LOCUS BC022344 3040 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens twinfilin, actin-binding protein, homolog 1
(Drosophila), mRNA (cDNA clone MGC:23788 IMAGE:4249251), complete
cds.
ACCESSION BC022344
VERSION BC022344.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3040)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3040)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-FEB-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 35 Row: d Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: Similarity but not
identity to protein
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..3040
/db_xref="H-InvDB:HIT000039488"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:23788 IMAGE:4249251"
/tissue_type="Prostate"
/clone_lib="NIH_MGC_83"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..3040
/gene="TWF1"
/gene_synonym="A6"
/db_xref="GeneID:5756"
/db_xref="HGNC:HGNC:9620"
/db_xref="MIM:610932"
CDS 392..1150
/gene="TWF1"
/gene_synonym="A6"
/codon_start=1
/product="TWF1 protein"
/protein_id="AAH22344.1"
/db_xref="GeneID:5756"
/db_xref="HGNC:HGNC:9620"
/db_xref="MIM:610932"
/translation="MLYAATRATLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQS
SPAPLTAAEEELRQIKINEVQTDVGVDTKHQTLQGVAFPISREAFQALEKLNNRQLNY
VQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDYLESIVFIYS
MPGYTCSIRERMLYSSCKSRLLEIVERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQ
HAHKQSFAKPKGPAGKRGIRRLIRGPAETEATTD"
BASE COUNT 1036 a 501 c 564 g 939 t
ORIGIN
1 ggctgcactc agcgccggag ccgggagcta gcggccgccg ccatgtccca ccagaccggc
61 atccaaggga agagctggga cagtggaggc gactggggct tcagtacgct gtcgagaacc
121 agcaagtgaa gatgttaaag agatctttgc cagagccaga aatggaaagt acagacttct
181 gaaaatatct attgaaaatg agcaacttgt gattggatca tatagtcagc cttcagattc
241 ctgggataag gattatgatt cctttgtttt acccctgttg gaggacaaac aaccatgcta
301 tatattattc aggttagatt ctcagaatgc ccagggatat gaatggatat tcattgcatg
361 gtctccagat cattctcatg ttcgtcaaaa aatgttgtat gcagcaacaa gagcaactct
421 gaagaaggaa tttggaggtg gccacattaa agatgaagta tttggaacag taaaggaaga
481 tgtatcatta catggatata aaaaatactt gctgtcacaa tcttcccctg ccccactgac
541 tgcagctgag gaagaactac gacagattaa aatcaatgag gtacagactg atgtgggtgt
601 ggacactaag catcaaacac tacaaggagt agcatttccc atttctcgag aagcctttca
661 ggctttggaa aaattgaata atagacagct caactatgtg cagttggaaa tagatataaa
721 aaatgaaatt ataattttgg ccaacacaac aaatacagaa ctgaaagatt tgccaaagag
781 gattcccaag gattcagctc gttaccattt ctttctgtat aaacattccc atgaaggaga
841 ctatttagag tccatagttt ttatttattc aatgcctgga tacacatgca gtataagaga
901 gcggatgctg tattctagct gcaagagccg tctgctagaa attgtagaaa gacaactaca
961 aatggatgta attagaaaga tcgagataga caatggggat gagttgactg cagacttcct
1021 ttatgaagaa gtacatccca agcagcatgc acacaagcaa agttttgcaa aaccaaaagg
1081 tcctgcagga aaaagaggaa ttcgaagact aattaggggc ccagcggaaa ctgaagctac
1141 tactgattaa agtcatcaca ttaaacattg taatactagt tttttaaaag tccagctttt
1201 agtacaggag aactgaaatc attccatgtt gatataaagt agggaaaaaa attgtacttt
1261 ttggaaaata gcacttttca cttctgtgtg tttttaaaat taatgttata gaagactcat
1321 gatttctatt tttgagttaa agctagaaaa gggttcaaca taatgtttaa ttttgtcaca
1381 ctgttttcat agcgttgatt ccacacttca aatacttctt aaaattttat acagttgggc
1441 cagttctaga aagtctgatg tctcaaaggg taaacttact actttcttgt gggacagaaa
1501 gaccttaaaa tattcatatt acttaatgaa tatgttaagg accaggctag agtattttct
1561 aagctggaaa cttagtgtgc cttggaaaag gccgcaagtt gcttactccg agtagctgtg
1621 ctagctctgt cagactgtag gatcatgtct gcaactttta gaaatagtgc tttatattgc
1681 agcagtcttt tatatttgac ttttttttaa tagcattaaa attgcagatc agctcactct
1741 gaaactttaa gggtaccaga tattttctat actgcaggat ttctgatgac attgaaagac
1801 tttaaacagc cttagtaaat tatctttcta atgctctgtg aggccaaaca tttatgttca
1861 gattgaaatt taaattaata tcattcaaaa ggaaacaaaa aatgttgagt tttaaaaatc
1921 aggattgact tttttctcca aaaccataca tttatgggca aattgtgttc tttatcactt
1981 ccgagcaaat actcagattt aaaattactt taaagtcctg gtacttaaca cgctaacgta
2041 gataaacacc ttaataatct cagttaatac tgtatttcaa aacacattta actgttttct
2101 aatgctttgc attatcagtt acaacctaga gagattttga gcctcatatt tctttgatac
2161 ttgaaataga gggagctaga acacttaatg tttaatctgt taaacctgct gcaagagcca
2221 taactttgag gcattttcta aatgaactgt ggggatccag gatttgtaat ttcttgatct
2281 aaactttatg ctgcataaat cacttatcgg aaatgcacat ttcatagtgt gaagcactca
2341 tttctaaacc ttattatcta aggtaatata tgcacctttc agaaatttgt gttcgagtaa
2401 gtaaagcata ttagaataat tgtgggttga cagattttta aaatagaatt tagagtattt
2461 ggggttttgt ttgtttacaa ataatcagac tataatattt aaacatgcaa aataactgac
2521 aataatgttg cacttgttta ctaaagatat aagttgttcc atgggtgtac acgtagacag
2581 acacacatac acccaaatta ttgcattaag aatcctggag cagaccatag ctgaagctgt
2641 tattttcagt caggaagact acctgtcatg aaggtataaa ataatttaga agtgaatgtt
2701 tttctgtacc atctatgtgc aattatactc taaattccac tacactacat taaagtaaat
2761 ggacattcca gaatatagat gtgattatag tcttaaacta attattatta aaccaatgat
2821 tgctgaaaat cagtgatgca tttgttatag agtataactc atcgtttaca gtatgtttta
2881 gttggcagta tcatacctag atggtgaata acatattccc agtaaattta tatagcagtg
2941 aagaattaca tgccttctgg tggacatttt ataagtgcat tttatatcac aataaaaatt
3001 ttttctcttt aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//