LOCUS BC016055 2030 bp mRNA linear HUM 09-DEC-2005
DEFINITION Homo sapiens nucleoporin 50kDa, mRNA (cDNA clone MGC:23022
IMAGE:4856954), complete cds.
ACCESSION BC016055
VERSION BC016055.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2030)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2030)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (22-OCT-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 34 Row: e Column: 6
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 24497450.
FEATURES Location/Qualifiers
source 1..2030
/db_xref="H-InvDB:HIT000088916"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:23022 IMAGE:4856954"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_15"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2030
/gene="NUP50"
/gene_synonym="MGC39961"
/gene_synonym="NPAP60"
/db_xref="GeneID:10762"
/db_xref="HGNC:HGNC:8065"
/db_xref="IMGT/GENE-DB:8065"
/db_xref="MIM:604646"
CDS 482..1804
/gene="NUP50"
/gene_synonym="MGC39961"
/gene_synonym="NPAP60"
/codon_start=1
/product="NUP50 protein"
/protein_id="AAH16055.1"
/db_xref="GeneID:10762"
/db_xref="HGNC:HGNC:8065"
/db_xref="IMGT/GENE-DB:8065"
/db_xref="MIM:604646"
/translation="MASEEVLKNRAIKKAKRRNVGFESDTGGAFKGFKGLVVPSGGGR
FSGFGSGAGGKPLEGLSNGNNITSAPPFASAKAAADPKVAFGSLAANGPTTLVDKVSN
PKTNGDSQQPSSSGLASSKACVGNAYHKQLAALNCSVRDWIVKHVNTNPLCDLTPIFK
DYEKYLANIEQQHGNSGRNSESESNKVAAETQSPSLFGSTKLQQESTFLFHGNKTEDT
PDKKMEVASEKKTDPSSLGATSASFNFGKKVDSSVLGSLSSVPLTGFSFSPGNSSLFG
KDTTQSKPVSSPFPTKPLEGQAEGDSGECKGGDEEENDEPPKVVVTEVKEEDAFYSKK
CKLFYKKDNEFKEKGIGTLHLKPTANQKTQLLVRADTNLGNILLNVLIPPNMPCTRTG
KNNVLIVCVPNPPIDEKNATMPVTMLIRVKTSEDADELHKILLEKKDA"
BASE COUNT 642 a 451 c 487 g 450 t
ORIGIN
1 cggcgctctc gcctccctgc cgaacacagc gtgaggagcc cccccaggga catggtgttt
61 gagtctctgg gcttgccgag cactaagtcc tctgagttcc gcagcgcagc accggaagcg
121 gccgagcgcg ctcagcccgg cgacccctgc gggctccaga cccctgcgcc gctgcgcccc
181 gggtttcgcc gcaaccaaga cccagcgagt gcagcggcgg ccgccgagga ggttcgaaaa
241 catggccaaa agaaatgccg agaaggaact gacagatagg aattgggatc aagaagatga
301 agctgaagag ggtaaaagaa gggctataaa ctaaagccga agatattcag cagatttctg
361 acgtggacat cagaagtgtg gacatgagtg tgctttgagc gcactgtgga agagcagtgg
421 ttgtccagca aggtcctagg tgcctccgtg gcctttcgta cagtgggtgg gaacattctc
481 catggccagt gaggaagtct tgaagaatag agccataaag aaagcaaagc gcagaaatgt
541 tggatttgaa tctgacactg gaggagcctt taaaggtttt aaaggtttgg ttgtaccttc
601 tggaggagga cgcttttctg gatttggtag tggcgctgga gggaagcctt tggaaggact
661 gtcgaatgga aacaacataa ccagtgcccc tcccttcgcc agtgcaaagg cagcggcaga
721 tcccaaggta gcctttggtt ctcttgctgc aaatggccct accaccttgg ttgataaagt
781 ttcaaatccc aaaactaatg gggacagtca gcagccctcc tcctctggcc ttgcttccag
841 taaagcttgt gtcggaaatg cctatcacaa gcagttggcc gccttgaact gctccgtgcg
901 ggattggata gtgaagcacg tgaatacaaa ccccctctgt gatctgacac ctatctttaa
961 agactatgag aaatatttag caaacattga acagcaacac gggaacagtg gcaggaattc
1021 tgaaagtgaa tctaacaaag tggcagctga aacacagtct ccttcccttt ttggctcaac
1081 aaaattacag caagagtcaa cgtttttgtt tcatggcaac aaaactgaag atacacctga
1141 caagaagatg gaggtggcat ctgaaaagaa aacggaccca tcatcactag gagcgacaag
1201 tgcctcattt aatttcggca agaaagttga tagctctgtt ttgggctcat taagctctgt
1261 ccccctgact ggattttctt tctcccctgg aaactccagt ttatttggca aagatactac
1321 ccagagtaaa ccagtctctt caccatttcc cactaaacca ttggagggcc aagcagaagg
1381 tgacagtggt gaatgcaaag gtggagatga agaagagaat gatgagccac ccaaagtagt
1441 agttaccgaa gtaaaagaag aagacgcttt ttactccaaa aagtgtaaac tgttttacaa
1501 gaaagacaat gagtttaaag agaaaggcat aggtactctg catttaaaac ctacagcaaa
1561 tcagaagaca cagcttttgg tgcgggcaga caccaattta ggcaacatat tgctgaacgt
1621 tctgattcca cccaatatgc catgtacgcg aacagggaag aataacgttc ttatcgtctg
1681 tgttccaaat ccaccaattg acgagaagaa tgccaccatg ccagtcacca tgttgattcg
1741 ggtaaaaacc agcgaggatg cagacgagtt gcacaaaatt ttactggaga aaaaggatgc
1801 ctgaacacgc aaagtcggct gcagaattat tgccaagttg ctgctgcttc caccgcccct
1861 tagtcagttt ttcttctctt ctttgacatt ctaagaactt atagataact taaaactttt
1921 gtgaggaaga ttaatgtagc caataaaacc tttaaatgtt aaaaaaaaaa aaaaaaaaaa
1981 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
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