccgcgccgt ccaacttcga
481 gcaccgcgtg cacacgggct tcgaccagca cgagcagaag ttcacggggc tgccccgcca
541 gtggcagagc ctgatcgagg agtcggctcg ccggcccaag cccctcgtcg accccgcctg
601 catcacctcc atccagcccg gggcccccaa gaccatcgtg cggggcagca aaggtgccaa
661 agatggggcc ctcacgctgc tgctggacga gtttgagaac atgtcggtga cacgctccaa
721 ctccctgcgg agagacagcc cgccgccgcc cgcccgtgcc cgccaggaaa atgggatgcc
781 agaggagccg gccaccacgg ccagaggggg cccagggaag gcaggcagcc gaggccggtt
841 cgccggtcac agcgaggcgg gtggcggcag tggtgacagg cgacgggcgg ggccagagaa
901 gaggcccaag tcttccaggg agggctcagg gggtccccag gagtcctccc gggacaaacg
961 ccccctctcc gggcctgatg tcggcacccc ccagcctgct ggtctggcca gtggggcgaa
1021 actggcagct ggccggccct ttaacaccta cccgagggct gacacggacc acccatcccg
1081 gggtgcccag ggggagcctc atgacgtggc ccctaacggg ccatcagcgg ggggcctggc
1141 catcccccag tcctcctcct cctcctcccg gcctcccacc cgagcccgag gtgcccccag
1201 ccctggagtg ctgggacccc acgcctcaga gccccagctg gcccctccag cctgcacccc
1261 cgccgcccct gctgttcctg ggccccctgg cccccgctca ccacagcggg agccacagcg
1321 agtatcccat gagcagttcc gggctgccct gcagctggtg gtggacccag gcgacccccg
1381 ctcctacctg gacaacttca tcaagattgg cgagggctcc acgggcatcg tgtgcatcgc
1441 caccgtgcgc agctcgggca agctggtggc cgtcaagaag atggacctgc gcaagcagca
1501 gaggcgcgag ctgctcttca acgaggtggt aatcatgagg gactaccagc acgagaatgt
1561 ggtggagatg tacaacagct acctggtggg ggacgagctc tgggtggtca tggagttcct
1621 ggaaggaggc gccctcaccg acatcgtcac ccacaccagg atgaacgagg agcagatcgc
1681 ggccgtgtgc cttgcagtgc tgcaggccct gtcggtgctc cacgcccagg gcgtcatcca
1741 ccgggacatc aagagcgact cgatcctgct gacccatgat ggcagggtga agctgtcaga
1801 ctttgggttc tgcgcccagg tgagcaagga agtgccccga aggaagtcgc tggtcggcac
1861 gccctactgg atggccccag agctcatctc ccgccttccc tacgggccag aggtagacat
1921 ctggtcgctg gggataatgg tgattgagat ggtggacgga gagcccccct acttcaacga
1981 gccacccctc aaagccatga agatgattcg ggacaacctg ccaccccgac tgaagaacct
2041 gcacaaggtg tcgccatccc tgaagggctt cctggaccgc ctgctggtgc gagaccctgc
2101 ccagcgggcc acggcagccg agctgctgaa gcacccattc ctggccaagg cagggccgcc
2161 tgccagcatc gtgcccctca tgcgccagaa ccgcaccaga tgaggcccag cgcccttccc
2221 ctcaaccaaa gagccccccg ggtcaccccc gccccactga ggccagtagg gggccaggcc
2281 tcccactcct cccagcccgg gagatgctcc gcgtggcacc accctccttg ctgggggtag
2341 atgagaccct actactgaac tccagttttg atctcgtgac ttttagaaaa acacagggac
2401 tcgtgggagc aagcgaggct cccaggaccc ccaccctctg ggacaggccc tcccccatgt
2461 tcttctgtct ccaggaaggg cagcggccct cccatcactg gaagtctgca gtgggggtcg
2521 ctgggggtgg agagaacact aagaggtgaa catgtatgag tgtgtgcacg cgtgtgagtg
2581 tgcatgtgtg tgtgtgcaaa ggtccagcca ccccgtcctc cagcctgcaa ggggtgtctg
2641 gcgccttgcc tgacacccag ccccctctcc ccctgagcca ttgtgggggt cgatcatgaa
2701 tgtccgaaga gtggcctttt cccgtagccc tgcgccccct ttctgtggct ggatggggag
2761 acaggtcagg gccccccacc ctctccagcc cctgcagcaa atgactactg cacctggaca
2821 gcctcctctt ttctagaagt ctatttatat tgtcatttta taacactcta gcccctgccc
2881 ttattggggg acagatggtc cctgtcctgc ggggtggccc tggcagaacc actgcctgaa
2941 gaaccaggtt cctgcccggt cagcgcagcc ccagcccgcc cacccctgcc tcgagttagt
3001 tttacaatta aaacattgtc ttgttaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//