LOCUS BC019088 2347 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens ER degradation enhancer, mannosidase alpha-like 1,
mRNA (cDNA clone MGC:29498 IMAGE:4863779), complete cds.
ACCESSION BC019088
VERSION BC019088.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2347)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2347)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (07-DEC-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 39 Row: h Column: 20
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7662001.
FEATURES Location/Qualifiers
source 1..2347
/db_xref="H-InvDB:HIT000038541"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:29498 IMAGE:4863779"
/tissue_type="Skin, melanotic melanoma, high MDR."
/clone_lib="NIH_MGC_49"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2347
/gene="EDEM1"
/gene_synonym="EDEM"
/gene_synonym="KIAA0212"
/db_xref="GeneID:9695"
/db_xref="HGNC:HGNC:18967"
/db_xref="MIM:607673"
CDS 140..2113
/gene="EDEM1"
/gene_synonym="EDEM"
/gene_synonym="KIAA0212"
/codon_start=1
/product="ER degradation enhancer, mannosidase alpha-like
1"
/protein_id="AAH19088.1"
/db_xref="GeneID:9695"
/db_xref="HGNC:HGNC:18967"
/db_xref="MIM:607673"
/translation="MQWRALVLGLVLLRLGLHGVLWLVFGLGPSMGFYQRFPLSFGFQ
RLRSPDGPASPTSGPVGRPGGVSGPSWLQPPGTGAAQSPRKAPRRPGPGMCGPANWGY
VLGGRGRGPDEYEKRYSGAFPPQLRAQMRDLARGMFVFGYDNYMAHAFPQDELNPIHC
RGRGPDRGDPSNLNINDVLGNYSLTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKD
STVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFE
NTKTGIPYPRVNLKTGVPPDTNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVK
ALWNLRSNDTGLLGNVVNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMF
NAAYQSIQNYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIG
DVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNP
FYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDN
PVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVIN
SSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI"
BASE COUNT 570 a 615 c 646 g 516 t
ORIGIN
1 gcacgaggct agttcctggt gcgtcacggg ggagttcctt aaaggggaag cgagccgggc
61 tacggggcga gcgcggggtg cggtggtcgg cggggaggcc cccgcgcttt aaaataatgc
121 ccgcggcgcc cgcgcgacca tgcaatggcg agcgctcgtc ctggggctgg tgctcctccg
181 gcttggcctc catggagtat tgtggctcgt cttcgggctg gggcccagca tgggcttcta
241 ccagcgcttt ccgctcagct tcggcttcca gcgtctgagg agccccgacg gccccgcgtc
301 gcccacctcg gggcccgtgg gccggcctgg gggggtatcc gggccgtcgt ggctgcagcc
361 gccggggacc ggggcagcgc agagcccgcg caaggctccg cggcgtcctg ggccggggat
421 gtgcggccca gccaactggg gctacgtgct gggcggccgg ggccgcggcc cggacgagta
481 cgagaagcgc tacagcggcg ccttccctcc gcagctgcgt gcccagatgc gcgacctggc
541 acggggcatg ttcgtctttg gctacgacaa ctacatggct cacgccttcc cccaggacga
601 gctcaacccc atccactgcc gcggccgtgg gcccgaccgc ggggaccctt caaatctgaa
661 catcaatgat gtactaggga actactcatt gactcttgtt gatgcattgg atacacttgc
721 aataatggga aattcatccg agttccagaa agcagtcaag ttagtgatca acacagtttc
781 atttgacaaa gattccaccg tccaagtctt tgaggccacg ataagggtcc tgggaagcct
841 cctttctgct cacagaataa taactgactc caagcagccc tttggtgaca tgacaattaa
901 ggactatgat aatgagttgt tatacatggc ccatgacctg gcggtgcggc tcctccctgc
961 ttttgaaaac accaagacag ggattccata tcctcgggtg aatctaaaga caggagttcc
1021 tcctgacacc aataatgaga catgcacagc gggagccggt tccctcctgg tggaatttgg
1081 gattctgagt cgactcctgg gggactccac atttgagtgg gtggccagac gagcagtgaa
1141 agccctttgg aacctccgga gcaatgatac aggattacta ggcaatgtcg tgaacattca
1201 gacgggccac tgggttggaa agcagagtgg cctgggtgcc gggctggact ccttctatga
1261 atacctcttg aaatcttaca ttctctttgg agaaaaagaa gacctagaaa tgtttaatgc
1321 tgcatatcag agtattcaga actacttaag aagagggcgg gaagcctgca atgaaggaga
1381 aggagaccct ccactctatg tcaacgtgaa catgttcagt gggcagctga tgaacacctg
1441 gattgactct ctgcaggcct ttttccctgg actgcaggtg ctgataggag atgtggaaga
1501 tgccatctgc cttcatgcct tctactatgc catatggaaa cgatatggtg ccctccctga
1561 gagatataac tggcagctgc aggcccctga cgttctcttc tacccactga gaccagagtt
1621 agtggaatcc acatatctcc tctaccaggc aaccaagaat cccttctacc tccatgtagg
1681 aatggatatt ctgcagagtc tggaaaagta cacaaaagtc aagtgtgggt acgccacgct
1741 gcatcacgtc attgacaagt ccacagaaga ccggatggag agcttctttc tcagtgagac
1801 ctgtaaatat ttgtatctgc tgtttgatga agacaatcca gtacacaagt ctggaaccag
1861 atacatgttc acaacagagg gacacattgt atctgtggat gagcatcttc gggaattgcc
1921 atggaaggaa ttcttctctg aagagggagg gcaggaccaa gggggaaagt ctgtgcacag
1981 gccgaaacct catgagttaa aagtcatcaa ctccagctcc aactgcaatc gtgtacctga
2041 tgagaggagg tactccctgc ccttaaagag catctacatg cgacagattg accagatggt
2101 tggtttgatt tgatctgctc tctgtgaggc ctcatcttga accagacctt aacgaccaaa
2161 cccagaccat gccaaagtcc agtctgaaat gaaaggggac agaagtcttg ctgtccatgg
2221 tggtgtagga atttctgtgc aacacctcac cacgtctggt taatccttgc acacttcagt
2281 gtttctctcc tgttcaataa aatgccctgt taaggatata aaaaaaaaaa aaaaaaaaaa
2341 aaaaaaa
//