LOCUS BC014039 2501 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens maternal embryonic leucine zipper kinase, mRNA (cDNA
clone MGC:20350 IMAGE:4547136), complete cds.
ACCESSION BC014039
VERSION BC014039.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2501)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2501)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC014039.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 28 Row: p Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 41281490.
FEATURES Location/Qualifiers
source 1..2501
/db_xref="H-InvDB:HIT000036484"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20350 IMAGE:4547136"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_15"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2501
/gene="MELK"
/gene_synonym="HPK38"
/gene_synonym="KIAA0175"
/db_xref="GeneID:9833"
/db_xref="HGNC:HGNC:16870"
/db_xref="MIM:607025"
CDS 139..2094
/gene="MELK"
/gene_synonym="HPK38"
/gene_synonym="KIAA0175"
/codon_start=1
/product="maternal embryonic leucine zipper kinase"
/protein_id="AAH14039.1"
/db_xref="GeneID:9833"
/db_xref="HGNC:HGNC:16870"
/db_xref="MIM:607025"
/translation="MKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDK
NTLGSDLPRIKTEIEALKNLRHQHICQLYHVLETANKIFMVLEYCPGGELFDYIISQD
RLSEEETRVVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLIDFGLCAKPKGN
KDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALY
KKIMRGKYDVPKWLSPSSILLLQQMLQVDPKKRISMKNLLNHPWIMQDYNYPVEWQSK
NPFIHLDDDCVTELSVHHRNNRQTMEDLISLWQYDHLTATYLLLLAKKARGKPVRLRL
SSFSCGQASATPFTDIKSNNWSLEDVTASDKNYVAGLIDYDWCEDDLSTGAATPRTSQ
FTKYWTESNGVESKSLTPALCRTPANKLKNKENVYTPKSAVKNEEYFMFPEPKTPVNK
NQHKREILTTPNRYTTPSKARNQCLKETPIKIPVNSTGTDKLMTGVISPERRCRSVEL
DLNQAHMEETPKRKGAKVFGSLERGLDKVITVLTRSKRKGSARDGPRRLKLHYNVTTT
RLVNPDQLLNEIMSILPKKHVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQKPDVV
GIRRQRLKGDAWVYKRLVEDILSSCKV"
BASE COUNT 824 a 478 c 523 g 676 t
ORIGIN
1 cgaaaagatt cttaggaacg ccgtaccagc cgcgtctctc aggacagcag gcccctgtcc
61 ttctgtcggg cgccgctcag ccgtgccctc cgcccctcag gttctttttc taattccaaa
121 taaacttgca agaggactat gaaagattat gatgaacttc tcaaatatta tgaattacat
181 gaaactattg ggacaggtgg ctttgcaaag gtcaaacttg cctgccatat ccttactgga
241 gagatggtag ctataaaaat catggataaa aacacactag ggagtgattt gccccggatc
301 aaaacggaga ttgaggcctt gaagaacctg agacatcagc atatatgtca actctaccat
361 gtgctagaga cagccaacaa aatattcatg gttcttgagt actgccctgg aggagagctg
421 tttgactata taatttccca ggatcgcctg tcagaagagg agacccgggt tgtcttccgt
481 cagatagtat ctgctgttgc ttatgtgcac agccagggct atgctcacag ggacctcaag
541 ccagaaaatt tgctgtttga tgaatatcat aaattaaagc tgattgactt tggtctctgt
601 gcaaaaccca agggtaacaa ggattaccat ctacagacat gctgtgggag tctggcttat
661 gcagcacctg agttaataca aggcaaatca tatcttggat cagaggcaga tgtttggagc
721 atgggcatac tgttatatgt tcttatgtgt ggatttctac catttgatga tgataatgta
781 atggctttat acaagaagat tatgagagga aaatatgatg ttcccaagtg gctctctccc
841 agtagcattc tgcttcttca acaaatgctg caggtggacc caaagaaacg gatttctatg
901 aaaaatctat tgaaccatcc ctggatcatg caagattaca actatcctgt tgagtggcaa
961 agcaagaatc cttttattca cctcgatgat gattgcgtaa cagaactttc tgtacatcac
1021 agaaacaaca ggcaaacaat ggaggattta atttcactgt ggcagtatga tcacctcacg
1081 gctacctatc ttctgcttct agccaagaag gctcggggaa aaccagttcg tttaaggctt
1141 tcttctttct cctgtggaca agccagtgct accccattca cagacatcaa gtcaaataat
1201 tggagtctgg aagatgtgac cgcaagtgat aaaaattatg tggcgggatt aatagactat
1261 gattggtgtg aagatgattt atcaacaggt gctgctactc cccgaacatc acagtttacc
1321 aagtactgga cagaatcaaa tggggtggaa tctaaatcat taactccagc cttatgcaga
1381 acacctgcaa ataaattaaa gaacaaagaa aatgtatata ctcctaagtc tgctgtaaag
1441 aatgaagagt actttatgtt tcctgagcca aagactccag ttaataagaa ccagcataag
1501 agagaaatac tcactacgcc aaatcgttac actacaccct caaaagctag aaaccagtgc
1561 ctgaaagaaa ctccaattaa aataccagta aattcaacag gaacagacaa gttaatgaca
1621 ggtgtcatta gccctgagag gcggtgccgc tcagtggaat tggatctcaa ccaagcacat
1681 atggaggaga ctccaaaaag aaagggagcc aaagtgtttg ggagccttga aagggggttg
1741 gataaggtta tcactgtgct caccaggagc aaaaggaagg gttctgccag agacgggccc
1801 agaagactaa agcttcacta taatgtgact acaactagat tagtgaatcc agatcaactg
1861 ttgaatgaaa taatgtctat tcttccaaag aagcatgttg actttgtaca aaagggttat
1921 acactgaagt gtcaaacaca gtcagatttt gggaaagtga caatgcaatt tgaattagaa
1981 gtgtgccagc ttcaaaaacc cgatgtggtg ggtatcagga ggcagcggct taagggcgat
2041 gcctgggttt acaaaagatt agtggaagac atcctatcta gctgcaaggt ataattgatg
2101 gattcttcca tcctgccgga tgagtgtggg tgtgatacag cctacataaa gactgttatg
2161 atcgctttga ttttaaagtt cattggaact accaacttgt ttctaaagag ctatcttaag
2221 accaatatct ctttgttttt aaacaaaaga tattattttg tgtatgaatc taaatcaagc
2281 ccatctgtca ttatgttact gtctttttta atcatgtggt tttgtatatt aataattgtt
2341 gactttctta gattcacttc catatgtgaa tgtaagctct taactatgtc tctttgtaat
2401 gtgtaatttc tttctgaaat aaaaccattt gtgaatataa aaaaaaaaaa aaaaaaaaaa
2461 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//