LOCUS BC017554 1426 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens actin, alpha 2, smooth muscle, aorta, mRNA (cDNA clone
MGC:9221 IMAGE:3906861), complete cds.
ACCESSION BC017554
VERSION BC017554.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1426)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1426)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (19-NOV-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC017554.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 15 Row: a Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4501882.
FEATURES Location/Qualifiers
source 1..1426
/db_xref="H-InvDB:HIT000038044"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:9221 IMAGE:3906861"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1426
/gene="ACTA2"
/gene_synonym="ACTSA"
/db_xref="GeneID:59"
/db_xref="HGNC:HGNC:130"
/db_xref="MIM:102620"
CDS 55..1188
/gene="ACTA2"
/gene_synonym="ACTSA"
/codon_start=1
/product="actin, alpha 2, smooth muscle, aorta"
/protein_id="AAH17554.1"
/db_xref="GeneID:59"
/db_xref="HGNC:HGNC:130"
/db_xref="MIM:102620"
/translation="MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQG
VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEE
HPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDG
VTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKL
CYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGI
HETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP
PERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF"
BASE COUNT 424 a 361 c 327 g 314 t
ORIGIN
1 gtgtggagca gcccagccaa gcactgtcag gaatcctgtg aagcagctcc agctatgtgt
61 gaagaagagg acagcactgc cttggtgtgt gacaatggct ctgggctctg taaggccggc
121 tttgctgggg acgatgctcc cagggctgtt ttcccatcca ttgtgggacg tcccagacat
181 cagggggtga tggtgggaat gggacaaaaa gacagctacg tgggtgacga agcacagagc
241 aaaagaggaa tcctgaccct gaagtacccg atagaacatg gcatcatcac caactgggac
301 gacatggaaa agatctggca ccactctttc tacaatgagc ttcgtgttgc ccctgaagag
361 catcccaccc tgctcacgga ggcacccctg aaccccaagg ccaaccggga gaaaatgact
421 caaattatgt ttgagacttt caatgtccca gccatgtatg tggctatcca ggcggtgctg
481 tctctctatg cctctggacg cacaactggc atcgtgctgg actctggaga tggtgtcacc
541 cacaatgtcc ccatctatga gggctatgcc ttgccccatg ccatcatgcg tctggatctg
601 gctggccgag atctcactga ctacctcatg aagatcctga ctgagcgtgg ctattccttc
661 gttactactg ctgagcgtga gattgtccgg gacatcaagg agaaactgtg ttatgtagct
721 ctggactttg aaaatgagat ggccactgcc gcatcctcat cctcccttga gaagagttac
781 gagttgcctg atgggcaagt gatcaccatc ggaaatgaac gtttccgctg cccagagacc
841 ctgttccagc catccttcat cgggatggag tctgctggca tccatgaaac cacctacaac
901 agcatcatga agtgtgatat tgacatcagg aaggacctct atgctaacaa tgtcctatca
961 gggggcacca ctatgtaccc tggcattgcc gaccgaatgc agaaggagat cacggcccta
1021 gcacccagca ccatgaagat caagatcatt gcccctccgg agcgcaaata ctctgtctgg
1081 atcggtggct ccatcctggc ctctctgtcc accttccagc agatgtggat cagcaaacag
1141 gaatacgatg aagccgggcc ttccattgtc caccgcaaat gcttctaaaa cactttcctg
1201 ctcctctctg tctctagcac acaactgtga atgtcctgtg gaattatgcc ttcagttctt
1261 ttccaaatca ttcctagcca aagctctgac tcgttaccta tgtgtttttt aataaatctg
1321 aaataggaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1381 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa
//