LOCUS BC020544 3422 bp mRNA linear HUM 04-OCT-2003
DEFINITION Homo sapiens NEL-like 2 (chicken), mRNA (cDNA clone MGC:21213
IMAGE:4399991), complete cds.
ACCESSION BC020544
VERSION BC020544.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3422)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3422)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (03-JAN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 27 Row: e Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 5453765.
FEATURES Location/Qualifiers
source 1..3422
/db_xref="H-InvDB:HIT000038812"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:21213 IMAGE:4399991"
/tissue_type="Duodenum, adenocarcinoma"
/clone_lib="NIH_MGC_88"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3422
/gene="NELL2"
/gene_synonym="NRP2"
/db_xref="GeneID:4753"
/db_xref="MIM:602320"
CDS 303..2753
/gene="NELL2"
/gene_synonym="NRP2"
/codon_start=1
/product="NELL2 protein"
/protein_id="AAH20544.1"
/db_xref="GeneID:4753"
/db_xref="MIM:602320"
/translation="MESRVLLRTFCLIFGLGAVWGLGVDPSLQIDVLTELELGESTTG
VRQVPGLHNGTKAFLFQDTPRSIKASTATAEQFFQKLRNKHEFTILVTLKQTHLNSGV
ILSIHHLDHRYLELESSGHRNEVRLHYRSGSHRPHTEVFPYILADDKWHKLSLAISAS
HLILHIDCNKIYERVVEKPSTDLPLGTTFWLGQRNNAHGYFKGIMQDVQLLVMPQGFI
AQCPDLNRTCPTCNDFHGLVQKIMELQDILAKTSAKLSRAEQRMNRLDQCYCERTCTM
KGTTYREFESWIDGCKNCTCLNGTIQCETLICPNPDCPLKSALAYVDGKCCKECKSIC
QFQGRTYFEGERNTVYSSSGVCVLYECKDQTMKLVESSGCPALDCPESHQITLSHSCC
KVCKGYDFCSERHNCMENSICRNLNDRAVCSCRDGFRALREDNAYCEDIDECAEGRHY
CRENTMCVNTPGSFMCICKTGYIRIDDYSCTEHDECITNQHNCDENALCFNTVGGHNC
VCKPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECSDGFVQC
DSRANCINLPGWYHCECRDGYHDNGMFSPSGESCEDIDECGTGRHSCANDTICFNLDG
GYDCRCPHGKNCTGDCIHDGKVKHNGQIWVLENDRCSVCSCQNGFVMCRRMVCDCENP
TVDLFCCPECDPRLSSQCLHQNGETLYNSGDTWVQNCQQCRCLQGEVDCWPLPCPDVE
CEFSILPENECCPRCVTDPCQADTIRNDITKTCLDEMNVVRFTGSSWIKHGTECTLCQ
CKNGHICCSVDPQCLQEL"
misc_feature 387..944
/gene="NELL2"
/gene_synonym="NRP2"
/note="TSPN; Region: Thrombospondin N-terminal -like
domains. Heparin-binding and cell adhesion domain of
thrombospondin"
/db_xref="CDD:smart00210"
misc_feature 1122..1292
/gene="NELL2"
/gene_synonym="NRP2"
/note="vwc; Region: von Willebrand factor type C domain.
The high cutoff was used to prevent overlap with
pfam00094"
/db_xref="CDD:pfam00093"
misc_feature 2220..2378
/gene="NELL2"
/gene_synonym="NRP2"
/note="vwc; Region: von Willebrand factor type C domain.
The high cutoff was used to prevent overlap with
pfam00094"
/db_xref="CDD:pfam00093"
misc_feature 2415..2567
/gene="NELL2"
/gene_synonym="NRP2"
/note="vwc; Region: von Willebrand factor type C domain.
The high cutoff was used to prevent overlap with
pfam00094"
/db_xref="CDD:pfam00093"
BASE COUNT 957 a 719 c 813 g 933 t
ORIGIN
1 ccccgacgga gccgcgccgg ggcgagtccg acccctccct ccgggccccc tccgggccgc
61 gctgccgcct cggccctgcg tgtgggaatg atgtgcgcat tggagggtct aagttcttca
121 cgcgcctggg gaggcctccc ttttctttct taggcaacca aagcgtatta atcctactga
181 tcagtaaatc cgaggcagca gcaggagaga caaacgttat tttcccgctt gattccaaga
241 acctcttcga tttttatttt tatttttaaa gagggagacg atggactgag ctgatccgca
301 ccatggagtc tcgggtctta ctgagaacat tctgtttgat cttcggtctc ggagcagttt
361 gggggcttgg tgtggaccct tccctacaga ttgacgtctt aacagagtta gaacttgggg
421 agtccacgac cggagtgcgt caggtcccgg ggctgcataa tgggacgaaa gcctttctct
481 ttcaagatac tcccagaagc ataaaagcat ccactgctac agctgaacag ttttttcaga
541 agctgagaaa taaacatgaa tttactattt tggtgaccct aaaacagacc cacttaaatt
601 caggagttat tctctcaatt caccacttgg atcacaggta cctggaactg gaaagtagtg
661 gccatcggaa tgaagtcaga ctgcattacc gctcaggcag tcaccgccct cacacagaag
721 tgtttcctta cattttggct gatgacaagt ggcacaagct ctccttagcc atcagtgctt
781 cccatttgat tttacacatt gactgcaata aaatttatga aagggtagta gaaaagccct
841 ccacagactt gcctctaggc acaacatttt ggctaggaca gagaaataat gcgcatggat
901 attttaaggg tataatgcaa gatgtccaat tacttgtcat gccccaggga tttattgctc
961 agtgcccaga tcttaatcgc acctgtccaa cttgcaatga cttccatgga cttgtgcaga
1021 aaatcatgga gctacaggat attttagcca aaacatcagc caagctgtct cgagctgaac
1081 agcgaatgaa tagattggat cagtgctatt gtgaaaggac ttgcaccatg aagggaacca
1141 cctaccgaga atttgagtcc tggatagacg gctgtaagaa ctgcacatgc ctgaatggaa
1201 ccatccagtg tgaaactcta atctgcccaa atcctgactg cccacttaag tcggctcttg
1261 cgtatgtgga tggcaaatgc tgtaaggaat gcaaatcgat atgccaattt caaggacgaa
1321 cctactttga aggagaaaga aatacagtct attcctcttc tggagtatgt gttctctatg
1381 agtgcaagga ccagaccatg aaacttgttg agagttcagg ctgtccagct ttggattgtc
1441 cagagtctca tcagataacc ttgtctcaca gctgttgcaa agtttgtaaa ggttatgact
1501 tttgttctga aaggcataac tgcatggaga attccatctg cagaaatctg aatgacaggg
1561 ctgtttgtag ctgtcgagat ggttttaggg ctcttcgaga ggataatgcc tactgtgaag
1621 acatcgatga gtgtgctgaa gggcgccatt actgtcgtga aaatacaatg tgtgtcaaca
1681 ccccgggttc ttttatgtgc atctgcaaaa ctggatacat cagaattgat gattattcat
1741 gtacagaaca tgatgagtgt atcacaaatc agcacaactg tgatgaaaat gctttatgct
1801 tcaacactgt tggaggacac aactgtgttt gcaagccggg ctatacaggg aatggaacga
1861 catgcaaagc attttgcaaa gatggctgta ggaatggagg agcctgtatt gccgctaatg
1921 tgtgtgcctg cccacaaggc ttcactggac ccagctgtga aacggacatt gatgaatgct
1981 ctgatggttt tgttcaatgt gacagtcgtg ctaattgcat taacctgcct ggatggtacc
2041 actgtgagtg cagagatggc taccatgaca atgggatgtt ttcaccaagt ggagaatcgt
2101 gtgaagatat tgatgagtgt gggaccggga ggcacagctg tgccaatgat accatttgct
2161 tcaatttgga tggcggatat gattgtcgat gtcctcatgg aaagaattgc acaggggact
2221 gcatccatga tggaaaagtt aagcacaatg gtcagatttg ggtgttggaa aatgacaggt
2281 gctctgtgtg ctcatgtcag aatggattcg ttatgtgtcg acggatggtc tgtgactgtg
2341 agaatcccac agttgatctt ttttgctgcc ctgaatgtga cccaaggctt agtagtcagt
2401 gcctccatca aaatggggaa actttgtata acagtggtga cacctgggtc cagaattgtc
2461 aacagtgccg ctgcttgcaa ggggaagttg attgttggcc cctgccttgc ccagatgtgg
2521 agtgtgaatt cagcattctc ccagagaatg agtgctgccc gcgctgtgtc acagaccctt
2581 gccaggctga caccatccgc aatgacatca ccaagacttg cctggacgaa atgaatgtgg
2641 ttcgcttcac cgggtcctct tggatcaaac atggcactga gtgtactctc tgccagtgca
2701 agaatggcca catctgttgc tcagtggatc cacagtgcct tcaggaactg tgaagttaac
2761 tgtctcatgg gagatttctg ttaaaagaat gttctttcat taaaagacca aaaagaagtt
2821 aaaacttaaa ttgggtgatt tgtgggcagc taaatgcagc tttgttaata gctgagtgaa
2881 ctttcaatta tgaaatttgt ggagcttgac aaaatcacaa aaggaaaatt actggggcaa
2941 aattagacct caagtctgcc tctactgtgt ctcacatcac catgtagaag aatgggcgta
3001 cagtatatac cgtgacatcc tgaaccctgg atagaaagcc tgagcccatt ggatctgtga
3061 aagcctctag cttcactggt gcagaaaatt ttcctctaga tcagaatctt caagaatcag
3121 ttaggttcct cactgcaaga aataaaatgt caggcagtga atgaattata ttttcagaag
3181 taaagcaaag aagctataac atgttgtgta cagtacactc tgaaaagaaa tctgaaacaa
3241 gttattgtaa tgataaaaat aatgcacagg catggttact taatattttc taacaggaaa
3301 agtcatccct atttccttgt tttactgcac ttaatattat ttggttgaat ttgttcagta
3361 taagctcgtt cttgtgcaaa attaaataaa tatttctctt accttataaa aaaaaaaaaa
3421 aa
//