LOCUS BC013579 2680 bp mRNA linear SYN 02-SEP-2016
DEFINITION Synthetic construct Homo sapiens calpastatin, mRNA (cDNA clone
MGC:9402 IMAGE:3878564), complete cds.
ACCESSION BC013579
VERSION BC013579.1
KEYWORDS MGC.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 2680)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2680)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (04-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP/Gazdar
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 14 Row: c Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 27765082.
FEATURES Location/Qualifiers
source 1..2680
/db_xref="H-InvDB:HIT000036286"
/organism="synthetic construct"
/mol_type="mRNA"
/isolation_source="Homo sapiens; Lung, large cell
carcinoma"
/db_xref="taxon:32630"
/clone="MGC:9402 IMAGE:3878564"
/clone_lib="NIH_MGC_68"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2680
/gene="CAST"
/gene_synonym="BS-17"
/gene_synonym="MGC9402"
/db_xref="GeneID:831"
/db_xref="MIM:114090"
CDS 219..2222
/gene="CAST"
/gene_synonym="BS-17"
/gene_synonym="MGC9402"
/codon_start=1
/transl_table=11
/product="CAST protein"
/protein_id="AAH13579.1"
/db_xref="GeneID:831"
/db_xref="MIM:114090"
/translation="MNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVS
RSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESK
PDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTI
PPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEV
LKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDE
AKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSED
FDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDA
VEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDG
KPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPAS
TTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKL
GERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTT
ETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD"
misc_feature 375..767
/gene="CAST"
/gene_synonym="BS-17"
/gene_synonym="MGC9402"
/note="Calpain_inhib; Region: Calpain inhibitor. This
region is found multiple times in calpain inhibitor
proteins"
/db_xref="CDD:pfam00748"
misc_feature 774..1142
/gene="CAST"
/gene_synonym="BS-17"
/gene_synonym="MGC9402"
/note="Calpain_inhib; Region: Calpain inhibitor. This
region is found multiple times in calpain inhibitor
proteins"
/db_xref="CDD:pfam00748"
misc_feature 1302..1598
/gene="CAST"
/gene_synonym="BS-17"
/gene_synonym="MGC9402"
/note="Calpain_inhib; Region: Calpain inhibitor. This
region is found multiple times in calpain inhibitor
proteins"
/db_xref="CDD:pfam00748"
misc_feature 1614..2009
/gene="CAST"
/gene_synonym="BS-17"
/gene_synonym="MGC9402"
/note="Calpain_inhib; Region: Calpain inhibitor. This
region is found multiple times in calpain inhibitor
proteins"
/db_xref="CDD:pfam00748"
BASE COUNT 948 a 566 c 602 g 564 t
ORIGIN
1 ctagttctag atcgcgagcg gccgccgccc gccgcaccca tgagcatgtc agtgaaaaaa
61 ccagtgaatc gccttccaaa ccaggagaaa agaaaggatc agatgagaaa aaagcagcaa
121 gcctcggcag cagtcaatcc tccagaacct atgctggtgg aacagcctcg gccaccaagg
181 tgtcagcttc ctctggtgca accagcaagt cttccagtat gaatcccaca gaaaccaagg
241 ctgtaaaaac agaacctgag aagaagtcac agtcaaccaa gccaaaaagc ctacccaagc
301 aggcatcaga tacaggaagt aacgatgctc acaataaaaa agcagtttcc agatcagctg
361 aacagcagcc atcagagaaa tcaacagaac caaagactaa accacaagac atgatttctg
421 ctggtggaga gagtgttgct ggtatcactg caatatctgg caagccgggt gacaagaaaa
481 aagaaaagaa atcattaacc ccagctgtgc cagttgaatc taaaccggat aaaccatcgg
541 gaaagtcagg catggatgct gctttggatg acttaataga tactttagga ggacctgaag
601 aaactgaaga agaaaataca acgtatactg gaccagaagt ttcagatcca atgagttcca
661 cctacataga ggaattgggt aaaagagaag tcacaattcc tccaaaatat agggaactat
721 tggctaaaaa ggaagggatc acagggcctc ctgcagactc ttcgaaaccc atagggccag
781 atgatgctat agacgccttg tcatctgact tcacctgtgg gtcgcctaca gctgctggaa
841 agaaaactga aaaagaggaa tctacagaag ttttaaaagc tcagtcagca gggacagtca
901 gaagtgctgc tccaccccaa gagaagaaaa gaaaggtgga gaaggataca atgagtgatc
961 aagcactcga ggctctgtcg gcttcactgg gcacccggca agcagaacct gagctcgacc
1021 tccgctcaat taaggaagtc gatgaggcaa aagctaaaga agaaaaacta gagaagtgtg
1081 gtgaggatga tgaaacaatc ccatctgagt acagattaaa accagccacg gataaagatg
1141 gaaaaccact attgccagag cctgaagaaa aacccaagcc tcggagtgaa tcagaactca
1201 ttgatgaact ttcagaagat tttgaccggt ctgaatgtaa agagaaacca tctaagccaa
1261 ctgaaaagac agaagaatct aaggccgctg ctccagctcc tgtgtcggag gctgtgtgtc
1321 ggacctccat gtgtagtata cagtcagcac cccctgagcc ggctaccttg aagggcacag
1381 tgccagatga tgctgtagaa gccttggctg atagcctggg gaaaaaggaa gcagatccag
1441 aagatggaaa acctgtgatg gataaagtca aggagaaggc caaagaagaa gaccgtgaaa
1501 agcttggtga aaaagaagaa acaattcctc ctgattatag attagaagag gtcaaggata
1561 aagatggaaa gccactcctg ccaaaagagt ctaaggaaca gcttccaccc atgagtgaag
1621 acttccttct ggatgctttg tctgaggact tctctggtcc acaaaatgct tcatctctta
1681 aatttgaaga tgctaaactt gctgctgcca tctctgaagt ggtttcccaa accccagctt
1741 caacgaccca agctggagcc ccaccccgtg atacctcgca gagtgacaaa gacctcgatg
1801 atgccttgga taaactctct gacagtctag gacaaaggca gcctgaccca gatgagaaca
1861 aaccaatgga agataaagta aaggaaaaag ctaaagctga acatagagac aagcttggag
1921 aaagagatga cactatccca cctgaataca gacatctcct ggatgataat ggacaggaca
1981 aaccagtgaa gccacctaca aagaaatcag aggattcaaa gaaacctgca gatgaccaag
2041 accccattga tgctctctca ggagatctgg acagctgtcc ctccactaca gaaacctcac
2101 agaacacagc aaaggataag tgcaagaagg ctgcttccag ctccaaagca cctaagaatg
2161 gaggtaaagc gaaggattca gcaaagacaa cagaggaaac ttccaagcca aaagatgact
2221 aaagaaatac aagttaaggt atctggtatc tgcatgtaaa atcttcagct ggtggatggt
2281 gacttttgaa gaacaaaagg ctttggcaac agaaaacaat tgttctgggt gatttctaga
2341 atggtttttg ttgagtctct gaacatccta aatattggtt tgttattctt ttccagaaag
2401 aaaatgaatt tgactggttc acctgtgtac tgagtattga taaactttga atttttttaa
2461 ttgccttcaa ttgggagaga aagctttata tttgtaagaa atatatttga taaagtttct
2521 taaagcaaca ccaaaaaaac aaaagaaaag ctaagtgaat ttttgcacat tctacacaca
2581 gtgcctgtaa atctcatttg tattttcagt ttgcccttaa ttttttttgt tagtgtttag
2641 aaaacaatgt tttaaacatt aaaaaaaaaa aaaaaaaaaa
//