LOCUS BC015742 2231 bp mRNA linear HUM 06-OCT-2003
DEFINITION Homo sapiens polymerase (DNA directed), eta, mRNA (cDNA clone
MGC:23030 IMAGE:4872554), complete cds.
ACCESSION BC015742
VERSION BC015742.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2231)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2231)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steven Jones, Jennifer Asano, Ian Bosdet, Yaron Butterfield,
Susanna Chan, Readman Chiu, Chris Fjell, Erin Garland, Ran Guin,
Letticia Hsiao, Martin Krzywinski, Reta Kutsche, Oliver Lee, Soo
Sen Lee, Victor Ling, Carrie Mathewson, Candice McLeavy, Steven
Ness, Pawan Pandoh, Anna-Liisa Prabhu, Parvaneh Saeedi, Jacqueline
Schein, Duane Smailus, Michael Smith, Lorraine Spence, Jeff Stott,
Michael Thorne, Miranada Tsai, Natasja van den Bosch, Jill Vardy,
George Yang, Scott Zuyderduyn, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 34 Row: l Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 5729981.
FEATURES Location/Qualifiers
source 1..2231
/db_xref="H-InvDB:HIT000037290"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:23030 IMAGE:4872554"
/tissue_type="Skin, melanotic melanoma, high MDR."
/clone_lib="NIH_MGC_49"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2231
/gene="POLH"
/gene_synonym="RAD30A"
/gene_synonym="XP-V"
/gene_synonym="XPV"
/db_xref="GeneID:5429"
/db_xref="MIM:603968"
CDS 149..1393
/gene="POLH"
/gene_synonym="RAD30A"
/gene_synonym="XP-V"
/gene_synonym="XPV"
/codon_start=1
/product="POLH protein"
/protein_id="AAH15742.1"
/db_xref="GeneID:5429"
/db_xref="MIM:603968"
/translation="MATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKG
GGIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVMEI
MSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYIEGLPQGPTTAEET
VQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAAIERETGFQCSAGISH
NKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEILGIEYMG
ELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATR
EQVQWWLLQLAQELEERLTKDRNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDA
HKMSHDAFTVIKNCNTSGIQTE"
misc_feature 167..1126
/gene="POLH"
/gene_synonym="RAD30A"
/gene_synonym="XP-V"
/gene_synonym="XPV"
/note="DinP; Region: Nucleotidyltransferase/DNA polymerase
involved in DNA repair [DNA replication, recombination,
and repair]"
/db_xref="CDD:COG0389"
BASE COUNT 665 a 537 c 519 g 510 t
ORIGIN
1 ataaatctcg ctcgaaactc actggaccgc tcctagaaag gcgaaaagat attcaggagc
61 ccttccattt tccttccagt aggcaccgaa cccagcattt tcggcaaccg ctgctggcag
121 ttttgccagg tgtttgttac cttgaaaaat ggctactgga caggatcgag tggttgctct
181 cgtggacatg gactgttttt ttgttcaagt ggagcagcgg caaaatcctc atttgaggaa
241 taaaccttgt gcagttgtac agtacaaatc atggaagggt ggtggaataa ttgcagtgag
301 ttatgaagct cgtgcatttg gagtcactag aagtatgtgg gcagatgatg ctaagaagtt
361 atgtccagat cttctactgg cacaagttcg tgagtcccgt gggaaagcta acctcaccaa
421 gtaccgggaa gccagtgttg aagtgatgga gataatgtct cgttttgctg tgattgaacg
481 tgccagcatt gatgaggctt acgtagatct gaccagtgct gtacaagaga gactacaaaa
541 gctacaaggt cagcctatct cggcagactt gttgccaagc acttacattg aagggttgcc
601 ccaaggccct acaacggcag aagagactgt tcagaaagag gggatgcgaa aacaaggctt
661 atttcaatgg ctcgattctc ttcagattga taacctcacc tctccagacc tgcagctcac
721 cgtgggagca gtgattgtgg aggaaatgag agcagccata gagagggaga ctggttttca
781 gtgttcagct ggaatttcac acaataaggt cctggcaaaa ctggcctgtg gactaaacaa
841 gcccaaccgc caaaccctgg tttcacatgg gtcagtccca cagctcttca gccaaatgcc
901 cattcgcaaa atccgtagtc ttggaggaaa gctaggggcc tctgtcattg agatcctagg
961 gatagaatac atgggtgaac tgacccagtt cactgaatcc cagctccaga gtcattttgg
1021 ggagaagaat gggtcttggc tatatgccat gtgccgaggg attgaacatg atccagttaa
1081 acccaggcaa ctacccaaaa ccattggctg tagtaagaac ttcccaggaa aaacagctct
1141 tgctactcgg gaacaggtac aatggtggct gttgcaatta gcccaggaac tagaggagag
1201 actgactaaa gaccgaaatg ataatgacag ggtagccacc cagctggttg tgagcattcg
1261 tgtacaagga gacaaacgcc tcagcagcct gcgccgctgc tgtgccctta cccgctatga
1321 tgctcacaag atgagccatg atgcatttac tgtcatcaag aactgtaata cttctggaat
1381 ccagacagaa tgacatcacc agcttcttga gcagtgaccc aagttctctg ccaaaggtgc
1441 cagttaccag ctcagaagct aagacccagg gaagtggccc agcggtgaca gccactaaga
1501 aagcaaccac gtctctggaa tcattcttcc aaaaagctgc agaaaggcag aaagttaaag
1561 aagcttcgct ttcatctctt actgctccca ctcaggctcc catgagcaat tcaccatcca
1621 agccctcatt accttttcaa accagtcaaa gtacaggaac tgagcccttc tttaagcaga
1681 aaagtctgct tctaaagcag aaacagctta ataattcttc agtttcttcc ccccaacaaa
1741 acccatggtc caactgtaaa gcattaccaa actctttacc aacagagtat ccagggtgtg
1801 tccctgtttg tgaaggggtg tcgaagctag aagaatcctc taaagcaact cctgcagaga
1861 tggatttggc ccacaacagc caaagcatgc acgcctcttc agcttccaaa tctgtgctgg
1921 aggtgactca gaaagcaacc ccaaatccaa gtcttctagc tgctgaggac caagtgccct
1981 gtgagaagtg tggctccctg gtaccggtat gggatatgcc agaacacatg gactatcatt
2041 ttgcattgga gttgcagaaa tcctttttgc agccccactc ttcaaacccc caggttgttt
2101 ctgccgtatc tcatcaaggc aaaagaaatc ccaagagccc tttggcctgc actaataaac
2161 gccccaggcc tgagggcatg caaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2221 aaaaaaaaaa a
//