LOCUS BC013023 4313 bp mRNA linear HUM 12-NOV-2003
DEFINITION Homo sapiens nuclear mitotic apparatus protein 1, mRNA (cDNA clone
MGC:4620 IMAGE:3506457), complete cds.
ACCESSION BC013023
VERSION BC013023.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4313)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4313)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (20-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC013023.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 11 Row: e Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..4313
/db_xref="H-InvDB:HIT000050571"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:4620 IMAGE:3506457"
/tissue_type="Kidney, renal cell adenocarcinoma"
/clone_lib="NIH_MGC_14"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..4313
/gene="NUMA1"
/gene_synonym="NUMA"
/db_xref="GeneID:4926"
/db_xref="MIM:164009"
CDS 7..3588
/gene="NUMA1"
/gene_synonym="NUMA"
/codon_start=1
/product="NUMA1 protein"
/protein_id="AAH13023.1"
/db_xref="GeneID:4926"
/db_xref="MIM:164009"
/translation="MAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWL
EEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLT
QERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAK
LRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALR
AEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSA
LASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVL
EKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREE
AEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVS
ELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGALRAELLRAQRELGELIPLRQKV
AEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQARE
KYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQK
LTAQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKE
QAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQA
GLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDL
SIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPI
PARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFY
STRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSG
APPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTIT
DEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCF
PRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGA
SKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH"
BASE COUNT 1049 a 1237 c 1371 g 656 t
ORIGIN
1 gaaaagatgg ctgccaccag caaagaggtg gcccgcttgg agaccttggt gcgcaaggca
61 ggtgagcagc aggaaacagc ctcccgggag ttagtcaagg agcctgcgag ggcaggagac
121 agacagcccg agtggctgga agagcaacag ggacgccagt tctgcagcac acaggcagcg
181 ctgcaggcta tggagcggga ggcagagcag atgggcaatg agctggaacg gctgcgggcc
241 gcgctgatgg agagccaggg gcagcagcag gaggagcgtg ggcagcagga aagggaggtg
301 gcgcggctga cccaggagcg gggccgtgcc caggctgacc ttgccctgga gaaggcggcc
361 agagcagagc ttgagatgcg gctgcagaac gccctcaacg agcagcgtgt ggagttcgct
421 accctgcaag aggcactggc tcatgccctg acggaaaagg aaggcaagga ccaggagttg
481 gccaagcttc gtggtctgga ggcagcccag ataaaagagc tggaggaact tcggcaaacc
541 gtgaagcaac tgaaggaaca gctggctaag aaagaaaagg agcacgcatc tggctcagga
601 gcccaatctg aggctgctgg caggacagag ccaacaggcc ccaagctgga ggcactgcgg
661 gcagaggtga gcaagctgga acagcaatgc cagaagcagc aggagcaggc tgacagcctg
721 gaacgcagcc tcgaggctga gcgggcctcc cgggctgagc gggacagtgc tctggagact
781 ctgcagggcc agttagagga gaaggcccag gagctagggc acagtcagag tgccttagcc
841 tcggcccaac gggagttggc tgccttccgc accaaggtac aagaccacag caaggctgaa
901 gatgagtgga aggcccaggt ggcccggggc cggcaagagg ctgagaggaa aaatagcctc
961 atcagcagct tggaggagga ggtgtccatc ctgaatcgcc aggtcctgga gaaggagggg
1021 gagagcaagg agttgaagcg gctggtgatg gccgagtcag agaagagcca gaagctggag
1081 gagaggctgc gcctgctgca ggcagagaca gccagcaaca gtgccagagc tgcagaacgc
1141 agctctgctc tgcgggagga ggtgcagagc ctccgggagg aggctgagaa acagcgggtg
1201 gcttcagaga acctgcggca ggagctgacc tcacaggctg agcgtgcgga ggagctgggc
1261 caagaattga aggcgtggca ggagaagttc ttccagaaag agcaggccct ctccaccctg
1321 cagctcgagc acaccagcac acaggccctg gtgagtgagc tgctgccagc taagcacctc
1381 tgccagcagc tgcaggccga gcaggccgct gccgagaaac gccaccgtga ggagctggag
1441 cagagcaagc aggccgctgg ggcactgcgg gcagagctgc tgcgggccca gcgggagctt
1501 ggggagctga ttcctctgcg gcagaaggtg gcagagcagg agcgaacagc tcagcagctg
1561 cgggcagaga aggccagcta tgcagagcag ctgagcatgc tgaagaaggc gcatggcctg
1621 ctggcagagg agaaccgggg gctgggtgag cgggccaacc ttggccggca gtttctggaa
1681 gtggagttgg accaggcccg ggagaagtat gtccaagagt tggcagccgt acgtgctgat
1741 gctgagaccc gtctggctga ggtgcagcga gaagcacaga gcactgcccg ggagctggag
1801 gtgatgactg ccaagtatga gggtgccaag gtcaaggtcc tggaggagag gcagcggttc
1861 caggaagaga ggcagaaact cactgcccag gtggaagaac tgagtaagaa actggctgac
1921 tctgaccaag ccagcaaggt gcagcagcag aagctgaagg ctgtccaggc tcagggaggc
1981 gagagccagc aggaggccca gcgcctccag gcccagctga atgaactgca agcccagttg
2041 agccagaagg agcaggcagc tgagcactat aagctgcaga tggagaaagc caaaacacat
2101 tatgatgcca agaagcagca gaaccaagag ctgcaggagc agctgcggag cctggagcag
2161 ctgcagaagg aaaacaaaga gctgcgagct gaagctgaac ggctgggcca tgagctacag
2221 caggctgggc tgaagaccaa ggaggctgaa cagacctgcc gccaccttac tgcccaggtg
2281 cgcagcctgg aggcacaggt tgcccatgca gaccagcagc ttcgagacct gggcaaattc
2341 caggtggcaa ctgatgcttt aaagagccgt gagccccagg ctaagcccca gctggacttg
2401 agtattgaca gcctggatct gagctgcgag gaggggaccc cactcagtat caccagcaag
2461 ctgcctcgta cccagccaga cggcaccagc gtccctggag aaccagcctc acctatctcc
2521 cagcgcctgc cccccaaggt agaatccctg gagagtctct acttcactcc catccctgct
2581 cggagtcagg cccccctgga gagcagcctg gactccctgg gagacgtctt cctggactcg
2641 ggtcgtaaga cccgctccgc tcgtcggcgc accacgcaga tcatcaacat caccatgacc
2701 aagaagctag atgtggaaga gccagacagc gccaactcat cgttctacag cacgcggtct
2761 gctcctgctt cccaggctag cctgcgagcc acctcctcta ctcagtctct agctcgcctg
2821 ggttctcccg attatggcaa ctcagccctg ctcagcttgc ctggctaccg ccccaccact
2881 cgcagttctg ctcgtcgttc ccaggccggg gtgtccagtg gggcccctcc aggaaggaac
2941 agcttctaca tgggcacttg ccaggatgag cctgagcagc tggatgactg gaaccgcatt
3001 gcagagctgc agcagcgcaa tcgagtgtgc cccccacatc tgaagacctg ctatcccctg
3061 gagtccaggc cttccctgag cctgggcacc atcacagatg aggagatgaa aactggagac
3121 ccccaagaga ccctgcgccg agccagcatg cagccaatcc agatagccga gggcactggc
3181 atcaccaccc ggcagcagcg caaacgggtc tccctagagc cccaccaggg ccctggaact
3241 cctgagtcta agaaggccac cagctgtttc ccacgcccca tgactccccg agaccgacat
3301 gaagggcgca aacagagcac tactgaggcc cagaagaaag cagctccagc ttctactaaa
3361 caggctgacc ggcgccagtc gatggccttc agcatcctca acacacccaa gaagctaggg
3421 aacagccttc tgcggcgggg agcctcaaag aaggccctgt ccaaggcttc ccccaacact
3481 cgcagtggaa cccgccgttc tccgcgcatt gccaccacca cagccagcgc cgccactgct
3541 gccgccattg gtgccacccc tcgagccaag ggcaaggcaa agcactaaag ggccagtacc
3601 agtgagtggc cccacctgtg tccccgatgc tgccgtcacc tggtcctccg cctactgtcc
3661 ctctcagtgc cttctctcag ctcccaggcc aacagtagcc aaacccctag agacagtgat
3721 gcctgcccgc accctggcct ggtccctggt ccttcactgg cgccttctcg gagctggccc
3781 agggggcctg gagcatggac agtgtgggcg ctctccctac cttgcctcct tttttcttaa
3841 agcaaagtca cttctccatc acaaccagat ttgaggctgg ttttgatggc tgggtccttg
3901 ggcctggcca gtcttcctct tagcctctgg atctagaagg gaccataaga ggagtaggcc
3961 ctggttcctg ctgtcctggt ggctgggccc agcaggggcc ctcactcttg aagtccagga
4021 ctgggtctga cctggtggga gcacctgcca gaggatgctc tttcccagga cggatgggcc
4081 ctgtgtctca ggagtggggt tgggggacag ccttcagcag cagctcacac cctaccttcc
4141 ccagacttgc actggggtgg gatttggagt gatgggaagg tttttaaggg ccggggatgg
4201 atcttttcta aatgttatta cttgtaaata aagtctattt ttctcccgtg aaaaaaaaaa
4261 aaaaaaccaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
//