LOCUS BC020885 1635 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens chromosome 12 open reading frame 65, mRNA (cDNA clone
MGC:24120 IMAGE:4665283), complete cds.
ACCESSION BC020885
VERSION BC020885.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1635)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1635)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-JAN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 36 Row: j Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 22748626
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..1635
/db_xref="H-InvDB:HIT000039020"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:24120 IMAGE:4665283"
/tissue_type="Testis, embryonal carcinoma"
/clone_lib="NIH_MGC_61"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1635
/gene="C12orf65"
/db_xref="GeneID:91574"
/db_xref="HGNC:HGNC:26784"
CDS 124..483
/gene="C12orf65"
/codon_start=1
/product="C12orf65 protein"
/protein_id="AAH20885.1"
/db_xref="GeneID:91574"
/db_xref="HGNC:HGNC:26784"
/translation="MSTVGLFHFPTPLTRICPAPWGLRLWEKLTLLSPGIAVTPVQMA
GKKDYPALLSLDENELEEQFVKGHGPGGQATNKTSNCVVLKHIPSGIVVKVDHRRPLR
GEAPPKGSTASRDFSQV"
BASE COUNT 445 a 417 c 442 g 331 t
ORIGIN
1 cgtgcgcaga tcagggatcg cgattgcgaa tcctccgctg aggtgatttg gatatcccta
61 gaacgttgag ggcacgagtc gggtcctgag accaggtcct cagccagcag agccacgttc
121 cttatgagca ccgtgggttt atttcatttt cctacaccac tgacccgaat atgcccggcg
181 ccatggggac tccggctttg ggagaagctg acgttgttat ccccaggaat agctgtcact
241 ccggtccaga tggcaggcaa gaaggactac cctgcactgc tttccttgga tgagaatgaa
301 ctcgaagagc agtttgtgaa aggacacggt ccagggggcc aggcaaccaa caaaaccagc
361 aactgcgtgg tgctgaagca catcccctca ggcattgttg taaaggtaga tcacagaagg
421 ccgctgaggg gagaggcccc gcccaagggt tccacagcct ccagagattt ctcccaagtg
481 tgaatgggat cagccgaagt gcattatttt tctggcttgg gccaccctct accagccagg
541 cagtagagag caccgcagat ctcgtcccat ccctgagcca ttcctcccta aggcagaacc
601 tcctacaaca gcctgggaag cctgtttcca ggatcttttg gagccagggt atggctgcca
661 tccttgaatt tggcagacag cactggtgct tggctgctgc cccggaagca acgtcttgac
721 aaagctaagc agtggctgat aaaggcaggc gcccgggcag catggttctt tcaaatccgt
781 tcccactgca ggaaggctgc cgagatcccc agcattgcta tgggatccaa gtccctgagg
841 aggcggcaag cgggcatggg ttgtctgctg gcagtcccag cccttaactg tcatggctgg
901 ttaacattag ggtggccaca tcctccacgc ctctctgtcc cctttggtaa atgtcggtga
961 tacctcctct tctggacaag aggactgagg gcgagatcat ggaggatcat ggaggccagg
1021 cattttccaa cctcagaaga aaaaatgctg tgcacagctg cctataggag tcttcctttt
1081 tacataaaaa aacaagtctc gggccaggca cagtggctca tgcctgtaat cccagcactt
1141 tgggaggccg aggtgggtgg atcacctgag gtcaggagtt cgagaccagt ctggtcaaca
1201 tggcaaaacc ccatctctac tgaaaataca aaaattagcc gggtgtggtg gcgcgtgcct
1261 gtaatcccag ttactcggag gctaaggcag gagaatcgct tgaaccctgg aggcagacat
1321 tgcagtgagc cgagatcatg ctactgcata ccagcctggg caacagacaa gattccctct
1381 taaaaaaaaa aaaaaagaag gttggtcaga ggggctccca gtccatctgc ctcagggatc
1441 acgagagagt cctggaagca gcacagaaga gagatagcca agtgccatca gacaagatca
1501 gttgatcaga acagaaagct agctcggaaa atcctacaag agaaagtaga tgttttctac
1561 aatggtgaaa acagtcctgt tcacaaagaa aaacgagaag cggcgaagaa aaaaaaaaaa
1621 aaaaaaaaaa aaaaa
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