LOCUS BC014504 1690 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens prolyl 4-hydroxylase, beta polypeptide, mRNA (cDNA
clone IMAGE:4861217), partial cds.
ACCESSION BC014504
VERSION BC014504.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1690)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1690)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (17-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 34 Row: g Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1690
/db_xref="H-InvDB:HIT000036744"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4861217"
/tissue_type="Skin, melanotic melanoma, high MDR."
/clone_lib="NIH_MGC_49"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..1690
/gene="P4HB"
/gene_synonym="DSI"
/gene_synonym="GIT"
/gene_synonym="P4Hbeta"
/gene_synonym="PDI"
/gene_synonym="PDIA1"
/gene_synonym="PHDB"
/gene_synonym="PO4HB"
/gene_synonym="PROHB"
/db_xref="GeneID:5034"
/db_xref="HGNC:HGNC:8548"
/db_xref="MIM:176790"
CDS <1..823
/gene="P4HB"
/gene_synonym="DSI"
/gene_synonym="GIT"
/gene_synonym="P4Hbeta"
/gene_synonym="PDI"
/gene_synonym="PDIA1"
/gene_synonym="PHDB"
/gene_synonym="PO4HB"
/gene_synonym="PROHB"
/codon_start=2
/product="P4HB protein"
/protein_id="AAH14504.2"
/db_xref="GeneID:5034"
/db_xref="HGNC:HGNC:8548"
/db_xref="MIM:176790"
/translation="FEGEVTKENLLDFIKHNQLPLVIEFTEQSVSDYDGKLSNFKTAA
ESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAE
WITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAP
WCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRT
VIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL"
BASE COUNT 403 a 457 c 469 g 361 t
ORIGIN
1 ctttgaaggg gaggtcacca aggagaacct gctggacttt atcaaacaca accagctgcc
61 ccttgtcatt gagttcaccg agcagagtgt gtctgactat gacggcaaac tgagcaactt
121 caaaacagca gccgagagct tcaagggcaa gatcctgttc atcttcatcg acagcgacca
181 caccgacaac cagcgcatcc tcgagttctt tggcctgaag aaggaagagt gcccggccgt
241 gcgcctcatc accctggagg aggagatgac caagtacaag cccgaatcgg aggagctgac
301 ggcagagtgg atcacagagt tctgccaccg cttcctggag ggcaaaatca agccccacct
361 gatgagccag gagctgccgg aggactggga caagcagcct gtcaaggtgc ttgttgggaa
421 gaactttgaa gacgtggctt ttgatgagaa aaaaaacgtc tttgtggagt tctatgcccc
481 atggtgtggt cactgcaaac agttggctcc catttgggat aaactgggag agacgtacaa
541 ggaccatgag aacatcgtca tcgccaagat ggactcgact gccaacgagg tggaggccgt
601 caaagtgcac agcttcccca cactcaagtt ctttcctgcc agtgccgaca ggacggtcat
661 cgattacaac ggggaacgca cgctggatgg ttttaagaaa ttcctggaga gcggtggcca
721 ggatggggca ggggatgatg acgatctcga ggacctggaa gaagcagagg agccagacat
781 ggaggaagac gatgatcaga aagctgtgaa agatgaactg taatacgcaa agccagaccc
841 gggcgctgcc gagacccctc gggggctgca cacccagcag cagcgcacgc ctccgaagcc
901 tgcggcctcg tttgaaggag ggcgtcgccg gaaacccagg gaacctctct gaagtgacac
961 ctcaccccta cacaccgtcc gttcaccccc gtctcttcct tctgcttttc ggtttttgga
1021 aagggatcca tctccaggca gcccaccctg gtggggcttg tttcctgaaa ccatgatgta
1081 ctttttcata catgagtctg tccagagtgc ttgctaccgt gttcggagtc tcgctgcctc
1141 cctcccgcgg gaggtttctc ctctttttga aaattccgtc tgtgggattt ttagacattt
1201 ttcgacatca gggtatttgt tccaccttgg ccaggcctcc tcggagaagc ttgtcccccg
1261 tgtgggaggg acggagccgg actggacatg gtcactcagt accgcctgca gtgtcgccat
1321 gactgatcat ggctcttgca tttttgggta aatggagact tccggatcct gtcagggtgt
1381 cccccatgcc tggaagagga gctggtggct gccagccctg ggtcccggca caggcctggg
1441 ccttcccctt ccctcaagcc agggctcctc ctcctgtcgt gggctcattg tgaccactgg
1501 cctctctaca gcacggcctg tggcctgttc aaggcagaac cacgaccctt gactcccggg
1561 tggggaggtg gccaaggatg ctggagctga atcagacgct gacagttctt caggcatttc
1621 tatttcacaa tcgaattgaa cacattggcc aaataaagtt gaaattttaa aaaaaaaaaa
1681 aaaaaaaaaa
//