LOCUS BC023556 1843 bp mRNA linear HUM 06-OCT-2003
DEFINITION Homo sapiens vaccinia related kinase 3, mRNA (cDNA clone MGC:19526
IMAGE:4298578), complete cds.
ACCESSION BC023556
VERSION BC023556.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1843)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1843)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC023556.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 27 Row: n Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7705992.
FEATURES Location/Qualifiers
source 1..1843
/db_xref="H-InvDB:HIT000050826"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:19526 IMAGE:4298578"
/tissue_type="Skin, melanotic melanoma, high MDR."
/clone_lib="NIH_MGC_49"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1843
/gene="VRK3"
/db_xref="GeneID:51231"
CDS 126..1364
/gene="VRK3"
/codon_start=1
/product="VRK3 protein"
/protein_id="AAH23556.1"
/db_xref="GeneID:51231"
/translation="MISFCPDCGKSIQAAFKFCPYCGNSLPVEEHVGSQTFVNPHVSS
FQGSKRGLNSSFETSPKKVKWSSTVTSPRLSLFSDGDSSESEDTLSSSERSKGSGSRP
PTPKSSPQKTRKSPQVTRGSPQKTSCSPQKTRQSPQTLKRSRVTTSLEALPTGTVLTD
KSGRQWKLKSFQTRDNQGILYEAAPTSTLTCDSGPQKQKFSLKLDAKDGRLFNEQNFF
QRAAKPLQVNKWKKLYSTPLLAIPTCMGFGVHQDKYRFLVLPSLGRSLQSALDVSPKH
VLSERSVLQVACRLLDALEFLHENEYVHGNVTAENIFVDPEDQSQVTLAGYGFAFRYC
PSGKHVAYVEGSRSPHEGDLEFISMDLHKGCGPSRRSDLQSLGYCMLKWLYGFLPWTN
CLPNTEDIMKQKQKLPWDSF"
misc_feature 873..1349
/gene="VRK3"
/note="pkinase; Region: Protein kinase domain"
/db_xref="CDD:pfam00069"
BASE COUNT 444 a 529 c 475 g 395 t
ORIGIN
1 ggaggatccg agggtcaggc tgcagaagcc cagaatccca ccccagtccc caagtacaga
61 ggtcgctgtc aagatggagt ttccaaccca gtaaatccaa gggccagacc gtgacctcat
121 aaagcatgat ctccttctgt ccagactgtg gcaaaagtat ccaagcggca ttcaaattct
181 gcccctactg tggaaattct ttgcctgtag aggagcatgt agggtcccag acctttgtca
241 atccacatgt gtcatccttc caaggctcaa agagagggct gaactccagt tttgaaacct
301 ctcctaagaa agtgaaatgg tccagcaccg tcacctctcc ccgattatcc ctcttctcag
361 atggtgacag ttctgagtct gaagatactc tgagttcctc tgagagatcc aaaggctccg
421 ggagcagacc cccaaccccc aaaagcagcc ctcagaagac caggaagagc cctcaggtga
481 ccaggggtag ccctcagaag accagctgta gccctcagaa gaccaggcag agccctcaga
541 cgctgaagcg gagccgagtg accacctcac ttgaagcttt gcccacaggg acagtgctga
601 cagacaagag tgggcgacag tggaagctga agtccttcca gaccagggac aaccagggca
661 ttctctatga agctgcaccc acctccaccc tcacctgtga ctcaggacca cagaagcaaa
721 agttctcact caaactggat gccaaggatg ggcgcttgtt caatgagcag aacttcttcc
781 agcgggccgc caagcctctg caagtcaaca agtggaagaa gctgtactcg accccactgc
841 tggccatccc tacctgcatg ggtttcggtg ttcaccagga caaatacagg ttcttggtgt
901 tacccagcct ggggaggagc cttcagtcgg ccctggatgt cagcccaaag catgtgctgt
961 cagagaggtc tgtgctgcag gtggcctgcc ggctgctgga tgccctggag ttcctccatg
1021 agaatgagta tgttcatgga aatgtgacag ctgaaaatat ctttgtggat ccagaggacc
1081 agagtcaggt gactttggca ggctatggct tcgccttccg ctattgccca agtggcaaac
1141 acgtggccta cgtggaaggc agcaggagcc ctcacgaggg ggaccttgag ttcattagca
1201 tggacctgca caagggatgc gggccctccc gccgcagcga cctccagagc ctgggctact
1261 gcatgctgaa gtggctctac gggtttctgc catggacaaa ttgccttccc aacactgagg
1321 acatcatgaa gcaaaaacag aagttgcctt gggattcatt ttaatgtaag ctggactttg
1381 tcatgccaga aacaaggctc ggtcaccgtc agcagtttgc agttttccac ctcctcccag
1441 ttcctccgtg tggttgaccc agatatctcc gttatgcagc cgcctccggg ggaccacctc
1501 cctccctttg agtcagccac agacagccta cttgacggcc ccgctggccc ccacattcca
1561 ctgaactgtg cggatgccac agtgaccccc tctcaggcac agcatgacct cctgaagtcg
1621 agcctgcttg ctttgaacct accagttaaa atctcctcaa aatgtttgga taccgcccat
1681 tggcccctca cagccacgag ctccctgacc agtgtgcgtg tgtgtgtgtg tgtgtgtctg
1741 tgtgtgtgct tgggacgggt ggggaggtca cctttgggtg tgcggtgtgc ccccaggacc
1801 tgtaagtaat aaaatcttta tttccaaaaa aaaaaaaaaa aaa
//