LOCUS BC013314 2638 bp mRNA linear HUM 11-DEC-2003
DEFINITION Homo sapiens N-ethylmaleimide-sensitive factor, mRNA (cDNA clone
IMAGE:3939600), partial cds.
ACCESSION BC013314
VERSION BC013314.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2638)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2638)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 9, 2003 this sequence version replaced BC013314.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 14 Row: f Column: 24.
FEATURES Location/Qualifiers
source 1..2638
/db_xref="H-InvDB:HIT000088090"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3939600"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..2638
/gene="NSF"
/gene_synonym="SKD2"
/db_xref="GeneID:4905"
/db_xref="MIM:601633"
CDS <1..982
/gene="NSF"
/gene_synonym="SKD2"
/codon_start=2
/product="NSF protein"
/protein_id="AAH13314.2"
/db_xref="GeneID:4905"
/db_xref="MIM:601633"
/translation="LLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTK
VEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDD
GELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSE
TAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQ
GRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTI
AQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALLREEGASPLDFD"
misc_feature 359..571
/gene="NSF"
/gene_synonym="SKD2"
/note="AAA; Region: ATPase family associated with various
cellular activities (AAA). AAA family proteins often
perform chaperone-like functions that assist in the
assembly, operation, or disassembly of protein complexes"
/db_xref="CDD:pfam00004"
BASE COUNT 752 a 558 c 558 g 770 t
ORIGIN
1 gttactctct gctgatgtag acattaaaga actggccgtg gagaccaaga atttcagtgg
61 tgctgaattg gagggtctgg tgcgagcagc ccagtccact gctatgaata gacacataaa
121 ggccagtact aaagtggaag tggacatgga gaaagcagaa agcctgcaag tgacgagagg
181 agacttcctt gcttctttgg agaatgatat caaaccagcc tttggcacaa accaagaaga
241 ttatgcaagt tacattatga acggtatcat caaatggggt gacccagtta ctcgagttct
301 agatgatggg gagctgctgg tgcagcagac taagaacagt gaccgcacac cattggtcag
361 cgtgcttctg gaaggccctc ctcacagtgg gaagactgct ttagctgcaa aaattgcaga
421 ggaatccaac ttcccgttca tcaagatctg ttctcctgat aaaatgattg gcttttccga
481 aacagccaaa tgtcaggcca tgaagaagat ctttgatgat gcgtacaaat cccagctcag
541 ttgtgtggtt gtggatgaca ttgagagatt gcttgattac gtccctattg gccctcgatt
601 ttcaaatctt gtattacagg ctcttctcgt tttactgaaa aaggcacctc ctcagggccg
661 caagcttctt atcattggga ccactagccg caaagatgtc cttcaggaga tggaaatgct
721 taacgctttc agcaccacca tccacgtgcc caacattgcc acaggagagc agctgttgga
781 agctttggag cttttgggca acttcaagga taaggaacgc accacaattg cacagcaagt
841 caaagggaag aaagtctgga taggaatcaa gaagttacta atgctgatcg agatgtccct
901 acagatggat cctgaatacc gtgtgagaaa attcttggcc ctcttaagag aagaaggagc
961 tagccccctt gattttgatt gaaaatgaac tatttgaaac acacagtgac caagggaagt
1021 gaccaaggtg aagatggcct aggatcttca ctgtcttact caagatactg gactaagtgg
1081 aacgttctct accttcaaca tgtgctcgct ctgcatgatt agtgcaataa aactcccttc
1141 cttatgcata ctgagatagc ttagtgtctc gtggaaggtg tcaatttggt ttagaatgct
1201 gcgcttacct tcccatgcag gctaaagtga ttccttcttg ctcagtccct ctgggtggga
1261 accatccagt acttgtggac actacacgtt tcaacctctc tactagcacc atcacccttg
1321 aaaactctca gtcagtgtca tgaatgttgc atgacaacag ttggccgatt agaaggcaga
1381 ctttctacat gcaaatctgg cttagtaaat cgaggtgtgg gccagagatc ctctgacagc
1441 tgtcctgagc taacactaaa agtcactggg tatttggtta aaggtctccc acaagactgg
1501 tattctcttt gcctgaagaa acaaggcatt gaatctctaa aatgctgttc tcaatcattg
1561 tcagagatgt tttcaagttg cagtcagaag atctttctta atagaaagtc agatgactac
1621 cgtgttggtt gtgacttccc cttaagtata actaatttgc tctgtggtaa gagatatgct
1681 cattattacc acttagaaga tgttgttaaa aacatgtgaa agataggtat ggaaaaagca
1741 tacaccccca aacagaaagg agttattaaa gtaatttaca aacctctcag cactaattag
1801 tgtccaactc caagtgggtc aattccttag tataatatta aggcttacta gtatcactgc
1861 tttttcctta gcttaatgac ttacttagaa tttatccttt attttaaatg atctgtacta
1921 tctagtgtct aaaacactat tctccagaaa aatcaatcat tttctagccc tctccctcag
1981 tcctttattg tccattccaa tacattgaac acatttcctt taccctccac acacttcttc
2041 caaaaggaag cacccgttga gtccttttga gggtgatttg tcttacaact gactgactta
2101 gcaggaattt aattaggtca tatttggtga tgagacttat ggagtgtgcc tctctctccc
2161 aactgctgct taaaatgcaa ggacaagcaa ttagaagcca tcctaaggtg cttacctcac
2221 acgccaccca tgaggcttgt ggccacagtg gcacttgggt gtggctcctc tgttatttgt
2281 cctcatgtga gaaagcagat catctccaaa tcttgccatt tgtatacttt tggtggagac
2341 ttggatgtca tatcttcttt gttttgggtt ttcttcccta gcttattttg tggcttttaa
2401 agaagtggat tgtattgtga gatcctgtga ttcctggtgg ccagtatcct ggattcctct
2461 aagatcttgc ctctttcctc ctcatgaaag cagcacacat tgtgttaact tatgtctctt
2521 gttaaatgag cttaatgtct ttgtgttttg tccaaaactg tattgaaaaa atattgttta
2581 atgcaaatga aggaatgcaa taaagagtaa atatacttga aaaaaaaaaa aaaaaaaa
//