LOCUS BC018119 3216 bp mRNA linear HUM 11-AUG-2006
DEFINITION Homo sapiens v-raf-1 murine leukemia viral oncogene homolog 1, mRNA
(cDNA clone MGC:9026 IMAGE:3904404), complete cds.
ACCESSION BC018119
VERSION BC018119.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3216)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3216)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-DEC-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC018119.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 22 Row: h Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 52486392.
FEATURES Location/Qualifiers
source 1..3216
/db_xref="H-InvDB:HIT000038277"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:9026 IMAGE:3904404"
/tissue_type="Pancreas, epithelioid carcinoma"
/clone_lib="NIH_MGC_70"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3216
/gene="RAF1"
/gene_synonym="c-Raf"
/gene_synonym="CRAF"
/gene_synonym="Raf-1"
/db_xref="GeneID:5894"
/db_xref="HGNC:HGNC:9829"
/db_xref="MIM:164760"
CDS 330..2276
/gene="RAF1"
/gene_synonym="c-Raf"
/gene_synonym="CRAF"
/gene_synonym="Raf-1"
/codon_start=1
/product="v-raf-1 murine leukemia viral oncogene homolog
1"
/protein_id="AAH18119.1"
/db_xref="GeneID:5894"
/db_xref="HGNC:HGNC:9829"
/db_xref="MIM:164760"
/translation="MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASD
DGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLL
HEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLL
NGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMR
ESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRM
IEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRG
QRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRN
EVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIAR
QTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGS
VLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYA
SPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPS
LHRAAHTEDINACTLTTSPRLPVF"
BASE COUNT 827 a 804 c 810 g 775 t
ORIGIN
1 tcgcgggcgc ttgggccgcc atcttagatg gcgggagtaa gaggaaaacg attgtgaggc
61 gggaacggct ttctgctgcc ttttttgggc cccgaaaagg gtcagctggc cgggctttgg
121 ggcgcgtgcc ctgaggcgcg gagcgcgttt gctacgatgc gggggctgct cggggctccg
181 tcccctgggc tggggacgcg ccgaatgtga ccgcctcccg ctccctcacc cgccgcgggg
241 aggaggagcg ggcgagaagc tgccgccgaa cgacaggacg ttggggcggc ctggctccct
301 caggtttaag aattgtttaa gctgcatcaa tggagcacat acagggagct tggaagacga
361 tcagcaatgg ttttggattc aaagatgccg tgtttgatgg ctccagctgc atctctccta
421 caatagttca gcagtttggc tatcagcgcc gggcatcaga tgatggcaaa ctcacagatc
481 cttctaagac aagcaacact atccgtgttt tcttgccgaa caagcaaaga acagtggtca
541 atgtgcgaaa tggaatgagc ttgcatgact gccttatgaa agcactcaag gtgaggggcc
601 tgcaaccaga gtgctgtgca gtgttcagac ttctccacga acacaaaggt aaaaaagcac
661 gcttagattg gaatactgat gctgcgtctt tgattggaga agaacttcaa gtagatttcc
721 tggatcatgt tcccctcaca acacacaact ttgctcggaa gacgttcctg aagcttgcct
781 tctgtgacat ctgtcagaaa ttcctgctca atggatttcg atgtcagact tgtggctaca
841 aatttcatga gcactgtagc accaaagtac ctactatgtg tgtggactgg agtaacatca
901 gacaactctt attgtttcca aattccacta ttggtgatag tggagtccca gcactacctt
961 ctttgactat gcgtcgtatg cgagagtctg tttccaggat gcctgttagt tctcagcaca
1021 gatattctac acctcacgcc ttcaccttta acacctccag tccctcatct gaaggttccc
1081 tctcccagag gcagaggtcg acatccacac ctaatgtcca catggtcagc accaccctgc
1141 ctgtggacag caggatgatt gaggatgcaa ttcgaagtca cagcgaatca gcctcacctt
1201 cagccctgtc cagtagcccc aacaatctga gcccaacagg ctggtcacag ccgaaaaccc
1261 ccgtgccagc acaaagagag cgggcaccag tatctgggac ccaggagaaa aacaaaatta
1321 ggcctcgtgg acagagagat tcaagctatt attgggaaat agaagccagt gaagtgatgc
1381 tgtccactcg gattgggtca ggctcttttg gaactgttta taagggtaaa tggcacggag
1441 atgttgcagt aaagatccta aaggttgtcg acccaacccc agagcaattc caggccttca
1501 ggaatgaggt ggctgttctg cgcaaaacac ggcatgtgaa cattctgctt ttcatggggt
1561 acatgacaaa ggacaacctg gcaattgtga cccagtggtg cgagggcagc agcctctaca
1621 aacacctgca tgtccaggag accaagtttc agatgttcca gctaattgac attgcccggc
1681 agacggctca gggaatggac tatttgcatg caaagaacat catccataga gacatgaaat
1741 ccaacaatat atttctccat gaaggcttaa cagtgaaaat tggagatttt ggtttggcaa
1801 cagtaaagtc acgctggagt ggttctcagc aggttgaaca acctactggc tctgtcctct
1861 ggatggcccc agaggtgatc cgaatgcagg ataacaaccc attcagtttc cagtcggatg
1921 tctactccta tggcatcgta ttgtatgaac tgatgacggg ggagcttcct tattctcaca
1981 tcaacaaccg agatcagatc atcttcatgg tgggccgagg atatgcctcc ccagatctta
2041 gtaagctata taagaactgc cccaaagcaa tgaagaggct ggtagctgac tgtgtgaaga
2101 aagtaaagga agagaggcct ctttttcccc agatcctgtc ttccattgag ctgctccaac
2161 actctctacc gaagatcaac cggagcgctt ccgagccatc cttgcatcgg gcagcccaca
2221 ctgaggatat caatgcttgc acgctgacca cgtccccgag gctgcctgtc ttctagttga
2281 ctttgcacct gtcttcaggc tgccagggga ggaggagaag ccagcaggca ccacttttct
2341 gctccctttc tccagaggca gaacacatgt tttcagagaa gctgctgcta aggaccttct
2401 agactgctca cagggcctta acttcatgtt gccttctttt ctatcccttt gggccctggg
2461 agaaggaagc catttgcagt gctggtgtgt cctgctccct ccccacattc cccatgctca
2521 aggcccagcc ttctgtagat gcgcaagtgg atgttgatgg tagtacaaaa agcaggggcc
2581 cagccccagc tgttggctac atgagtattt agaggaagta aggtagcagg cagtccagcc
2641 ctgatgtgga gacacatggg attttggaaa tcagcttctg gaggaatgca tgtcacaggc
2701 gggactttct tcagagagtg gtgcagcgcc agacattttg cacataaggc accaaacagc
2761 ccaggactgc cgagactctg gccgcccgaa ggagcctgct ttggtactat ggaacttttc
2821 ttaggggaca cgtcctcctt tcacagcttc taaggtgtcc agtgcattgg gatggttttc
2881 caggcaaggc actcggccaa tccgcatctc agccctctca gggagcagtc ttccatcatg
2941 ctgaattttg tcttccagga gctgccccta tggggcgggg ccgcagggcc agccttgttt
3001 ctctaacaaa caaacaaaca aacagccttg tttctctagt cacatcatgt gtatacaagg
3061 aagccaggaa tacaggtttt cttgatgatt tgggttttaa ttttgttttt attgcacctg
3121 acaaaataca gttatctgat ggtccctcaa ttatgttatt ttaataaaat aaattaaatt
3181 taaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa
//