LOCUS BC020973 2084 bp mRNA linear SYN 02-SEP-2016
DEFINITION Synthetic construct Homo sapiens RAD23 homolog B (S. cerevisiae),
mRNA (cDNA clone MGC:9444 IMAGE:3906269), complete cds.
ACCESSION BC020973
VERSION BC020973.2
KEYWORDS MGC.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 2084)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2084)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (03-JAN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC020973.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 14 Row: p Column: 2
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 19924138.
FEATURES Location/Qualifiers
source 1..2084
/db_xref="H-InvDB:HIT000039060"
/organism="synthetic construct"
/mol_type="mRNA"
/isolation_source="Homo sapiens; Uterus, leiomyosarcoma"
/db_xref="taxon:32630"
/clone="MGC:9444 IMAGE:3906269"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2084
/gene="RAD23B"
/gene_synonym="HHR23B"
/gene_synonym="HR23B"
/gene_synonym="P58"
/db_xref="GeneID:5887"
/db_xref="MIM:600062"
CDS 51..1280
/gene="RAD23B"
/gene_synonym="HHR23B"
/gene_synonym="HR23B"
/gene_synonym="P58"
/codon_start=1
/transl_table=11
/product="RAD23B protein"
/protein_id="AAH20973.1"
/db_xref="GeneID:5887"
/db_xref="MIM:600062"
/translation="MQVTLKTLQQQTFKIDIDPEETVKALKEKIESEKGKDAFPVAGQ
KLIYAGKILNDDTALKEYKIDEKNFVVVMVTKPKAVSTPAPATTQQSAPASTTAVTSS
TTTTVAQAPTPVPALAPTSTPASITPASATASSEPAPASAAKQEKPAEKPAETPVATS
PTATDSTSGDSSRSNLFEDATSALVTGQSYENMVTEIMSMGYEREQVIAALRASFNNP
DRAVEYLLMGIPGDRESQAVVDPPQAASTGVPQSSAVAAAAATTTATTTTTSSGGHPL
EFLRNQPQFQQMRQIIQQNPSLLPALLQQIGRENPQLLQQISQHQEHFIQMLNEPVQE
AGGQGGGGGGGSGGIAEAGSGHMNYIQVTPQEKEAIERLKALGFPEGLVIQAYFACEK
NENLAANFLLQQNFDED"
misc_feature 51..284
/gene="RAD23B"
/gene_synonym="HHR23B"
/gene_synonym="HR23B"
/gene_synonym="P58"
/note="ubiquitin; Region: Ubiquitin family. This family
contains a number of ubiquitin-like proteins: SUMO (smt3
homologue), Nedd8, Elongin B, Rub1"
/db_xref="CDD:pfam00240"
misc_feature 615..734
/gene="RAD23B"
/gene_synonym="HHR23B"
/gene_synonym="HR23B"
/gene_synonym="P58"
/note="UBA; Region: UBA/TS-N domain. This small domain is
composed of three alpha helices. This family includes the
previously defined UBA and TS-N domains. The UBA-domain
(ubiquitin associated domain) is a novel sequence motif
found in several proteins having connections to ubiquitin
and the ubiquitination pathway. The structure of the UBA
domain consists of a compact three helix bundle. This
domain is found at the N terminus of EF-TS hence the name
TS-N. The structure of EF-TS is known and this domain is
implicated in its interaction with EF-TU. The domain has
been found in non EF-TS proteins such as alpha-NAC and
MJ0280"
/db_xref="CDD:pfam00627"
misc_feature 1146..1256
/gene="RAD23B"
/gene_synonym="HHR23B"
/gene_synonym="HR23B"
/gene_synonym="P58"
/note="UBA; Region: Ubiquitin associated domain"
/db_xref="CDD:smart00165"
BASE COUNT 639 a 427 c 464 g 554 t
ORIGIN
1 cccacgcgtc cgcggacgcg tgggcggacg cgtgggtgcg cggcggcacc atgcaggtca
61 ccctgaagac cctccagcag cagaccttca agatagacat tgaccccgag gagacggtga
121 aagcactgaa agagaagatt gaatctgaaa aggggaaaga tgcctttcca gtagcaggtc
181 aaaaattaat ttatgcaggc aaaatcctca atgatgatac tgctctcaaa gaatataaaa
241 ttgatgagaa aaactttgtg gtggttatgg tgaccaaacc caaagcagtg tccacaccag
301 caccagctac aactcagcag tcagctcctg ccagcactac agcagttact tcctccacca
361 ccacaactgt ggctcaggct ccaacccctg tccctgcctt ggcccccact tccacacctg
421 catccatcac tccagcatca gcgacagcat cttctgaacc tgcacctgct agtgcagcta
481 aacaagagaa gcctgcagaa aagccagcag agacaccagt ggctactagc ccaacagcaa
541 ctgacagtac atcgggtgat tcttctcggt caaacctttt tgaagatgca acgagtgcac
601 ttgtgacggg tcagtcttac gagaatatgg taactgagat catgtcaatg ggctatgaac
661 gagagcaagt aattgcagcc ctgagagcca gtttcaacaa ccctgacaga gcagtggagt
721 atcttttaat gggaatccct ggagatagag aaagtcaggc tgtggttgac ccccctcaag
781 cagctagtac tggggttcct cagtcttcag cagtggctgc agctgcagca actacgacag
841 caacaactac aacaacaagt tctggaggac atccccttga atttttacgg aatcagcctc
901 agtttcaaca gatgagacaa attattcagc agaatccttc cttgcttcca gcgttactac
961 agcagatagg tcgagagaat cctcaattac ttcagcaaat tagccaacac caggagcatt
1021 ttattcagat gttaaatgaa ccagttcaag aagctggtgg tcaaggagga ggaggtggag
1081 gtggcagtgg aggaattgca gaagctggaa gtggtcatat gaactacatt caagtaacac
1141 ctcaggaaaa agaagctata gaaaggttaa aggcattagg atttcctgaa ggacttgtga
1201 tacaagcgta ttttgcttgt gagaagaatg agaatttggc tgccaatttt cttctacagc
1261 agaactttga tgaagattga aagggacttt tttatatctc acacttcaca ccagtgcatt
1321 acactaactt gttcactgga ttgtctggga tgacttgggc tcatatccac aatacttggt
1381 ataaggtagt agattgttgg gggtggggag ggagggatct aggatacagg gcagggataa
1441 atacagtgca tgtctgcttc aattagcaga tgccgcaact ccacacagtg tgtaaaatat
1501 atacaaccaa aaatcagctt ttgcaggtct ttatttcttc tgtaaaacag taggtaactt
1561 ttcctaggtt tcactctttt tagtgtacta gatccagaaa cttagtgtaa tgccctgctt
1621 tatatttctt tgacttaaca ttggtttcag aaagaatctt agctacctag aatttacagt
1681 ctctgtttca tggcaacact ggataatggc tttgtgaaat ttaaaaaatt tttgtagcga
1741 ctgtaaacag aaatgccaaa ttgatggtta attgttgctg cttcaaaaat aagtataaaa
1801 ttaatatgta aggaagccca ttctttcatg ttaaatactt ggggtgggag gggagaaagg
1861 gaaccttttc ttaaaatgaa aataattact gctattttaa aatttcttga tcattgaatg
1921 tgagaccctt ctaacatgat ttgagaagct gtacaagtat aggcagagtt attttcctgt
1981 ttacattttt tttttgtttt ggggaaaaaa ttggtaggtg tctaattact gtttacttca
2041 ttgttatatt gcagtaaaag ttttaaaaca aaaaaaaaaa aaaa
//