LOCUS BC020862 1227 bp mRNA linear HTC 18-MAR-2009 DEFINITION Homo sapiens transmembrane protein 224, mRNA (cDNA clone IMAGE:4556692), with apparent retained intron. ACCESSION BC020862 VERSION BC020862.1 KEYWORDS HTC. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1227) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. CONSRTM Mammalian Gene Collection Program Team TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (bases 1 to 1227) CONSRTM NIH MGC Project TITLE Direct Submission JOURNAL Submitted (03-JAN-2002) National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: CLONTECH cDNA Library Preparation: CLONTECH Laboratories, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Sequencing Group at the Stanford Human Genome Center, Stanford University School of Medicine, Stanford, CA 94305 Web site: http://www-shgc.stanford.edu Contact: (Dickson, Mark) mcd@paxil.stanford.edu Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers, R. M. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAL Plate: 36 Row: e Column: 14 This clone was selected for full length sequencing because it passed the following selection criteria: Hexamer frequency ORF analysis, GenomeScan gene prediction This clone has the following problem: retained intron. FEATURES Location/Qualifiers source 1..1227 /db_xref="H-InvDB:HIT000090271" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:4556692" /tissue_type="Kidney" /clone_lib="NIH_MGC_75" /lab_host="DH10B" /note="Vector: pDNR-LIB" BASE COUNT 270 a 336 c 352 g 269 t ORIGIN 1 aaaccagctg ctgaaagctg gcggggtggg tctcagactt caggtggcat aataaaataa 61 gaaggaatga ctcatgctgg gacaccgatg gcccgtgaaa acatcatgct aggtgaaggg 121 agccggagat gaaaggccac attcggatgt ctgcctgagc cccaccccga atgccagccc 181 ttcttccctg agctcaccca gccccagctc caggactgag agccccagga agcttggaga 241 ggtgtgagag agacaggtgt ccagcctcag ccccgctcct tcacctgcag agcccggcat 301 gtggggctac ctgtcgctga tgcctgtctt cctagctgtc tgggctatct ctggcgtctg 361 gatcgttttt gccattgcag tgaccaacag gactgtggac ctcagtaaag gctttcccta 421 catcagcatc tgcggatcct tcccccctca gagctgcatc ttcagccagg tgctcaatat 481 gggagctgct ctggccgcgt ggatctgcat tgtccgttac caccagctcc gggactgggg 541 cgtcagaagg tggcctaacc agctgatcct atggacgggt cttctgtgtg ccctgggcac 601 ctccgtggta ggcaatttcc aggtgagacc cagtcggcgc ccagggtctg tcccggccgg 661 cgtctggaac tacaactccc agcatgcccc gtggccatag gcttattgtc tcgggggctg 721 gttcccgccc ggccctcctg ggacttgtat ttttctctgg ccagtggcct tggaccggct 781 ggattccttt gctctctgag tggggcacta cgagcgaggc tgtttgtatg taggacttcg 841 ctgacttcca ggacgttggg ataattttct gccagcccct ctccccagct tatttaatga 901 tgaaattact ggtccaggcg cagtggctca tgcctgtaat cccagcactt tgggaggcgg 961 aggcaggcgg atcgcctgag gtgaggagtt tgagaccagc ctggccaacc aacatggtga 1021 aaccccgtct ctcctaaaat atacaaaaat tagccgggca tggtggcagg cgacttaatc 1081 ccagctactt gggaggcaga ggcaggagaa tcatttgaac ctgggaggtg gaggttgcag 1141 tgagccgaga tcgagccgtt gcactcaaac ctgggggata agagtgagac ttctctcaaa 1201 aaaaaaaaaa aaaaaaaaaa aaaaaaa //