LOCUS BC024004 4198 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens Rap guanine nucleotide exchange factor (GEF) 4, mRNA
(cDNA clone MGC:26434 IMAGE:4823935), complete cds.
ACCESSION BC024004
VERSION BC024004.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4198)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4198)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (07-FEB-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Sep 16, 2003 this sequence version replaced BC024004.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 33 Row: g Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 5901913.
FEATURES Location/Qualifiers
source 1..4198
/db_xref="H-InvDB:HIT000039718"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:26434 IMAGE:4823935"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..4198
/gene="RAPGEF4"
/gene_synonym="CAMP-GEFII"
/gene_synonym="CGEF2"
/gene_synonym="EPAC2"
/gene_synonym="Nbla00496"
/db_xref="GeneID:11069"
/db_xref="HGNC:HGNC:16626"
/db_xref="MIM:606058"
CDS 27..3062
/gene="RAPGEF4"
/gene_synonym="CAMP-GEFII"
/gene_synonym="CGEF2"
/gene_synonym="EPAC2"
/gene_synonym="Nbla00496"
/codon_start=1
/product="Rap guanine nucleotide exchange factor (GEF) 4"
/protein_id="AAH24004.1"
/db_xref="GeneID:11069"
/db_xref="HGNC:HGNC:16626"
/db_xref="MIM:606058"
/translation="MVAAHAAHSSSSAEWIACLDKRPLERSSEDVDIIFTRLKEVKAF
EKFHPNLLHQICLCGYYENLEKGITLFRQGDIGTNWYAVLAGSLDVKVSETSSHQDAV
TICTLGIGTAFGESILDNTPRHATIVTRESSELLRIEQKDFKALWEKYRQYMAGLLAP
PYGVMETGSNNDRIPDKENTPLIEPHVPLRPANTITKVPSEKILRAGKILRNAILSRA
PHMIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSRTQAVGMWQVLLEDGVLNHVDQ
EHHFQDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRK
PPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGT
SWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDK
EDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGTPEKI
LEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQGTEQEK
MDYALNNKRRVIRLVLQWAAMYGDLLQEDDVSMAFLEEFYVSVSDDARMIAALKEQLP
ELEKIVKQISEDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCMDPTYT
TIRVPVATSVKEVISAVADKLGSGEGLIIVKMSSGGEKVVLKPNDVSVFMTLTINGRL
FACPREQFDSLTPLPEQEGPTVGTVGTFELMSSKDLAYQMTIYDWELFNCVHELELIY
HTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKFIKIAAHCKE
YKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTVA
KLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPD
AAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP"
BASE COUNT 1262 a 880 c 918 g 1138 t
ORIGIN
1 aggggcaccg cgcgccgccg ctcaacatgg tcgctgcgca cgctgcccat tcttcctcct
61 ctgccgagtg gatcgcctgc ctggataaaa gaccactgga gcgatccagc gaagatgtgg
121 atataatctt cactcgactg aaagaagtta aagcttttga gaaatttcac ccaaatctcc
181 ttcatcagat ttgcttatgt ggttattatg agaatctgga aaagggaata acattatttc
241 gccagggtga tattggaaca aactggtatg ctgtcctggc agggtctttg gatgttaaag
301 tatctgagac cagcagtcac caggatgctg tgaccatctg taccctggga attgggacgg
361 cctttggaga gtccattctg gacaacacac cccgccatgc aaccatcgtt accagggaga
421 gcagtgaact gctccgcatc gagcagaagg acttcaaggc actatgggag aaatatcgac
481 agtatatggc aggacttctg gctcctcctt atggtgttat ggaaacgggc tctaacaatg
541 acaggattcc tgacaaggag aacacacctc tcattgaacc tcacgttcct cttcgtcctg
601 ctaacaccat taccaaggtc ccttcagaga agatcctcag agctggaaaa attttacgaa
661 atgccattct ctctcgagca cctcacatga taagagatag aaaataccac ctaaagacat
721 acagacaatg ctgtgtggga actgaactgg tggactggat gatgcagcag acaccatgtg
781 ttcactcccg gactcaagct gttggcatgt ggcaagtcct gttagaagat ggtgttctca
841 accacgtgga ccaggagcac catttccaag acaaatattt attctatcga tttctggatg
901 atgagcacga ggatgcccct ttgcctactg aggaggagaa gaaggagtgt gatgaggagc
961 tccaggacac catgctgctg ctgtcacaga tgggccccga cgcccacatg aggatgatcc
1021 ttcgcaaacc acctggccag aggactgtgg atgacctaga gattatctat gaggagcttc
1081 ttcatattaa agccttatcc catctttcta ccacagtgaa acgagagtta gcaggtgttc
1141 tcatttttga gtctcacgcc aaaggaggga ctgtgttgtt taaccagggg gaagaaggta
1201 cctcctggta cattattcta aaaggatcag tgaatgtagt catttacggc aagggtgtgg
1261 tctgcaccct gcatgaagga gatgacttcg gcaagttagc actagtgaat gatgccccac
1321 gagctgcctc tatcgtctta cgagaagata actgccattt cttaagagta gacaaggagg
1381 atttcaaccg gatcctaagg gacgtggagg cgaatacagt cagacttaaa gaacatgacc
1441 aagatgtctt ggtgctggag aaggtcccag cagggaacag agcttctaat caaggaaact
1501 cacagcctca gcaaaagtat actgtgatgt caggaacacc tgaaaaaatt ttagagcatt
1561 ttctagaaac aatacgcctt gaggcaactt taaatgaagc aacagattct gttttaaatg
1621 actttattat gatgcactgt gtttttatgc caaataccca gctttgcccg gcactggtgg
1681 cccactacca cgcacagcct tcacaaggta cagaacagga gaaaatggat tatgccctca
1741 acaataagag gcgagtcatc cgcctggttc tacagtgggc tgccatgtat ggagacctcc
1801 tgcaagagga tgacgtgtct atggccttcc tggaggagtt ttatgtatct gtatcagatg
1861 atgcccggat gattgctgcc ctcaaggagc aactgccaga gttggagaag attgtcaagc
1921 aaatctcaga agatgcaaag gcaccacaaa agaagcacaa ggttcttttg caacagttca
1981 atacgggcga tgagagagcc cagaagcgcc agcctatccg cggctctgat gaagttctgt
2041 ttaaggtcta ttgcatggac cccacctaca caaccattcg ggtgccagtg gccacttcgg
2101 tgaaggaagt catcagtgca gttgccgaca agctgggctc cggggagggc ctgatcatag
2161 tcaagatgag ttccggagga gaaaaggtgg tgctcaaacc taatgatgtt tcagtattta
2221 tgacgctcac cattaatgga cgcctgtttg cttgcccgcg agagcaattc gattcactga
2281 ctcccttacc agaacaggaa ggcccaactg ttggaacagt gggaactttt gaactgatga
2341 gctccaaaga tttagcatac cagatgacaa tttatgattg ggaactcttc aactgcgtgc
2401 atgagctgga gctaatctat cacacatttg gaaggcataa ttttaaaaag accacagcaa
2461 acttggattt gttcctgagg agatttaatg aaattcagtt ttgggtcgtc actgagatct
2521 gcctttgttc tcagctcagc aagcgtgttc agctattaaa aaaatttatt aagatagcag
2581 cccactgtaa ggagtataaa aatctgaatt ccttttttgc catcgtcatg ggactaagta
2641 acgttgctgt gagccgcttg gcactaacgt gggagaaact gccaagcaag ttcaagaagt
2701 tctatgcgga gtttgaaagt ttaatggacc cttcaaggaa ccacagggcc tacaggctga
2761 cagtagctaa gctggaacct cctctcatcc ccttcatgcc tttgctcatt aaagatatga
2821 catttactca tgaggggaac aagacgttca ttgacaatct agtaaacttt gaaaaaatgc
2881 gcatgattgc aaatacggcc agaacagtga gatactacag gagccaaccc ttcaatcctg
2941 atgcagctca agctaataag aaccatcagg atgtccggag ttatgtacgg caattaaatg
3001 tgattgacaa ccagagaact ttatcacaga tgtcacacag attagagcct cgtcgaccat
3061 agacatttca aatgcccaaa gcaacagttt atctccagtc cacaatcttt caaaaatgcc
3121 atttatgcta ctactgactg tattgccact agagaattct acaaaacaag caaaaacaca
3181 tcctgagaca cctcagggct gcattcagct taccagctac ctagcaagag aaggaattat
3241 ttgacatgcc taaagcttac acactctagc ttaggaatcc ccagtttgtg gctaccctgt
3301 ttcatttcca cttgtgccat cttctttgct gaagtgttga ataaggccca cgtgaagagt
3361 ttggtagaaa tagccttgtc aagagaacta gcaagcccct gacatttttt tctaagagtg
3421 tttatgggat gagatgtgct acaaatggga tagatttgga taaaatttta actcaacatc
3481 ttgatttgga gcctggggtt tcgggaaggt gttgaggaat ctatagaagt ccaaattata
3541 ggttgtattc ttccatcttc attgtcttac ctctgaagga atagaacctg accacattat
3601 tatgaaacat tatggctgtt tatgtaattt agggaggact ggtctgtaat ttctgaatgt
3661 atatagaata atatttatgt ttacaatgta acttttcaaa atttacaaat caggattata
3721 tacataagaa ttccactaag aaatgccaag attcttaaat ttgccagcgt taaagtagaa
3781 aataacattt cagaagagca caatatgcat aaaacatttt tcaaaattga aatattttcc
3841 tgggcattaa aaacctttac tattggctac aaatttattg tacctgatga aaacatattt
3901 tctggactta aatgttatta caaatatctt aattttcagt aattgttttg cactttcaaa
3961 gattgtaaat agttcattca atcaatggta tagagttatt tatttgctac ataatagata
4021 ctgtgccaaa taattacttt ttatttattt tatttagtac gtaattttga agtacatttt
4081 ttcctgtttt cacaattaga ctacatttaa tgtgtaggaa ttgtatgtat gtatatcttc
4141 tgtaaataac atctagtatc ttcactaaat ataattgtcg acaaaaaaaa aaaaaaaa
//