LOCUS BC020393 1747 bp mRNA linear SYN 02-SEP-2016
DEFINITION Synthetic construct Homo sapiens structural maintenance of
chromosomes 5, mRNA (cDNA clone IMAGE:4156864).
ACCESSION BC020393
VERSION BC020393.1
KEYWORDS HTC.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 1747)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1747)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-JAN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 12 Row: e Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis
This clone has the following problem: no polyA-tail.
FEATURES Location/Qualifiers
source 1..1747
/db_xref="H-InvDB:HIT000090105"
/organism="synthetic construct"
/mol_type="mRNA"
/isolation_source="Homo sapiens; Brain, anaplastic
oligodendroglioma with 1p/19q loss"
/db_xref="taxon:32630"
/clone="IMAGE:4156864"
/clone_lib="NCI_CGAP_Brn67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
BASE COUNT 633 a 232 c 279 g 603 t
ORIGIN
1 tgtttttaaa attttggcca gagtctacaa acaatgattg taaaaaagtt agtccaatac
61 aagataatct gccaataatg taagcataac tatctccctt ctgtatcatt ttgaagtaat
121 tcccagacat tctgtttcac ctgaaaatat tttggtatgt atctctaaaa gaagtggact
181 ctttttataa aaacacataa ctacagtatt accacagtaa aaaaatttat actaagtaac
241 atctaatatg ccgtgttcga gtttcttatc catcccacga atgccagtag gagtatggta
301 actataaatc atttcagtag agaatatatg gtaattaaaa taatattttc ttttccctat
361 attcaggttc gtgacaataa aaaattaaga gtaaatgctg ttattgctcc caagagttca
421 tatgcagaca aagcaccttc aagatctttg aatgaactta agtaagtctt gaaaatacta
481 agatttattt aaatattgaa ttattcaaca tttattgtta cataaataag ctattacaag
541 ctgtattatt tccttttaag atctcttaag tcgaatataa ggaaaaattt aggcacagga
601 ccatgatata gttagcacac agtgagtgtg atgcgatgtg atgtttcaag tctgcagaat
661 aatattgaac tatttaataa aagatgctaa agtagtgggt tagttattgg gaaaatgaaa
721 tgataaatta tcccttcata atgcagaaac aatttccaaa aggactaaat gtaaaaaatg
781 ttaattataa aataattata agtaaatatc ttaatttgga atataagtaa gcattctaaa
841 cataatagaa gaaatttaaa aagagtagtt tgtaatcata aaaagcttaa gcttttctgg
901 taagtgaata agtcatatgt gattttaaaa tataaaaaaa agaaaaaaga cttgtcctgt
961 gtcattgttg aaataatgta tgttttactt attgtttatt ggtggcttat caactcccag
1021 atttttttaa tgatatttta agaagaaaaa tctaatcaat acttttcctt tcagacaata
1081 cggatttttc tcttatttga gagaattatt tgatgcacct gatcctgtaa tgagttacct
1141 ttgctgtcag tatcatattc atgaagttcc tgtaggaact gaaaagacca gagaaagaat
1201 tgaacgggta ggaaagtagt gaatcatgta ctgaatcatg taccaaaaat gtagttttgt
1261 taaaacttct tagctaaagg caagcaaaac gtgtcagaat ttaagtaagt ctgttgtttt
1321 taaccatgca gaaattatat ttgtattaat tattttccat atgacatgaa acattgactg
1381 ttaactaata cattgtgaga ggagtgatgt ggaattaact agaataaata cttaatacat
1441 ttagggtaat tccataaagt taaaacctga aaagagatac ctagtacact aacctgattc
1501 tgccttgctt ggtaaatcta tgttcaaaca ttgtattaat agtttagtct tttaaattat
1561 tatatttgag attattttta agacaaatta tactttagaa tggtaggctg gggtataaaa
1621 ctgtttttgg tcagccttcc ttttattaaa acttgtactt aagattatct cagatatgaa
1681 tgaccacttc tcataggatt tgtttggcta gtacatacag gactagttct agatcgcgag
1741 cggccgc
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