LOCUS BC017707 2218 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens midline 2, mRNA (cDNA clone MGC:21197 IMAGE:4446324),
complete cds.
ACCESSION BC017707
VERSION BC017707.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2218)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2218)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-DEC-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 27 Row: o Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 16445407.
FEATURES Location/Qualifiers
source 1..2218
/db_xref="H-InvDB:HIT000038091"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:21197 IMAGE:4446324"
/tissue_type="Kidney, hypernephroma"
/clone_lib="NIH_MGC_89"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2218
/gene="MID2"
/gene_synonym="FXY2"
/gene_synonym="MID1"
/gene_synonym="RNF60"
/gene_synonym="TRIM1"
/db_xref="GeneID:11043"
/db_xref="HGNC:HGNC:7096"
/db_xref="MIM:300204"
CDS 28..2085
/gene="MID2"
/gene_synonym="FXY2"
/gene_synonym="MID1"
/gene_synonym="RNF60"
/gene_synonym="TRIM1"
/codon_start=1
/product="midline 2"
/protein_id="AAH17707.1"
/db_xref="GeneID:11043"
/db_xref="HGNC:HGNC:7096"
/db_xref="MIM:300204"
/translation="METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSS
GESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRR
ERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHR
LVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEK
LKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQR
KQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKN
IAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELC
TASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTV
HGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEK
DESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNE
WIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSL
HLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMK
TCH"
BASE COUNT 644 a 548 c 485 g 541 t
ORIGIN
1 gcctcaggag gactattttc actaaagatg gaaacactgg agtctgaatt gacctgtcca
61 atctgcctag agttgtttga agaccccctt ctgctccctt gtgctcacag cctctgcttc
121 agctgtgccc atcgcatttt ggtatcaagc tgcagctctg gtgaatccat tgaacccatt
181 actgctttcc agtgtcctac ctgcaggtat gttatctcgc tgaaccaccg gggcctggat
241 ggcctcaaga ggaatgtgac tctgcagaac attattgatc gcttccagaa ggcttcagtc
301 agtgggccca attcccctag tgagagccgc cgggaaagga cttacaggcc caccactgcc
361 atgtctagcg agcgaattgc ttgccaattc tgtgagcagg acccgccaag ggatgcagta
421 aaaacatgca tcacctgtga ggtctcctac tgtgaccgtt gcctgcgggc cacgcacccc
481 aacaagaaac ctttcaccag ccaccgcctg gtggaaccag tgccagacac acatcttcga
541 gggatcacct gcctggacca tgagaatgag aaagtgaaca tgtactgtgt atctgatgac
601 caattgatct gtgccttatg caaactggtg ggtcgtcacc gagaccatca ggtcgcatcc
661 ctgaatgatc gatttgagaa actcaagcaa actctggaga tgaacctcac caacctggtt
721 aagcgcaaca gcgaactaga aaatcaaatg gccaaactaa tacagatctg ccagcaggtt
781 gaggtgaata ctgctatgca tgaggcaaaa cttatggaag aatgtgacga gttggtagag
841 atcatccagc agaggaagca aatgatcgct gtcaaaatca aagagacaaa ggttatgaaa
901 ctgagaaagt tggcacagca ggttgctaat tgccgccagt gtcttgaacg gtcaacagtc
961 ctcatcaacc aagctgagca tatcctgaaa gaaaatgacc aggcacggtt tctacagtct
1021 gcaaaaaata ttgctgagag ggtcgctatg gcaactgcat cttctcaagt tctgattcca
1081 gacatcaatt ttaatgatgc ctttgaaaac tttgctttag atttttccag agaaaagaaa
1141 ctgctagagg ggttagatta tttaacagcc ccaaacccac catctatccg agaagaactc
1201 tgtactgcct cccatgacac cattacagtc cactggatct cggatgatga gttcagcatc
1261 agctcctatg agcttcagta caccatattc actggccagg ctaacttcat cagcctgtat
1321 aattcagtag acagctggat gattgttccc aacattaaac agaaccatta cacagtgcat
1381 ggactccaga gcgggactcg ctacatcttc atcgttaaag ccataaacca agccggcagc
1441 cggaacagtg aacctacccg actaaaaaca aacagccaac cctttaaatt ggatcccaaa
1501 atgactcaca agaagttgaa gatctccaat gatggattgc agatggagaa ggatgaaagc
1561 tctctaaaga agagccacac cccagagagg tttagtggca cagggtgcta tggggcagca
1621 ggaaatatat tcattgacag tggctgccac tattgggagg tggtcatggg ttcctcaaca
1681 tggtatgcaa ttggcattgc ctacaaatca gctccaaaga atgaatggat tggcaagaat
1741 gcctcctcat gggtcttctc tcgctgcaat agtaacttcg tggtgagaca caacaacaag
1801 gaaatgctgg tggatgtgcc cccacacctg aagcgtctgg gtgtcctcct ggattatgac
1861 aacaatatgc tgtctttcta tgacccagct aactctctcc atcttcatac ttttgatgtg
1921 accttcattc ttccagtttg tccaacattt acaatctgga acaaatccct aatgatcctg
1981 tctggcttgc ctgccccaga ttttattgat taccctgagc ggcaggaatg caactgcagg
2041 cctcaagaat ccccttatgt ttctgggatg aaaacctgtc attaagtttc aggagagtat
2101 ataattcact ggctctctag ttcagcagtt cttcccctcc tacacctaag ttagcgttca
2161 atatacgaga cacaaaataa agtttgtttg aagcatccaa aataaaaaaa aaaaaaaa
//