LOCUS BC012999 2977 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens kinesin family member 20A, mRNA (cDNA clone MGC:3571
IMAGE:3604291), complete cds.
ACCESSION BC012999
VERSION BC012999.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2977)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2977)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (20-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Oct 31, 2003 this sequence version replaced BC012999.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 11 Row: l Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 5032012.
FEATURES Location/Qualifiers
source 1..2977
/db_xref="H-InvDB:HIT000036070"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3571 IMAGE:3604291"
/tissue_type="Uterus, endometrium adenocarcinoma"
/clone_lib="NIH_MGC_44"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2977
/gene="KIF20A"
/gene_synonym="MKLP2"
/db_xref="GeneID:10112"
/db_xref="HGNC:HGNC:9787"
/db_xref="MIM:605664"
CDS 35..2707
/gene="KIF20A"
/gene_synonym="MKLP2"
/codon_start=1
/product="kinesin family member 20A"
/protein_id="AAH12999.1"
/db_xref="GeneID:10112"
/db_xref="HGNC:HGNC:9787"
/db_xref="MIM:605664"
/translation="MSQGILSPPAGLLSDDDVVVSPMFESTAADLGSVVRKNLLSDCS
VVSTSLEDKQQVPSEDSMEKVKVYLRVRPLLPSELERQEDQGCVRIENVETLVLQAPK
DSFALKSNERGIGQATHRFTFSQIFGPEVGQASFFNLTVKEMVKDVLKGQNWLIYTYG
VTNSGKTHTIQGTIKDGGILPRSLALIFNSLQGQLHPTPDLKPLLSNEVIWLDSKQIR
QEEMKKLSLLNGGLQEEELSTSLKRSVYIESRIGTSTSFDSGIAGLSSISQCTSSSQL
DETSHRWAQPDTAPLPVPANIRFSIWISFFEIYNELLYDLLEPPSQQRKRQTLRLCED
QNGNPYVKDLNWIHVQDAEEAWKLLKVGRKNQSFASTHLNQNSSRSHSIFSIRILHLQ
GEGDIVPKISELSLCDLAGSERCKDQKSGERLKEAGNINTSLHTLGRCIAALRQNQQN
RSKQNLVPFRDSKLTRVFQGFFTGRGRSCMIVNVNPCASTYDETLHVAKFSAIASQLV
HAPPMQLGFPSLHSFIKEHSLQVSPSLEKGAKADTGLDDDIENEADISMYGKEELLQV
VEAMKTLLLKERQEKLQLEMHLRDEICNEMVEQMQQREQWCSEHLDTQKELLEEMYEE
KLNILKESLTSFYQEEIQERDEKIEELEALLQEARQQSVAHQQSGSELALRRSQRLAA
SASTQQLQEVKAKLQQCKAELNSTTEELHKYQKMLEPPPSAKPFTIDVDKKLEEGQKN
IRLLRTELQKLGESLQSAERACCHSTGAGKLRQALTTCDDILIKQDQTLAELQNNMVL
VKLDLRKKAACIAEQYHTVLKLQGQVSAKKRLGTNQENQQPNQQPPGKKPFLRNLLPR
TPTCQSSTDCSPYARILRSRRSPLLKSGPFGKKY"
BASE COUNT 859 a 731 c 704 g 683 t
ORIGIN
1 ggcacgtctt cggacctagg ctgcccctgc cgtcatgtcg caagggatcc tttctccgcc
61 agcgggcttg ctgtccgatg acgatgtcgt agtttctccc atgtttgagt ccacagctgc
121 agatttgggg tctgtggtac gcaagaacct gctatcagac tgctctgtcg tctctacctc
181 cctagaggac aagcagcagg ttccatctga ggacagtatg gagaaggtga aagtatactt
241 gagggttagg cccttgttac cttcagagtt ggaacgacag gaagatcagg gttgtgtccg
301 tattgagaat gtggagaccc ttgttctaca agcacccaag gactcttttg ccctgaagag
361 caatgaacgg ggaattggcc aagccacaca caggttcacc ttttcccaga tctttgggcc
421 agaagtggga caggcatcct tcttcaacct aactgtgaag gagatggtaa aggatgtact
481 caaagggcag aactggctca tctatacata tggagtcact aactcaggga aaacccacac
541 gattcaaggt accatcaagg atggagggat tctcccccgg tccctggcgc tgatcttcaa
601 tagcctccaa ggccaacttc atccaacacc tgatctgaag cccttgctct ccaatgaggt
661 aatctggcta gacagcaagc agatccgaca ggaggaaatg aagaagctgt ccctgctaaa
721 tggaggcctc caagaggagg agctgtccac ttccttgaag aggagtgtct acatcgaaag
781 tcggataggt accagcacca gcttcgacag tggcattgct gggctctctt ctatcagtca
841 gtgtaccagc agtagccagc tggatgaaac aagtcatcga tgggcacagc cagacactgc
901 cccactacct gtcccggcaa acattcgctt ctccatctgg atctcattct ttgagatcta
961 caacgaactg ctttatgacc tattagaacc gcctagccaa cagcgcaaga ggcagacttt
1021 gcggctatgc gaggatcaaa atggcaatcc ctatgtgaaa gatctcaact ggattcatgt
1081 gcaagatgct gaggaggcct ggaagctcct aaaagtgggt cgtaagaacc agagctttgc
1141 cagcacccac ctcaaccaga actccagccg cagtcacagc atcttctcaa tcaggatcct
1201 acaccttcag ggggaaggag atatagtccc caagatcagc gagctgtcac tctgtgatct
1261 ggctggctca gagcgctgca aagatcagaa gagtggtgaa cggttgaagg aagcaggaaa
1321 cattaacacc tctctacaca ccctgggccg ctgtattgct gcccttcgtc aaaaccagca
1381 gaaccggtca aagcagaacc tggttccctt ccgtgacagc aagttgactc gagtgttcca
1441 aggtttcttc acaggccgag gccgttcctg catgattgtc aatgtgaatc cctgtgcatc
1501 tacctatgat gaaactcttc atgtggccaa gttctcagcc attgctagcc agcttgtgca
1561 tgccccacct atgcaactgg gattcccatc cctgcactcg ttcatcaagg aacatagtct
1621 tcaggtatcc cccagcttag agaaaggggc taaggcagac acaggccttg atgatgatat
1681 tgaaaatgaa gctgacatct ccatgtatgg caaagaggag ctcctacaag ttgtggaagc
1741 catgaagaca ctgcttttga aggaacgaca ggaaaagcta cagctggaga tgcatctccg
1801 agatgaaatt tgcaatgaga tggtagaaca gatgcaacag cgggaacagt ggtgcagtga
1861 acatttggac acccaaaagg aactattgga ggaaatgtat gaagaaaaac taaatatcct
1921 caaggagtca ctgacaagtt tttaccaaga agagattcag gagcgggatg aaaagattga
1981 agagctagaa gctctcttgc aggaagccag acaacagtca gtggcccatc agcaatcagg
2041 gtctgaattg gccctacggc ggtcacaaag gttggcagct tctgcctcca cccagcagct
2101 tcaggaggtt aaagctaaat tacagcagtg caaagcagag ctaaactcta ccactgaaga
2161 gttgcataag tatcagaaaa tgttagaacc accaccctca gccaagccct tcaccattga
2221 tgtggacaag aagttagaag agggccagaa gaatataagg ctgttgcgga cagagcttca
2281 gaaacttggt gagtctctcc aatcagcaga gagagcttgt tgccacagca ctggggcagg
2341 aaaacttcgt caagccttga ccacttgtga tgacatctta atcaaacagg accagactct
2401 ggctgaactg cagaacaaca tggtgctagt gaaactggac cttcggaaga aggcagcatg
2461 tattgctgag cagtatcata ctgtgttgaa actccaaggc caggtttctg ccaaaaagcg
2521 ccttggtacc aaccaggaaa atcagcaacc aaaccaacaa ccaccaggga agaaaccatt
2581 ccttcgaaat ttacttcccc gaacaccaac ctgccaaagc tcaacagact gcagccctta
2641 tgcccggatc ctacgctcac ggcgttcccc tttactcaaa tctgggcctt ttggcaaaaa
2701 gtactaaggc tgtggggaaa gagaagagca gtcatggccc tgaggtgggt cagctactct
2761 cctgaagaaa taggtctctt ttatgcttta ccatatatca ggaattatat ccaggatgca
2821 atactcagac actagctttt ttctcacttt tgtattataa ccacctatgt aatctcatgt
2881 tgttgttttt ttttatttac ttatatgatt tctatgcaca caaaaacagt tatattaaag
2941 atattattgt tcacatttta aaaaaaaaaa aaaaaaa
//