LOCUS BC022487 2369 bp mRNA linear HUM 28-JUL-2005
DEFINITION Homo sapiens glutamate-cysteine ligase, catalytic subunit, mRNA
(cDNA clone IMAGE:4814728), complete cds.
ACCESSION BC022487
VERSION BC022487.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2369)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2369)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-FEB-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 32 Row: l Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 45359851
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..2369
/db_xref="H-InvDB:HIT000039576"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4814728"
/tissue_type="Brain, hippocampus"
/clone_lib="NIH_MGC_95"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..2369
/gene="GCLC"
/gene_synonym="GCS"
/db_xref="GeneID:2729"
/db_xref="MIM:606857"
CDS 392..1150
/gene="GCLC"
/gene_synonym="GCS"
/codon_start=1
/product="GCLC protein"
/protein_id="AAH22487.1"
/db_xref="GeneID:2729"
/db_xref="MIM:606857"
/translation="MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDV
LKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMI
EGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEV
KPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFI
ETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQV"
BASE COUNT 632 a 550 c 588 g 599 t
ORIGIN
1 aggaagaagc cagtgcacca gaccggcaaa gagaagcggg agccgccgcg gcagcgcggc
61 cgtggggtcc gccgccgccg catcggagcg ggaggaggag cagcggggag ggcgaggccg
121 ccgggccgag agccgtcccg cctgctctcg gtcttctgcc ttcgcctccg cgcggtgcgt
181 cggacccagg gtctgtcacc tgggcgccag gggccgccgc cggggagccg gagcgggcag
241 gaccctccct ccgccgactg cggcccgaga gcgcccccgc ggggtggagc ggcagccgcc
301 ttctgcgggc ggctgagtgt ccgtctcgcg cccggagcgg gcgaccgccg tcagcccgga
361 ggaggaggag gaggaggagg agggggcggc catggggctg ctgtcccagg gctcgccgct
421 gagctgggag gaaaccaagc gccatgccga ccacgtgcgg cggcacggga tcctccagtt
481 cctgcacatc taccacgccg tcaaggaccg gcacaaggac gttctcaagt ggggcgatga
541 ggtggaatac atgttggtat cttttgatca tgaaaataaa aaagtccggt tggtcctgtc
601 tggggagaaa gttcttgaaa ctctgcaaga gaagggggaa aggacaaacc caaaccatcc
661 taccctttgg agaccagagt atgggagtta catgattgaa gggacaccag gacagcccta
721 cggaggaaca atgtccgagt tcaatacagt tgaggccaac atgcgaaaac gccggaagga
781 ggctacttct atattagaag aaaatcaggc tctttgcaca ataacttcat ttcccagatt
841 aggctgtcct gggttcacac tgcccgaggt caaacccaac ccagtggaag gaggagcttc
901 caagtccctc ttctttccag atgaagcaat aaacaagcac cctcgcttca gtaccttaac
961 aagaaatatc cgacatagga gaggagaaaa ggttgtcatc aatgtaccaa tatttaagga
1021 caagaataca ccatctccat ttatagaaac atttactgag gatgatgaag cttcaagggc
1081 ttctaagccg gatcatattt acatggatgc catgggattt ggaatgggca attgctgtct
1141 ccaggtatag tttcaaatat acagagaggc aaagtgttcc atccatttct gttttttaac
1201 ttctttatat atgcatgttt cctgttccaa aaatcacatt ttaatgaggt tgaaatggta
1261 gctggtatgc ctttctgaaa aacatgaagt tatattagta aattcattgg aagctgtcta
1321 tgactaatag ttctacagac tctgttgttc accacaaagg tatatacggt atatatacct
1381 ttataactgt aatttcagtt aacttaaaat gcaatatatt ctgtcattgt ttcccttctc
1441 tttttatatg cctagttttt actgccatct cgtgacaggc tagtgaggtg gtttaaatat
1501 tgttcaaaac tctgaaaatg agctctgatg gaagtattcc aatgattttg tgatccaagg
1561 gatttgtgca aagctgtcta gcggtacaaa aagaataaat atttagcagc ttgtgtttgt
1621 cttgcccctg agtaaagtat acaggagcct gtctctgttt acccatttaa aaattttcct
1681 ggatctgttt aaatgatggt gcccctggca gtgagtgtgt ccaagctcac ttaggaccag
1741 agtggaagag catggaaccc atcaggtggc cacacaaaag cctgcagttg gcttttggct
1801 ctgtcactac tcaggttttt gggcaactcc ctttacttct tttggctcag atttttcatc
1861 agttgaaaga agggctgata ttaagtcccc ttctaggtct gactctaagc taatatttat
1921 tttattgcct taactaacag aagtaaaaga gagaggctgc ttgttattta atgtccaagg
1981 aaattttatc cagttgtgat tacttcagct gattcaaaac agcgtcctta gaggcacagg
2041 gccgtgcaga gcctggaccc atgtaagacc cctctgagcc gccagcctga ctgctctcag
2101 tgcaaagacc cagaacccag ccttccttca cctgaaacag ctgcagcgtg cacacacact
2161 tcttgtgcat atatctctgg tctatttatt ttcaaaacta agtataatcc aaattgaatg
2221 ctggaatcag atttttccat tctgaatgtg tttaaataac atggccaagg ataatttctt
2281 tctgttttct tgacatttat aggtagtata agatatgtga cttccaaata aacataaacc
2341 atcacacttc acgaaaaaaa aaaaaaaaa
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