LOCUS BC014453 1864 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens beta-site APP-cleaving enzyme 2, mRNA (cDNA clone
MGC:23029 IMAGE:4868925), complete cds.
ACCESSION BC014453
VERSION BC014453.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1864)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1864)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (17-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 34 Row: l Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21040358.
FEATURES Location/Qualifiers
source 1..1864
/db_xref="H-InvDB:HIT000036709"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:23029 IMAGE:4868925"
/tissue_type="Skin, melanotic melanoma, high MDR."
/clone_lib="NIH_MGC_49"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1864
/gene="BACE2"
/gene_synonym="ALP56"
/gene_synonym="ASP1"
/gene_synonym="ASP21"
/gene_synonym="BAE2"
/gene_synonym="CDA13"
/gene_synonym="CEAP1"
/gene_synonym="DRAP"
/db_xref="GeneID:25825"
/db_xref="HGNC:HGNC:934"
/db_xref="MIM:605668"
CDS 92..1648
/gene="BACE2"
/gene_synonym="ALP56"
/gene_synonym="ASP1"
/gene_synonym="ASP21"
/gene_synonym="BAE2"
/gene_synonym="CDA13"
/gene_synonym="CEAP1"
/gene_synonym="DRAP"
/codon_start=1
/product="beta-site APP-cleaving enzyme 2"
/protein_id="AAH14453.1"
/db_xref="GeneID:25825"
/db_xref="HGNC:HGNC:934"
/db_xref="MIM:605668"
/translation="MGALARALLLPLLAQWLLRAAPELAPAPFTLPLRVAAATNRVVA
PTPGPGTPAERHADGLALALEPALASPAGAANFLAMVDNLQGDSGRGYYLEMLIGTPP
QKLQILVDTGSSNFAVAGTPHSYIDTYFDTERSSTYRSKGFDVTVKYTQGSWTGFVGE
DLVTIPKGFNTSFLVNIATIFESENFFLPGIKWNGILGLAYATLAKPSSSLETFFDSL
VTQANIPNVFSMQMCGAGLPVAGSGTNGGSLVLGGIEPSLYKGDIWYTPIKEEWYYQI
EILKLEIGGQSLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEAVARASLIPEFSD
GFWTGSQLACWTNSETPWSYFPKISIYLRDENSSRSFRITILPQLYIQPMMGAGLNYE
CYRFGISPSTNALVIGATVMEGFYVIFDRAQKRVGFAASPCAEIAGAAVSEISGPFST
EDVASNCVPAQSLSEPILWIVSYALMSVCGAILLVLIVLLLLPFRCQRRPRDPEVVND
ESSLVRHRWK"
BASE COUNT 385 a 554 c 498 g 427 t
ORIGIN
1 gccccgcgcg ccggccgagt cgctgagccg cggctgccgg acgggacggg accggctagg
61 ctgggcgcgc cccccgggcc ccgccgtggg catgggcgca ctggcccggg cgctgctgct
121 gcctctgctg gcccagtggc tcctgcgcgc cgccccggag ctggcccccg cgcccttcac
181 gctgcccctc cgggtggccg cggccacgaa ccgcgtagtt gcgcccaccc cgggacccgg
241 gacccctgcc gagcgccacg ccgacggctt ggcgctcgcc ctggagcctg ccctggcgtc
301 ccccgcgggc gccgccaact tcttggccat ggtagacaac ctgcaggggg actctggccg
361 cggctactac ctggagatgc tgatcgggac ccccccgcag aagctacaga ttctcgttga
421 cactggaagc agtaactttg ccgtggcagg aaccccgcac tcctacatag acacgtactt
481 tgacacagag aggtctagca cataccgctc caagggcttt gacgtcacag tgaagtacac
541 acaaggaagc tggacgggct tcgttgggga agacctcgtc accatcccca aaggcttcaa
601 tacttctttt cttgtcaaca ttgccactat ttttgaatca gagaatttct ttttgcctgg
661 gattaaatgg aatggaatac ttggcctagc ttatgccaca cttgccaagc catcaagttc
721 tctggagacc ttcttcgact ccctggtgac acaagcaaac atccccaacg ttttctccat
781 gcagatgtgt ggagccggct tgcccgttgc tggatctggg accaacggag gtagtcttgt
841 cttgggtgga attgaaccaa gtttgtataa aggagacatc tggtataccc ctattaagga
901 agagtggtac taccagatag aaattctgaa attggaaatt ggaggccaaa gccttaatct
961 ggactgcaga gagtataacg cagacaaggc catcgtggac agtggcacca cgctgctgcg
1021 cctgccccag aaggtgtttg atgcggtggt ggaagctgtg gcccgcgcat ctctgattcc
1081 agaattctct gatggtttct ggactgggtc ccagctggcg tgctggacga attcggaaac
1141 accttggtct tacttcccta aaatctccat ctacctgaga gatgagaact ccagcaggtc
1201 attccgtatc acaatcctgc ctcagcttta cattcagccc atgatggggg ccggcctgaa
1261 ttatgaatgt taccgattcg gcatttcccc atccacaaat gcgctggtga tcggtgccac
1321 ggtgatggag ggcttctacg tcatcttcga cagagcccag aagagggtgg gcttcgcagc
1381 gagcccctgt gcagaaattg caggtgctgc agtgtctgaa atttccgggc ctttctcaac
1441 agaggatgta gccagcaact gtgtccccgc tcagtctttg agcgagccca ttttgtggat
1501 tgtgtcctat gcgctcatga gcgtctgtgg agccatcctc cttgtcttaa tcgtcctgct
1561 gctgctgccg ttccggtgtc agcgtcgccc ccgtgaccct gaggtcgtca atgatgagtc
1621 ctctctggtc agacatcgct ggaaatgaat agccaggcct gacctcaagc aaccatgaac
1681 tcagctatta agaaaatcac atttccaggg cagcagccgg gatcgatggt ggcgctttct
1741 cctgtgccca cccgtcttca atctctgttc tgctcccaga tgccttctag attcactgtc
1801 ttttgattct tgattttcaa gctttcaaat cctccctact tccaagaaaa aaaaaaaaaa
1861 aaaa
//