LOCUS BC020974 3257 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens sema domain, immunoglobulin domain (Ig), transmembrane
domain (TM) and short cytoplasmic domain, (semaphorin) 4A, mRNA
(cDNA clone MGC:9542 IMAGE:3847802), complete cds.
ACCESSION BC020974
VERSION BC020974.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3257)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3257)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-JAN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 20 Row: i Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361913.
FEATURES Location/Qualifiers
source 1..3257
/db_xref="H-InvDB:HIT000039061"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:9542 IMAGE:3847802"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_65"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3257
/gene="SEMA4A"
/gene_synonym="FLJ12287"
/gene_synonym="RP11-54H19.2"
/gene_synonym="SEMB"
/db_xref="GeneID:64218"
/db_xref="HGNC:HGNC:10729"
/db_xref="MIM:607292"
CDS 198..2483
/gene="SEMA4A"
/gene_synonym="FLJ12287"
/gene_synonym="RP11-54H19.2"
/gene_synonym="SEMB"
/codon_start=1
/product="sema domain, immunoglobulin domain (Ig),
transmembrane domain (TM) and short cytoplasmic domain,
(semaphorin) 4A"
/protein_id="AAH20974.1"
/db_xref="GeneID:64218"
/db_xref="HGNC:HGNC:10729"
/db_xref="MIM:607292"
/translation="MALPALGLDPWSLLGLFLFQLLQLLLPTTTAGGGGQGPMPRVRY
YAGDERRALSFFHQKGLQDFDTLLLSGDGNTLYVGAREAILALDIQDPGVPRLKNMIP
WPASDRKKSECAFKKKSNETQCFNFIRVLVSYNVTHLYTCGTFAFSPACTFIELQDSY
LLPISEDKVMEGKGQSPFDPAHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVL
KTDNFLRWLHHDASFVAAIPSTQVVYFFFEETASEFDFFERLHTSRVARVCKNDVGGE
KLLQKKWTTFLKAQLLCTQPGQLPFNVIRHAVLLPADSPTAPHIYAVFTSQWQVGGTR
SSAVCAFSLLDIERVFKGKYKELNKETSRWTTYRGPETNPRPGSCSVGPSSDKALTFM
KDHFLMDEQVVGTPLLVKSGVEYTRLAVETAQGLDGHSHLVMYLGTTTGSLHKAVVSG
DSSAHLVEEIQLFPDPEPVRNLQLAPTQGAVFVGFSGGVWRVPRANCSVYESCVDCVL
ARDPHCAWDPESRTCCLLSAPNLNSWKQDMERGNPEWACASGPMSRSLRPQSRPQIIK
EVLAVPNSILELPCPHLSALASYYWSHGPAAVPEASSTVYNGSLLLIVQDGVGGLYQC
WATENGFSYPVISYWVDSQDQTLALDPELAGIPREHVKVPLTRVSGGAALAAQQSYWP
HFVTVTVLFALVLSGALIILVASPLRALRARGKVQGCETLRPGEKAPLSREQHLQSPK
ECRTSASDVDADNNCLGTEVA"
BASE COUNT 683 a 1000 c 846 g 728 t
ORIGIN
1 ggcatgatgg gcacctggag ggccgcactc ccgttccagc caggctgagc cttctgtccc
61 ctgcctctgg ggcctgggaa ccccccttct tctttctcct gaatggcacc cccgccctag
121 aatccagaca ccgagtttcc cactgtggct ggttcaaggg tatgtgagag ctccctggtg
181 acagtctgtg gctgagcatg gccctcccag ccctgggcct ggacccctgg agcctcctgg
241 gccttttcct cttccaactg cttcagctgc tgctgccgac gacgaccgcg gggggaggcg
301 ggcaggggcc catgcccagg gtcagatact atgcagggga tgaacgtagg gcacttagct
361 tcttccacca gaagggcctc caggattttg acactctgct cctgagtggt gatggaaata
421 ctctctacgt gggggctcga gaagccattc tggccttgga tatccaggat ccaggggtcc
481 ccaggctaaa gaacatgata ccgtggccag ccagtgacag aaaaaagagt gaatgtgcct
541 ttaagaagaa gagcaatgag acacagtgtt tcaacttcat ccgtgtcctg gtttcttaca
601 atgtcaccca tctctacacc tgcggcacct tcgccttcag ccctgcttgt accttcattg
661 aacttcaaga ttcctacctg ttgcccatct cggaggacaa ggtcatggag ggaaaaggcc
721 aaagcccctt tgaccccgct cacaagcata cggctgtctt ggtggatggg atgctctatt
781 ctggtactat gaacaacttc ctgggcagtg agcccatcct gatgcgcaca ctgggatccc
841 agcctgtcct caagaccgac aacttcctcc gctggctgca tcatgacgcc tcctttgtgg
901 cagccatccc ttcgacccag gtcgtctact tcttcttcga ggagacagcc agcgagtttg
961 acttctttga gaggctccac acatcgcggg tggctagagt ctgcaagaat gacgtgggcg
1021 gcgaaaagct gctgcagaag aagtggacca ccttcctgaa ggcccagctg ctctgcaccc
1081 agccggggca gctgcccttc aacgtcatcc gccacgcggt cctgctcccc gccgattctc
1141 ccacagctcc ccacatctac gcagtcttca cctcccagtg gcaggttggc gggaccagga
1201 gctctgcggt ttgtgccttc tctctcttgg acattgaacg tgtctttaag gggaaataca
1261 aagagttgaa caaagaaact tcacgctgga ctacttatag gggccctgag accaaccccc
1321 ggccaggcag ttgctcagtg ggcccctcct ctgataaggc cctgaccttc atgaaggacc
1381 atttcctgat ggatgagcaa gtggtgggga cgcccctgct ggtgaaatct ggcgtggagt
1441 atacacggct tgcagtggag acagcccagg gccttgatgg gcacagccat cttgtcatgt
1501 acctgggaac caccacaggg tcgctccaca aggctgtggt aagtggggac agcagtgctc
1561 atctggtgga agagattcag ctgttccctg accctgaacc tgttcgcaac ctgcagctgg
1621 cccccaccca gggtgcagtg tttgtaggct tctcaggagg tgtctggagg gtgccccgag
1681 ccaactgtag tgtctatgag agctgtgtgg actgtgtcct tgcccgggac ccccactgtg
1741 cctgggaccc tgagtcccga acctgttgcc tcctgtctgc ccccaacctg aactcctgga
1801 agcaggacat ggagcggggg aacccagagt gggcatgtgc cagtggcccc atgagcagga
1861 gccttcggcc tcagagccgc ccgcaaatca ttaaagaagt cctggctgtc cctaactcca
1921 tcctggagct cccctgcccc cacctgtcag ccttggcctc ttattattgg agtcatggcc
1981 cagcagcagt cccagaagcc tcttccactg tctacaatgg ctccctcttg ctgatagtgc
2041 aggatggagt tgggggtctc taccagtgct gggcaactga gaatggcttt tcataccctg
2101 tgatctccta ctgggtggac agccaggacc agaccctggc cctggatcct gaactggcag
2161 gcatcccccg ggagcatgtg aaggtcccgt tgaccagggt cagtggtggg gccgccctgg
2221 ctgcccagca gtcctactgg ccccactttg tcactgtcac tgtcctcttt gccttagtgc
2281 tttcaggagc cctcatcatc ctcgtggcct ccccattgag agcactccgg gctcggggca
2341 aggttcaggg ctgtgagacc ctgcgccctg gggagaaggc cccgttaagc agagagcaac
2401 acctccagtc tcccaaggaa tgcaggacct ctgccagtga tgtggacgct gacaacaact
2461 gcctaggcac tgaggtagct taaactctag gcacaggccg gggctgcggt gcaggcacct
2521 ggccatgctg gctgggcggc ccaagcacag ccctgactag gatgacagca gcacaaaaga
2581 ccacctttct cccctgagag gagcttctgc tactctgcat cactgatgac actcagcagg
2641 gtgatgcaca gcagtctgcc tcccctatgg gactcccttc taccaagcac atgagctctc
2701 taacagggtg ggggctaccc ccagacctgc tcctacactg atattgaaga acctggagag
2761 gatccttcag ttctggccat tccagggacc ctccagaaac acagtgtttc aagagaccct
2821 aaaaaacctg cctgtcccag gaccctatgg taatgaacac caaacatcta aacaatcata
2881 tgctaacatg ccactcctgg aaactccact ctgaagctgc cgctttggac accaacactc
2941 ccttctccca gggtcatgca gggatctgct ccctcctgct tcccttacca gtcgtgcacc
3001 gctgactccc aggaagtctt tcctgaagtc tgaccacctt tcttcttgct tcagttgggg
3061 cagactctga tcccttctgc cctggcagaa tggcaggggt aatctgagcc ttcttcactc
3121 ctttacccta gctgacccct tcacctctcc ccctcccttt tcctttgttt tgggattcag
3181 aaaactgctt gtcagagact gtttattttt tattaaaaat ataaggctta aaaaaaaaaa
3241 aaaaaaaaaa aaaaaaa
//