LOCUS BC015025 4487 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens NCK-associated protein 1, mRNA (cDNA clone MGC:8981
IMAGE:3907646), complete cds.
ACCESSION BC015025
VERSION BC015025.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4487)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4487)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-OCT-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 15 Row: c Column: 2
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 45545410.
FEATURES Location/Qualifiers
source 1..4487
/db_xref="H-InvDB:HIT000036967"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8981 IMAGE:3907646"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4487
/gene="NCKAP1"
/gene_synonym="HEM2"
/gene_synonym="KIAA0587"
/gene_synonym="MGC8981"
/gene_synonym="NAP1"
/gene_synonym="NAP125"
/db_xref="GeneID:10787"
/db_xref="HGNC:HGNC:7666"
/db_xref="MIM:604891"
CDS 272..3658
/gene="NCKAP1"
/gene_synonym="HEM2"
/gene_synonym="KIAA0587"
/gene_synonym="MGC8981"
/gene_synonym="NAP1"
/gene_synonym="NAP125"
/codon_start=1
/product="NCK-associated protein 1"
/protein_id="AAH15025.1"
/db_xref="GeneID:10787"
/db_xref="HGNC:HGNC:7666"
/db_xref="MIM:604891"
/translation="MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAK
PSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVME
FKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLY
NYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRN
LSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTD
ATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSH
AGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNM
PKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLS
VCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLAD
HRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYS
IAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTL
SDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTAL
SELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELL
TSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLR
QVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLM
WHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVIL
SFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEID
PALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIH
CLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLL
DMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA"
BASE COUNT 1375 a 864 c 933 g 1315 t
ORIGIN
1 ggagagcgcc gggggtgggg gcggaagggc ggcgggcgga aggcaggagg ctgccggggc
61 gcgggctgct gcgggagaag gggctccgag gagtccgccg cggctcgctc tgtcgccggc
121 gcgggattgg ggcgcgaggg ccatgggcgc gctctcctaa ggcggaggtc gcgggcggga
181 ggggaggagg cccgagagag gctgctgcga aggccgcggg cccgtgactg ggcgcgaggc
241 ggccggcggc ggcggcggca ccagcaccac catgtcgcgc tcagtgctgc agcccagtca
301 gcagaagctg gcggagaagc tcaccatcct caacgaccgg ggcgtcggca tgctcacccg
361 cctctacaac atcaagaagg catgtggaga ccccaaggca aaaccatcct atcttatcga
421 caaaaacctg gaatctgctg tgaaattcat agtcagaaaa ttccctgctg tagaaacccg
481 caacaacaat caacagcttg cacaactaca gaaagaaaaa tcagagattc tgaaaaatct
541 ggcattatat tacttcacat ttgtagatgt tatggaattt aaggaccatg tttgtgaatt
601 gctgaatact attgacgttt gccaagtctt ctttgatatt actgtaaact ttgatttaac
661 aaagaactac ttagatttaa ttataaccta tacaacacta atgatactgc tgtctcgaat
721 tgaagaaagg aaggcaatca ttggattata caactatgcc catgaaatga ctcatggagc
781 aagtgacaga gaatacccac gccttggcca gatgattgtg gattatgaaa accctttaaa
841 gaagatgatg gaagaatttg taccccatag caagtctctt tcagatgcac taatttctct
901 tcaaatggta tatcctcgaa ggaatctttc agctgaccag tggagaaatg cccagttatt
961 gagcctcatc agtgcaccta gtacaatgct taatccagca cagtccgaca ctatgccttg
1021 tgaatacctc tctttggatg caatggaaaa gtggattatc tttggcttta ttttgtgcca
1081 tgggatccta aatactgacg ctacagcact gaacctttgg aaactagctc ttcaaagtag
1141 ctcttgcctc tctctctttc gggatgaagt tttccacatt cacaaagctg cagaagactt
1201 atttgtaaac atacgaggct ataataaacg tattaatgac ataagagaat gcaaggaggc
1261 agccgtgtca catgctggtt caatgcacag agaaagacgc aagtttttaa gatctgcact
1321 gaaggaattg gctactgtcc tctctgatca acctggattg ctaggtccca aggcactttt
1381 tgtttttatg gcattatcct ttgcccgtga tgaaatcatc tggctacttc gtcatgcaga
1441 taacatgcca aagaagagtg cagacgactt tatagataag cacattgctg aattaatatt
1501 ttacatggaa gaacttagag cacatgtgag gaaatacgga cctgtaatgc agaggtatta
1561 cgtgcagtac ctttctggct ttgatgctgt tgtcctcaat gaactcgtgc agaatctttc
1621 tgtttgccct gaagatgaat caatcatcat gtcctctttt gttaacacta tgacttccct
1681 aagtgtaaaa caagttgaag atggggaagt atttgatttc agaggaatga gattagattg
1741 gtttaggtta caggcatata ctagtgtctc aaaggcttca cttggccttg cagatcacag
1801 agaacttgga aagatgatga atacaataat ttttcataca aaaatggtag attccttggt
1861 ggaaatgttg gtggaaacat cagatctctc catattttgt ttttatagtc gtgcttttga
1921 gaagatgttt caacagtgtt tggagttacc ctctcaatca agatactcaa ttgcatttcc
1981 actactttgc actcatttta tgagttgcac gcatgaacta tgtccagaag agcgacatca
2041 tattggagat cgcagtcttt ccttatgtaa tatgttccta gatgaaatgg ccaaacaagc
2101 tcgaaatctc atcactgata tttgcacaga acagtgtacc cttagtgacc agttgctacc
2161 caagcattgt gccaaaacta tcagtcaagc agtgaataag aaatcaaaaa agcagactgg
2221 taagaaaggg gaacctgaaa gggagaaacc aggtgttgag agcatgagga aaaacaggct
2281 ggttgtgacc aaccttgata aattgcacac tgcactttct gagttatgct tctctataaa
2341 ttatgtacca aacatggtgg tatgggaaca tacctttacc ccacgagaat atttgacttc
2401 tcatctggaa atacgcttta ccaagtcaat tgttgggatg actatgtata atcaagccac
2461 acaggaaatt gcaaaacctt cagaacttct aacaagtgta agagcataca tgaccgtact
2521 ccagtcaata gaaaactatg tgcagattga tattacaaga gtatttaata atgtgcttct
2581 tcaacaaaca caacatttag acagtcatgg agagccaacc attacaagtc tatacacaaa
2641 ttggtatttg gaaactttgt tacgacaagt cagcaatggc catatagcat attttcctgc
2701 aatgaaagcg tttgtgaact tacctacaga aaatgaatta acattcaatg cagaggaata
2761 ttctgacata tcagaaatga ggtcattatc agaactacta ggcccatatg gtatgaagtt
2821 tctaagtgaa agccttatgt ggcatatttc atcacaagtt gctgaactta agaaacttgt
2881 ggtggagaat gttgatgtgt taacacaaat gaggaccagc tttgacaaac cagaccagat
2941 ggctgcactg tttaaaagat tatcatctgt tgacagtgtc ttgaagagga tgacaataat
3001 tggtgtaatt ttatccttcc gatcattggc acaagaagca cttagagatg tcttatccta
3061 ccacattcct tttcttgtaa gttcaattga agattttaag gatcacattc caagggaaac
3121 tgatatgaag gttgcaatga atgtgtatga gttatcatca gctgccggat taccttgtga
3181 gattgatcct gcattggtcg tagctctttc ttcacaaaaa tcggaaaaca ttagtccaga
3241 agaagagtat aaaattgcct gccttctcat ggtgtttgtg gcagtttctt tgccaacact
3301 ggccagtaat gtgatgtctc agtacagccc tgctatagaa gggcattgca acaacataca
3361 ttgcttggcc aaagccatca accagattgc tgcagctttg tttacaattc acaaaggaag
3421 cattgaagac cgtcttaaag aatttctggc gcttgcatcc tccagtctac tgaaaattgg
3481 ccaggagaca gataaaacta caacaagaaa tagagaatct gtttatttac tgctagatat
3541 gattgtacaa gaatctccat tccttacaat ggatcttttg gaatcttgtt ttccttatgt
3601 cttgctgaga aatgcatacc atgctgtcta caaacaaagt gttacatctt ctgcataaaa
3661 ttacctactt aatcaagata agcacgcatt tttgttgcct tggttttacc tgtagactgt
3721 ggaactattt taccttaaga cctgaaaaag ttttgtggat tataaatttc tttcatacgg
3781 ttgtattttc tgatcattgg tttcttaata tggttgtact acagtatact tggttgattt
3841 aggttgcaca ttcactgaat tcactgagat tattcctata attttaaagt atcatttatt
3901 tgaaaaacat acattatcaa catgtttttg atatttgata atgaaaaaaa tctttgcttg
3961 tttatttctg aaaaagaact gtatttagtg attattttag atagtgatat tatagtattc
4021 atctgtgtgt aaattatttc atatagggaa gagttctgat ctgtacctat ggttcttatt
4081 gaaaacaaca ttggatgtgc atttctgtga tgttatgaat acatttctac tttattttga
4141 aacatttgcc aaactaaata ctgtaacact gtataacatt taaaaatgtt aaagaactgc
4201 ttagtattag aagcagatca tttcccaaaa ttctaagagc agcagcatat gttgttgctt
4261 gtataaagcc tagcgataat ttttagacta acttccatgg tgccctgttg gcattagcac
4321 taccattgta ccctgctgta taataaacaa tcttagacat ttatcaactg ttgatacaaa
4381 tgttagtccc taaccacttt ttatatatgt tttaaatttt tgaaattcaa gtgtacctgc
4441 cataacataa aataaacact agactgtatc acaaaaaaaa aaaaaaa
//