LOCUS BC012946 2151 bp mRNA linear HUM 01-SEP-2006
DEFINITION Homo sapiens Rho-guanine nucleotide exchange factor, mRNA (cDNA
clone IMAGE:4454960), complete cds.
ACCESSION BC012946
VERSION BC012946.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2151)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2151)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (20-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 28 Row: b Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: GenomeScan gene prediction
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..2151
/db_xref="H-InvDB:HIT000036052"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4454960"
/tissue_type="Kidney, hypernephroma"
/clone_lib="NIH_MGC_89"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2151
/gene="RGNEF"
/gene_synonym="FLJ21817"
/gene_synonym="KIAA1998"
/db_xref="GeneID:64283"
CDS 159..2006
/gene="RGNEF"
/gene_synonym="FLJ21817"
/gene_synonym="KIAA1998"
/codon_start=1
/product="RGNEF protein"
/protein_id="AAH12946.1"
/db_xref="GeneID:64283"
/translation="MIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQAL
MSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISL
QKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGG
RTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHL
EPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADT
LSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQS
EIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSR
DADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELL
LRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLL
PGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQFHSDL
VRTSEHQVDLLVDPSQPLNVSHKLWTAAGSGHQILPFHESSKDSCKNGN"
BASE COUNT 634 a 484 c 563 g 470 t
ORIGIN
1 atcttttggc tcgacgccga ggaattccag aatgcattct gttggtcact cagcgtatta
61 caaaataccc tgtcttggtg gaaaggatat tgcagtacac aaaggaaaga actgaggaac
121 ataaagactt acgcaaagcg ctttgcttaa ttaaagacat gattgcaaca gtggatttaa
181 aagtcaatga atatgagaaa aaccaaaaat ggcttgagat cctaaataag attgaaaaca
241 aaacatacac gaagctcaaa aatggacatg tgtttaggaa gcaggcactg atgagtgaag
301 aaaggactct gttatatgat ggccttgttt actggaaaac tgctacaggt cgtttcaaag
361 atatcctagc tctacttcta actgatgtgc tgctcttttt acaagaaaaa gaccagaaat
421 acatctttgc agccgttgat cagaagccat cagttatttc ccttcaaaag cttattgcta
481 gagaagttgc taatgaggag agaggaatgt ttctgatcag tgcttcatct gctggtcctg
541 agatgtatga aattcacacc aattccaagg aggaacgcaa taactggatg agacggatcc
601 agcaggctgt agaaagttgt cctgaagaaa aagggggaag gacaagtgaa tctgatgaag
661 acaagaggaa agctgaagcc agagtggcca aaattcagca atgtcaagaa atactcacta
721 accaagacca acaaatttgt gcgtatttgg aggagaagct gcatatctat gctgaacttg
781 gagaactgag cggatttgag gacgtccatc tagagcccca cctccttatt aaacctgacc
841 caggcgagcc tccccaggca gcctcattac tggcagcagc actgaaagaa gctgagagcc
901 tacaagttgc agtgaaggcc tcacagatgg gcgccgtgag tcaatcatgt gaggacagtt
961 gtggagactc tgtcttggcg gacacactca gttctcatga tgtaccagga tcaccgactg
1021 cctcattagt cacaggaggg agagaaggaa gaggctgttc ggatgtggat cccgggatcc
1081 agggtgtggt aaccgacttg gccgtctctg atgcagggga gaaggtggaa tgtagaaatt
1141 ttccaggttc ttcacaatca gagattatac aagccataca gaatttaacc cgtctcttat
1201 acagccttca ggccgccttg accattcagg acagccacat tgagatccac aggctggttc
1261 tccagcagca ggagggcctg tctctcggcc actctatcct ccgaggcggc cccttgcagg
1321 accagaagtc tcgcgacgcg gacaggcagc atgaggagct ggccaatgtg caccagcttc
1381 agcaccagct ccagcaggag cagcggcgct ggctgcgcag gtgtgagcag cagcagcggg
1441 cgcaggcgac cagggagagc tggctgcagg agcgggagcg ggagtgccag tcgcaggagg
1501 agctgctgct gcggagccgg ggcgagctgg acctccagct ccaggagtac cagcacagcc
1561 tggagcggct gagggagggc cagcgcctgg tggagaggga gcaggcgagg atgcgggccc
1621 agcagagcct gctgggccac tggaagcacg gccggcagag gagcctgccc gcggtgctcc
1681 ttccgggtgg ccccgaggta atggaactta accgatctga gagtttatgt catgaaaact
1741 cattcttcat caatgaagct ttagtacaaa tgtcatttaa cactttcaac aaactgaatc
1801 catcagttat ccatcaggat gccacttacc ctacaactca atttcattct gacttggtga
1861 ggactagtga acatcaagta gacctcctgg tggacccttc tcagcctttg aatgtcagtc
1921 acaaactgtg gacagccgct ggttccggcc atcagatact tcctttccat gaaagcagca
1981 aggattcttg taaaaatggt aattaacact ttaaacatca tctgtatagt ttgaacaaga
2041 agttgtccct ctgaatggtt ggctcttgtg tagagtgaat caaagtcctc cctagcatgg
2101 gaataaatcg gtactgatga aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
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