LOCUS BC023632 1307 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens glyceraldehyde-3-phosphate dehydrogenase, mRNA (cDNA
clone MGC:23503 IMAGE:4842418), complete cds.
ACCESSION BC023632
VERSION BC023632.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1307)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1307)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 19, 2003 this sequence version replaced BC023632.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 33 Row: n Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1307
/db_xref="H-InvDB:HIT000050855"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:23503 IMAGE:4842418"
/tissue_type="Eye, normal, pigmented retinal epithelium"
/clone_lib="NIH_MGC_43"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1307
/gene="GAPDH"
/gene_synonym="G3PD"
/db_xref="GeneID:2597"
/db_xref="HGNC:HGNC:4141"
/db_xref="MIM:138400"
CDS 25..1032
/gene="GAPDH"
/gene_synonym="G3PD"
/codon_start=1
/product="glyceraldehyde-3-phosphate dehydrogenase"
/protein_id="AAH23632.1"
/db_xref="GeneID:2597"
/db_xref="HGNC:HGNC:4141"
/db_xref="MIM:138400"
/translation="MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMV
YMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVF
TTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPL
AKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVG
KVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGY
TEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASK
E"
BASE COUNT 364 a 374 c 318 g 251 t
ORIGIN
1 gccgagccac atcgctcaga caccatgggg aaggtgaagg tcggagtcaa cggatttggt
61 cgtattgggc gcctggtcac cagggctgct tttaactctg gtaaagtgga tattgttgcc
121 atcaatgacc ccttcattga cctcaactac atggtttaca tgttccaata tgattccacc
181 catggcaaat tccatggcac cgtcaaggct gagaacggga agcttgtcat caatggaaat
241 cccatcacca tcttccagga gcgagatccc tccaaaatca agtggggcga tgctggcgct
301 gagtacgtcg tggagtccac tggcgtcttc accaccatgg agaaggctgg ggctcatttg
361 caggggggag ccaaaagggt catcatctct gccccctctg ctgatgcccc catgttcgtc
421 atgggtgtga accatgagaa gtatgacaac agcctcaaga tcatcagcaa tgcctcctgc
481 accaccaact gcttagcacc cctggccaag gtcatccatg acaactttgg tatcgtggaa
541 ggactcatga ccacagtcca tgccatcact gccacccaga agactgtgga tggcccctcc
601 gggaaactgt ggcgtgatgg ccgcggggct ctccagaaca tcatccctgc ctctactggc
661 gctgccaagg ctgtgggcaa ggtcatccct gagctgaacg ggaagctcac tggcatggcc
721 ttccgtgtcc ccactgccaa cgtgtcagtg gtggacctga cctgccgtct agaaaaacct
781 gccaaatatg atgacatcaa gaaggtggtg aagcaggcgt cggagggccc cctcaagggc
841 atcctgggct acactgagca ccaggtggtc tcctctgact tcaacagcga cacccactcc
901 tccacctttg acgctggggc tggcattgcc ctcaacgacc actttgtcaa gctcatttcc
961 tggtatgaca acgaatttgg ctacagcaac agggtggtgg acctcatggc ccacatggcc
1021 tccaaggagt aagacccctg gaccaccagc cccagcaaga gcacaagagg aagagagaga
1081 ccctcactgc tggggagtcc ctgccacact cagtccccca ccacactgaa tctcccctcc
1141 tcacagttgc catgtagacc ccttgaagag gggaggggcc tagggagccg caccttgtca
1201 tgtaccatca ataaagtacc ctgtgctcaa ccaaaaaaaa aaaaaaaaac aaaaaaaaaa
1261 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
//