LOCUS BC023566 2770 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens RAS and EF-hand domain containing, mRNA (cDNA clone
MGC:20726 IMAGE:4138119), complete cds.
ACCESSION BC023566
VERSION BC023566.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2770)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2770)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 30 Row: c Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 40255118.
FEATURES Location/Qualifiers
source 1..2770
/db_xref="H-InvDB:HIT000050830"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20726 IMAGE:4138119"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2770
/gene="RASEF"
/gene_synonym="FLJ31614"
/db_xref="GeneID:158158"
/db_xref="HGNC:HGNC:26464"
CDS 262..2484
/gene="RASEF"
/gene_synonym="FLJ31614"
/codon_start=1
/product="RAS and EF-hand domain containing"
/protein_id="AAH23566.1"
/db_xref="GeneID:158158"
/db_xref="HGNC:HGNC:26464"
/translation="MEADGDGEELARLRSVFAACDANRSGRLEREEFRALCTELRVRP
ADAEAVFQRLDADRDGAITFQEFARGFLGSLRGGRRRDWGPLDPAPAVSEAGPETHDS
EEDEGDEDAAAALATSCGPASPGRAWQDFQARLGDEAKFIPREEQVSTLYQNINLVEP
RLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEH
KTRKDEKRKAEEALSDLRRQYETEVGDLQVTIKKLRKLEEQSKRVSQKEDVAALKKQI
YDLSMENQKVKKDLLEAQTNIAFLQSELDALKSDYADQSLNTERDLEIIRAYTEDRNS
LERQIEILQTANRKLHDSNDGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGH
SPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYD
SEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSE
GSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEF
RENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVT
CEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTY
GALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG
"
BASE COUNT 759 a 661 c 761 g 589 t
ORIGIN
1 ctccttggcg gtgcctggag cccgggcgca ccccaccgct cccgggacct gttgggggct
61 ggcccgaacc gtcgtcgaag ggagccgctc ggccaccccc gacgttcctc gccccgcccg
121 acgttccctc aagtggccga accagccgga cgagccaaac tcgccgggcc tcccggcggc
181 agcaggtggc cccgtccttc cagggagggc cctgcgcccc gcggcgctcc ggagccctct
241 cggccgcccc cgccaggcgg gatggaggcg gatggggacg gagaggagct ggcccggctg
301 cgctcagtct tcgccgcctg cgacgcgaac cgctcggggc gcctggagcg cgaggagttc
361 cgggcactgt gcacggagct gcgggtgcgg ccggccgacg ccgaggcagt attccagcgg
421 ctggacgccg accgtgacgg cgccatcacc ttccaggagt tcgcgcgtgg cttcctcggg
481 tccctccgcg gggggcggcg ccgggactgg ggtcctctgg atcccgcgcc cgccgtgtct
541 gaggcggggc cggagacaca cgacagcgag gaggacgaag gcgacgagga cgcggcggcg
601 gcgctggcca cctcgtgcgg cccggcgagt cccggccggg cttggcagga tttccaggcg
661 cgacttgggg acgaagccaa gttcattccc agagaagagc aagttagtac cttgtaccaa
721 aacatcaacc ttgtggagcc aagattaatt cagccatatg aacatgttat aaagaacttc
781 atccgtgaga tcagacttca aagcacagaa atggaaaatt tggccattgc ggtgaagaga
841 gcccaggaca aggcagctat gcagttgagt gagttggaag aggaaatgga tcagaggatt
901 caggctgcag aacataagac acggaaagac gaaaaacgca aagctgagga agccctcagt
961 gacctcagac gtcagtatga aactgaagta ggagatctgc aggtgaccat taaaaagcta
1021 agaaagctcg aagaacaatc aaaacgcgta agtcaaaagg aagatgtggc tgcattgaaa
1081 aaacaaattt atgatttatc aatggaaaac cagaaagtta agaaagacct tttagaagca
1141 cagacaaaca tagcctttct tcagagtgag ttagatgctt tgaaaagtga ttatgctgat
1201 cagagtctga atactgaaag ggatctggaa ataatccgag catacacaga agatcgaaat
1261 agtcttgaga ggcaaattga aatactccaa acagctaacc ggaagctaca tgacagtaat
1321 gatggcctta gaagtgccct tgaaaacagt tatagcaagt tcaacagatc tttgcatata
1381 aataatatct caccagggaa tacaatttct agaagcagtc ccaaattcat tggtcattcc
1441 cctcaacctc taggctatga caggtcatcc cgctcttcct atgtggatga ggactgtgac
1501 tccctggccc tctgtgatcc tctgcagagg acaaattgtg aagttgacag cctgcctgaa
1561 agctgcttcg acagcggctt gtctaccttg agagatccca atgagtatga ctcagaagtg
1621 gaatacaagc accagagggg atttcagagg tcacacgggg tgcaggagag ctttggaggt
1681 gatgcttcag acacagatgt tcctgacata agggatgaag agacatttgg tttagaagat
1741 gtggcttccg tcttagactg gaagccccaa gggtctgtta gtgaaggcag cattgttagt
1801 tcatcaagaa agcccatctc agcactctcg ccccagacag acctggtaga tgacaacgct
1861 aaatctttta gctcacagaa ggcttacaag attgtacttg ctggggacgc tgcagtgggg
1921 aagtctagtt tcctcatgag actttgcaag aatgaatttc gagaaaatat aagcgccacc
1981 ctgggagttg atttccaaat gaaaaccctc attgtggatg gagaacgaac agttctgcag
2041 ctctgggata cagctggtca ggagagattc agaagtattg ccaagtctta cttcagaaag
2101 gcagatggtg ttttgctgct gtatgatgtt acatgtgaga aaagctttct taacatacga
2161 gaatgggtag atatgattga ggatgcagcc catgagactg ttcccattat gctggtagga
2221 aacaaggctg acattcgtga cactgctgct acagagggac aaaaatgtgt cccagggcac
2281 tttggagaga aactggccat gacgtatggg gcattattct gtgaaacaag tgccaaagat
2341 ggttctaaca tagtggaggc tgttctgcac cttgctcgag aagtgaaaaa gagaactgac
2401 aaggatgaca gcagatccat taccaatcta accgggacca attccaaaaa gtcaccacag
2461 atgaagaatt gttgcaatgg ctaaatccca aacatccttg gcctgtgaag tcttcatttc
2521 cagaatactg aatttgtgtg acttatttgg ctcttaacag agtggcacat cctactgaca
2581 ctgtcctatg gagagttaca gtgcaggaaa cctgaaccca gctctcaggt ccctctggaa
2641 ctttggctct tctttgtttt gtctcagtga gtgatttggg ccctctggct aaatagacta
2701 gtcatgtcct tacaggtctt aaaagataac atgtaaatgt ttttaaaatg gtaaaaaaaa
2761 aaaaaaaaaa
//