LOCUS BC016743 1127 bp mRNA linear HUM 28-JUL-2005
DEFINITION Homo sapiens BRF1 homolog, subunit of RNA polymerase III
transcription initiation factor IIIB (S. cerevisiae), mRNA (cDNA
clone IMAGE:4830425), complete cds.
ACCESSION BC016743
VERSION BC016743.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1127)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1127)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-NOV-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC016743.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 34 Row: i Column: 6
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 22035555
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..1127
/db_xref="H-InvDB:HIT000037662"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4830425"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..1127
/gene="BRF1"
/gene_synonym="BRF"
/gene_synonym="hBRF"
/gene_synonym="TAFIII90"
/gene_synonym="TF3B90"
/gene_synonym="TFIIIB90"
/db_xref="GeneID:2972"
/db_xref="MIM:604902"
CDS 310..936
/gene="BRF1"
/gene_synonym="BRF"
/gene_synonym="hBRF"
/gene_synonym="TAFIII90"
/gene_synonym="TF3B90"
/gene_synonym="TFIIIB90"
/codon_start=1
/product="BRF1 protein"
/protein_id="AAH16743.1"
/db_xref="GeneID:2972"
/db_xref="MIM:604902"
/translation="MTGRVCRGCGGTDIELDAARGDAVCTACGSVLEDNIIVSEVQFV
ESSGGGSSAVGQFVSLDGAGKTPTLGGGFHVNLGKESRAQTLQNGRRHIHHLGNQLQL
NQHCLDTAFNFFKMAVSRHLTRGRKMAHVIAACLYLVCRTEGTPHMLLDLSDLLQVDS
LRPASFPTWGCDLGVVTRVVTGVYPRCLHASQWPVCAACPVRKFWSVG"
BASE COUNT 177 a 338 c 410 g 202 t
ORIGIN
1 aggtcgcccc tccgacgccg gcgcgccgca agggaggcca gctcgctcgc agtggggagg
61 tcgcggctcc agtcctcgcg tccccgccgt ggtcccggtg cctgtcccat cccgcgggcg
121 cggccgttgc gggcggcgcg gcccgggcgc gcgcggcgaa tctgcggctg cgaatcggct
181 ggagcggggc ctcgcgagag cggcgaggcc tgggcggctg ggcctgggcg ggcggcgcgg
241 gctgctccgg aggctcgggt ggcttgagag tcttgggagg ctccgggcgc tgcccgcgcg
301 gtcgccggca tgacgggccg cgtgtgccgc ggttgcggcg gcacggacat cgagctggac
361 gcggcgcgcg gggacgcggt gtgcaccgcc tgcggctcag tgctggagga caacatcatc
421 gtgtccgagg tgcagttcgt ggagagcagc ggcggcggct cctcggccgt gggccagttc
481 gtgtccctgg acggtgctgg caaaaccccg actctgggtg gcggcttcca cgtgaatctg
541 gggaaggagt cgagagcgca gaccctgcag aatgggaggc gccacatcca ccacctgggg
601 aaccagctgc agctgaacca gcactgcctg gacaccgcct tcaacttctt caagatggcc
661 gtgagcaggc acctgacccg cggccggaag atggcccacg tgattgctgc ctgcctctac
721 ctggtctgcc gtacggaggg cacgccgcac atgctcctgg acctcagcga cctgctccag
781 gtagacagcc tccgtcctgc atctttcccc acctggggtt gtgacctggg ggttgtgacc
841 agggttgtga ccggggtgta ccccaggtgc ctccacgcat ctcagtggcc ggtctgtgct
901 gcctgcccgg tcaggaagtt ttggtctgta ggatgagggg ccttcatgac atgggggtca
961 cagtccgaga cttgtgggag tgtgggtctc catggcagga gggcacctgc caatgctggg
1021 cacggttggg tgttagctag gcctgggtga acagtgaact tttgtcctac acaaaggaga
1081 ttattttcaa ttaaaaaatt tttttgtgag caaaaaaaaa aaaaaaa
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