LOCUS BC014074 2067 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens preferentially expressed antigen in melanoma, mRNA
(cDNA clone MGC:20036 IMAGE:3954524), complete cds.
ACCESSION BC014074
VERSION BC014074.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2067)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2067)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC014074.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 28 Row: h Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 46249368.
FEATURES Location/Qualifiers
source 1..2067
/db_xref="H-InvDB:HIT000036502"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20036 IMAGE:3954524"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2067
/gene="PRAME"
/gene_synonym="OIP4"
/db_xref="GeneID:23532"
/db_xref="HGNC:HGNC:9336"
/db_xref="MIM:606021"
CDS 159..1688
/gene="PRAME"
/gene_synonym="OIP4"
/codon_start=1
/product="preferentially expressed antigen in melanoma"
/protein_id="AAH14074.1"
/db_xref="GeneID:23532"
/db_xref="HGNC:HGNC:9336"
/db_xref="MIM:606021"
/translation="MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAA
LELLPRELFPPLFMAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLD
GLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRK
VDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVKRKKNVLRLCCKKLKIFAM
PMQDIKMILKMVQLDSIEDLEVTCTWKLPTLAKFSPYLGQMINLRRLLLSHIHASSYI
SPEKEEQYIAQFTSQFLSLQCLQALYVDSLFFLRGRLDQLLRHVMNPLETLSITNCRL
SEGDVMHLSQSPSVSQLSVLSLSGVMLTDVSPEPLQALLERASATLQDLVFDECGITD
DQLLALLPSLSHCSQLTTLSFYGNSISISALQSLLQHLIGLSNLTHVLYPVPLESYED
IHGTLHLERLAYLHARLRELLCELGRPSMVWLSANPCPHCGDRTFYDPEPILCPCFMP
N"
BASE COUNT 521 a 510 c 547 g 489 t
ORIGIN
1 gggaaaccga ctcctgggag cagggaggaa cgcgcgctcc agagacaact tcgcggtgtg
61 gtgaactctc tgaggaaaaa cacgtgcgtg gcaacaagtg actgagacct agaaatccaa
121 gcgttggagg tcctgaggcc agcctaagtc gcttcaaaat ggaacgaagg cgtttgtggg
181 gttccattca gagccgatac atcagcatga gtgtgtggac aagcccacgg agacttgtgg
241 agctggcagg gcagagcctg ctgaaggatg aggccctggc cattgccgcc ctggagttgc
301 tgcccaggga gctcttcccg ccactcttca tggcagcctt tgacgggaga cacagccaga
361 ccctgaaggc aatggtgcag gcctggccct tcacctgcct ccctctggga gtgctgatga
421 agggacaaca tcttcacctg gagaccttca aagctgtgct tgatggactt gatgtgctcc
481 ttgcccagga ggttcgcccc aggaggtgga aacttcaagt gctggattta cggaagaact
541 ctcatcagga cttctggact gtatggtctg gaaacagggc cagtctgtac tcatttccag
601 agccagaagc agctcagccc atgacaaaga agcgaaaagt agatggtttg agcacagagg
661 cagagcagcc cttcattcca gtagaggtgc tcgtagacct gttcctcaag gaaggtgcct
721 gtgatgaatt gttctcctac ctcattgaga aagtgaagcg aaagaaaaat gtactacgcc
781 tgtgctgtaa gaagctgaag atttttgcaa tgcccatgca ggatatcaag atgatcctga
841 aaatggtgca gctggactct attgaagatt tggaagtgac ttgtacctgg aagctaccca
901 ccttggcgaa attttctcct tacctgggcc agatgattaa tctgcgtaga ctcctcctct
961 cccacatcca tgcatcttcc tacatttccc cggagaagga agagcagtat atcgcccagt
1021 tcacctctca gttcctcagt ctgcagtgcc tgcaggctct ctatgtggac tctttatttt
1081 tccttagagg ccgcctggat cagttgctca ggcacgtgat gaaccccttg gaaaccctct
1141 caataactaa ctgccggctt tcggaagggg atgtgatgca tctgtcccag agtcccagcg
1201 tcagtcagct aagtgtcctg agtctaagtg gggtcatgct gaccgatgta agtcccgagc
1261 ccctccaagc tctgctggag agagcctctg ccaccctcca ggacctggtc tttgatgagt
1321 gtgggatcac ggatgatcag ctccttgccc tcctgccttc cctgagccac tgctcccagc
1381 ttacaacctt aagcttctac gggaattcca tctccatatc tgccttgcag agtctcctgc
1441 agcacctcat cgggctgagc aatctgaccc acgtgctgta tcctgtcccc ctggagagtt
1501 atgaggacat ccatggtacc ctccacctgg agaggcttgc ctatctgcat gccaggctca
1561 gggagttgct gtgtgagttg gggcggccca gcatggtctg gcttagtgcc aacccctgtc
1621 ctcactgtgg ggacagaacc ttctatgacc cggagcccat cctgtgcccc tgtttcatgc
1681 ctaactagct gggtgcacat atcaaatgct tcattctgca tacttggaca ctaaagccag
1741 gatgtgcatg catcttgaag caacaaagca gccacagttt cagacaaatg ttcagtgtga
1801 gtgaggaaaa catgttcagt gaggaaaaaa cattcagaca aatgttcagt gaggaaaaaa
1861 aggggaagtt ggggataggc agatgttgac ttgaggagtt aatgtgatct ttggggagat
1921 acatcttata gagttagaaa tagaatctga atttctaaag ggagattctg gcttgggaag
1981 tacatgtagg agttaatccc tgtgtagact gttgtaaaga aactgttgaa aataaagaga
2041 agcaatgtga aaaaaaaaaa aaaaaaa
//